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The map label for this gene is mltD [H]

Identifier: 59712546

GI number: 59712546

Start: 2167852

End: 2169405

Strand: Direct

Name: mltD [H]

Synonym: VF_1939

Alternate gene names: 59712546

Gene position: 2167852-2169405 (Clockwise)

Preceding gene: 172087738

Following gene: 59712547

Centisome position: 74.82

GC content: 41.25

Gene sequence:

>1554_bases
ATGAAACTGAAACTCCATTGGATTGGACTCGCATTATTAGCAGGCTGTCAAACGGCACCAACACCTGAAAATACACAGCC
TGATACTAACTCAGCAATCACCACGCCTTCAAAAACAGAGCCGAAAGCAGCAGAAGTTAAACCTGTTGAAAAACCGGCTC
CTACCCCTCAAGAACAAGAAGATGTTTGGCAACGCATTGCTATGCAACTTGAGATGCCAATTCCAGATAATGAGTCGGTT
GATTACTACCGTAATTGGTATATCAAACACCCAAGCCACCTTCGTACCGTTGCAAAGCGTGCAAAGCCATTCTTATATAT
GATCACGGTTGAAATTGAAAAGCGTGGTTTGCCTATGGAATTAGTTTTACTCCCTGTAGTAGAAAGTGCATTTGACCCAT
TCGCCTACTCTCATGGTAGTGCTGCAGGTTTATGGCAATTTGTTCCAGGTACCGCAGATCGATTTAAACTAGAGCGAAAC
TGGTGGTATGACGGACGCCGTGATGTACCAGCAGCAACAACCGCAGCCTTAGATTACATGGAATATTTCGACAGTCGCTT
TGATGGTAATTGGCAACATTCAATTGCCTCATACAACAGTGGCGCTGGCCGTGTATCTCGTTCAATTAAAAAGAACCAAA
GATTAGGTAAGCCAACTGACTTTTTCTCTTTAGATCTTCCAAAAGAGACCAGTGGTTATGTTCCAAAACTGCTTGCTTTA
GCTGATGTGATTAAAAACCACGAAAAATACGGCGTAGAGCTACCACCAATTCCTAACAAACCCGTTTTAGAAATGGTTGA
TCCTAAGCAGCAGTTAGATCTTGCCATTGCCGCAGAGTTTGCGGGTATTTCCATAACAGAATTACAAGGTTACAACCCAG
CTTATAATCAATGGGCAACCTCTCCCGATGGTCCATACCACTTATTACTGCCAATCGAACATGTTGACCAATTTAATGCC
AATTTGAAAAAGAACGCGAACAAAACAATGAAAGTGGTTCGCTATAAAGTTCAATCAGGCGATACCATCAGTCAATTAGC
AGTTAAGCACAATACGACAACCAAGATCATTGAACAAACCAATGACTTAAATGGTGCAAACATTCGTGTTGGTCAATATC
TATTGATCCCAACCGCTTCTAAAGGCAATAAAACATATGCGTTAAGTGCCCCGCAACGCTTGAAAAAAACGCAAGCAAAA
GCGAAAGGCAAATATAAGTTAACTCATAAAGTAAAAGACGGGGAAAGTCTGTGGAGTATTGCTCGTGATAACAGTATTTC
TTACCGAGATCTTGCTAAATGGAACGGCATGGCTCCAAGAGATTCTTTACGTGTAGGCCAAAACTTGGTGATTTGGAAAA
ATAGCTCAGATGGTGCAATCATTCGAACTGTTCATTATCAAATCAGACAAGGTGATAACCTAAGCTCTATCGCACAGAAA
TTCAGTGTCGCTGTGACAGATATTATGAAATGGAATAACATCGAACGAGGCTCTTACATTAAGCCGGGACAAAAATTAAA
ACTTTACGTTGATGTTACTAAGGTAAACGTATGA

Upstream 100 bases:

>100_bases
GAGTTTTAAGTATGAAACTTGTTTGATGTGTGGTTGCAAAGTATTATTCGCCTCCAAATAAAAATATTAATACGATTAAA
CACACTGAAGGCAATACATA

Downstream 100 bases:

>100_bases
CACCTTCTTCAAATCCATTGATCTTATTGGTCGATATATTTCGTTCACCAGCTGACTGTTTTGCTGCGCTATACGAGCGT
GGCAAATGGGCCTGGCTGCC

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKLKLHWIGLALLAGCQTAPTPENTQPDTNSAITTPSKTEPKAAEVKPVEKPAPTPQEQEDVWQRIAMQLEMPIPDNESV
DYYRNWYIKHPSHLRTVAKRAKPFLYMITVEIEKRGLPMELVLLPVVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERN
WWYDGRRDVPAATTAALDYMEYFDSRFDGNWQHSIASYNSGAGRVSRSIKKNQRLGKPTDFFSLDLPKETSGYVPKLLAL
ADVIKNHEKYGVELPPIPNKPVLEMVDPKQQLDLAIAAEFAGISITELQGYNPAYNQWATSPDGPYHLLLPIEHVDQFNA
NLKKNANKTMKVVRYKVQSGDTISQLAVKHNTTTKIIEQTNDLNGANIRVGQYLLIPTASKGNKTYALSAPQRLKKTQAK
AKGKYKLTHKVKDGESLWSIARDNSISYRDLAKWNGMAPRDSLRVGQNLVIWKNSSDGAIIRTVHYQIRQGDNLSSIAQK
FSVAVTDIMKWNNIERGSYIKPGQKLKLYVDVTKVNV

Sequences:

>Translated_517_residues
MKLKLHWIGLALLAGCQTAPTPENTQPDTNSAITTPSKTEPKAAEVKPVEKPAPTPQEQEDVWQRIAMQLEMPIPDNESV
DYYRNWYIKHPSHLRTVAKRAKPFLYMITVEIEKRGLPMELVLLPVVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERN
WWYDGRRDVPAATTAALDYMEYFDSRFDGNWQHSIASYNSGAGRVSRSIKKNQRLGKPTDFFSLDLPKETSGYVPKLLAL
ADVIKNHEKYGVELPPIPNKPVLEMVDPKQQLDLAIAAEFAGISITELQGYNPAYNQWATSPDGPYHLLLPIEHVDQFNA
NLKKNANKTMKVVRYKVQSGDTISQLAVKHNTTTKIIEQTNDLNGANIRVGQYLLIPTASKGNKTYALSAPQRLKKTQAK
AKGKYKLTHKVKDGESLWSIARDNSISYRDLAKWNGMAPRDSLRVGQNLVIWKNSSDGAIIRTVHYQIRQGDNLSSIAQK
FSVAVTDIMKWNNIERGSYIKPGQKLKLYVDVTKVNV
>Mature_517_residues
MKLKLHWIGLALLAGCQTAPTPENTQPDTNSAITTPSKTEPKAAEVKPVEKPAPTPQEQEDVWQRIAMQLEMPIPDNESV
DYYRNWYIKHPSHLRTVAKRAKPFLYMITVEIEKRGLPMELVLLPVVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERN
WWYDGRRDVPAATTAALDYMEYFDSRFDGNWQHSIASYNSGAGRVSRSIKKNQRLGKPTDFFSLDLPKETSGYVPKLLAL
ADVIKNHEKYGVELPPIPNKPVLEMVDPKQQLDLAIAAEFAGISITELQGYNPAYNQWATSPDGPYHLLLPIEHVDQFNA
NLKKNANKTMKVVRYKVQSGDTISQLAVKHNTTTKIIEQTNDLNGANIRVGQYLLIPTASKGNKTYALSAPQRLKKTQAK
AKGKYKLTHKVKDGESLWSIARDNSISYRDLAKWNGMAPRDSLRVGQNLVIWKNSSDGAIIRTVHYQIRQGDNLSSIAQK
FSVAVTDIMKWNNIERGSYIKPGQKLKLYVDVTKVNV

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=447, Percent_Identity=36.9127516778523, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58218; Mature: 58218

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLHWIGLALLAGCQTAPTPENTQPDTNSAITTPSKTEPKAAEVKPVEKPAPTPQEQE
CEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHH
DVWQRIAMQLEMPIPDNESVDYYRNWYIKHPSHLRTVAKRAKPFLYMITVEIEKRGLPME
HHHHHHHHEEECCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCEEEEEEEEEECCCCCEE
LVLLPVVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERNWWYDGRRDVPAATTAALDYM
EHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEECCCCCCCCCCCCHHHHHHHHHH
EYFDSRFDGNWQHSIASYNSGAGRVSRSIKKNQRLGKPTDFFSLDLPKETSGYVPKLLAL
HHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
ADVIKNHEKYGVELPPIPNKPVLEMVDPKQQLDLAIAAEFAGISITELQGYNPAYNQWAT
HHHHHCHHHCCCCCCCCCCCCHHHHCCCHHHHCEEEEEHHCCCEEEEECCCCCHHHCCCC
SPDGPYHLLLPIEHVDQFNANLKKNANKTMKVVRYKVQSGDTISQLAVKHNTTTKIIEQT
CCCCCEEEEEEHHHHHHHCCCHHHCCHHHEEEEEEECCCCCHHHHHHHHCCCHHHHHHHC
NDLNGANIRVGQYLLIPTASKGNKTYALSAPQRLKKTQAKAKGKYKLTHKVKDGESLWSI
CCCCCCEEEECEEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
ARDNSISYRDLAKWNGMAPRDSLRVGQNLVIWKNSSDGAIIRTVHYQIRQGDNLSSIAQK
HCCCCCCHHHHHHHCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEEEECCCCCHHHHHHH
FSVAVTDIMKWNNIERGSYIKPGQKLKLYVDVTKVNV
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MKLKLHWIGLALLAGCQTAPTPENTQPDTNSAITTPSKTEPKAAEVKPVEKPAPTPQEQE
CEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHH
DVWQRIAMQLEMPIPDNESVDYYRNWYIKHPSHLRTVAKRAKPFLYMITVEIEKRGLPME
HHHHHHHHEEECCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCEEEEEEEEEECCCCCEE
LVLLPVVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERNWWYDGRRDVPAATTAALDYM
EHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEECCCCCCCCCCCCHHHHHHHHHH
EYFDSRFDGNWQHSIASYNSGAGRVSRSIKKNQRLGKPTDFFSLDLPKETSGYVPKLLAL
HHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
ADVIKNHEKYGVELPPIPNKPVLEMVDPKQQLDLAIAAEFAGISITELQGYNPAYNQWAT
HHHHHCHHHCCCCCCCCCCCCHHHHCCCHHHHCEEEEEHHCCCEEEEECCCCCHHHCCCC
SPDGPYHLLLPIEHVDQFNANLKKNANKTMKVVRYKVQSGDTISQLAVKHNTTTKIIEQT
CCCCCEEEEEEHHHHHHHCCCHHHCCHHHEEEEEEECCCCCHHHHHHHHCCCHHHHHHHC
NDLNGANIRVGQYLLIPTASKGNKTYALSAPQRLKKTQAKAKGKYKLTHKVKDGESLWSI
CCCCCCEEEECEEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
ARDNSISYRDLAKWNGMAPRDSLRVGQNLVIWKNSSDGAIIRTVHYQIRQGDNLSSIAQK
HCCCCCCHHHHHHHCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEEEECCCCCHHHHHHH
FSVAVTDIMKWNNIERGSYIKPGQKLKLYVDVTKVNV
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]