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The map label for this gene is smpB [H]

Identifier: 59712609

GI number: 59712609

Start: 2236874

End: 2237359

Strand: Direct

Name: smpB [H]

Synonym: VF_2002

Alternate gene names: 59712609

Gene position: 2236874-2237359 (Clockwise)

Preceding gene: 59712606

Following gene: 59712621

Centisome position: 77.2

GC content: 40.12

Gene sequence:

>486_bases
ATGGCAAAGAAAAAATCAAAAGATAAAGCGGGCAGTAATACCATTGCTATGAACAAGCAAGCTCGCCACGAATATTTCAT
TGATGATGAAATCGAAGCTGGGATTGAACTCCAAGGCTGGGAAGTTAAATCTCTTCGTTCTGGTAAAGTGAACATCGCTG
AAAGTTACGTTTACGTACGCGATGGCGAAATTTTTATTAGTGGTATGACAATCACACCGCTACAAGCAGCATCGACTCAC
GTTATTGCGAATCCAACTCGTATTCGTAAATTACTGATGAGCCGTAAAGAAATTGATAACCTTATTGGCCGTGTAAACCG
TGAAGGTATGACACTGGTTGCAACGACAATGTACTGGGTTCGTTCTTGGGCGAAAATCAAAGTTGGCGTAGCTAAAGGTA
AAAAACTGCACGATAAACGTACAGATTCAAAAGAAAAAGACTGGAACAGAGATAAAGCACGAATTATGAAGAGTAGTTTG
CGTTAA

Upstream 100 bases:

>100_bases
ACAACGTTAGGCGCTAAAAGTATGGTACTGAAAGGCTTAAGTTGTCTCGAGTTCAGGTTATCTAGTCAAACCACTTCCCT
ATCAGTATAATGTGGGCATT

Downstream 100 bases:

>100_bases
TTTCAACCAGTTAGATTGATACTAACTAGACAGACGCTGTTTTTCTGTTAGTATCATCTGGTAACAAAACTTTGGGGCTG
ATTCAGGATTCGACGGGAAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MAKKKSKDKAGSNTIAMNKQARHEYFIDDEIEAGIELQGWEVKSLRSGKVNIAESYVYVRDGEIFISGMTITPLQAASTH
VIANPTRIRKLLMSRKEIDNLIGRVNREGMTLVATTMYWVRSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSL
R

Sequences:

>Translated_161_residues
MAKKKSKDKAGSNTIAMNKQARHEYFIDDEIEAGIELQGWEVKSLRSGKVNIAESYVYVRDGEIFISGMTITPLQAASTH
VIANPTRIRKLLMSRKEIDNLIGRVNREGMTLVATTMYWVRSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSL
R
>Mature_160_residues
AKKKSKDKAGSNTIAMNKQARHEYFIDDEIEAGIELQGWEVKSLRSGKVNIAESYVYVRDGEIFISGMTITPLQAASTHV
IANPTRIRKLLMSRKEIDNLIGRVNREGMTLVATTMYWVRSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSLR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=63.3540372670807, Blast_Score=223, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18382; Mature: 18250

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKSKDKAGSNTIAMNKQARHEYFIDDEIEAGIELQGWEVKSLRSGKVNIAESYVYVR
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCCEECCCCCCCEEEEEEEEEEE
DGEIFISGMTITPLQAASTHVIANPTRIRKLLMSRKEIDNLIGRVNREGMTLVATTMYWV
CCEEEEECCEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
RSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSLR
HHHHHEEEECCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKKKSKDKAGSNTIAMNKQARHEYFIDDEIEAGIELQGWEVKSLRSGKVNIAESYVYVR
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCCEECCCCCCCEEEEEEEEEEE
DGEIFISGMTITPLQAASTHVIANPTRIRKLLMSRKEIDNLIGRVNREGMTLVATTMYWV
CCEEEEECCEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
RSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSLR
HHHHHEEEECCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA