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The map label for this gene is gltA [H]

Identifier: 59711425

GI number: 59711425

Start: 897006

End: 898295

Strand: Reverse

Name: gltA [H]

Synonym: VF_0818

Alternate gene names: 59711425

Gene position: 898295-897006 (Counterclockwise)

Preceding gene: 59711435

Following gene: 59711421

Centisome position: 31.0

GC content: 41.47

Gene sequence:

>1290_bases
ATGGCAGATAAAAAGGCAACACTTCATATTGAAGGTCAATCGCCTATAGACATGCCAATCATGGATGGAACGATTGGTCC
AGAAGTGATCGATGTGCGTAAATTAGGAGCGAATGGTTACTTTACTTTCGACCCTGGTTTTCTTGCCACTGCATCTTGTG
AGTCTCAAATTACCTATATCGATGGTGATAAAGGTGTTCTATTGCACCGTGGTTTCCCTATTGATCAGTTAGCTAACAAT
GCAGATTACCTTGAAGTCTGTTATATCCTTCTAAATGGCGAAGCGCCAAACAGAGAAGAATATGAAACGTTCAAGGACAT
CGTAACTCGCCACACAATGGTACATGAGCAAATTTCAAGCTTCTTCCATGGCTTCCGCCGTGATGCTCACCCTATGGCCG
TAATGTGTGGTGTTGTAGGAGCACTGGCTGCTTTCTATCACGATTCTTTAGATATCAATAACGATGAACACCGCGAAATT
GCTGCATTTAGATTACTATCAAAAATGCCAACTCTTTCAGCAATGTCTTACAAATACTCAATTGGACAACCGTTCATCTA
TCCTCGTAATGATCTGACTTACGCAGAAAACTTCTTACACATGATGTTCGCTAATCCGTGTGAAGAATATGAAGTAAACC
CTGTTGTTGCACGAGCAATGGATAAAATCTTTACGCTACATGCCGATCACGAACAAAATGCATCAACATCAACCGTTCGT
TTAGCTGGTTCATCAGGTGCGAATCCATTTGCTTGTATCGCTGCTGGTATTGCTTCTCTATGGGGACCTGCTCATGGTGG
TGCTAACGAAGCATGTTTACGTATGCTTGAAGAAATCGGCTCTGTAGACAACATTGATGAATACGTTGCAAAAGCAAAAG
ATAAGGATGACCCATTCCGCCTAATGGGCTTCGGTCACCGTGTTTATAAGAACTATGACCCTCGTGCAACCGTAATGCGT
GAAGCATGTCACGATGTTCTAAAAGAACTAAACATTAAAGATCCACTACTTGATGTCGCAATGGAACTTGAACGTATTGC
ACTTTCTGATGAATATTTTGTATCTAAGAAATTGTATCCAAACGTAGATTTCTACTCAGGTATTATCTTAAAAGCAATTG
GTATTCCTGTATCTATGTTTACGGTTATCTTTGCAATGTCTCGTACTGTTGGCTGGATTGCACACTGGAGCGAAATGCAC
AGTGATCCAACAAATCGTATCGGTCGTCCTCGCCAACTTTATACAGGTGAAGAACTACGCGAGTTTAAACCGCTTCATGA
GCGTGAATAA

Upstream 100 bases:

>100_bases
CACATAAGCGCACAAAAATTGACTTTAGCCCGTGTAATGGGTACAAAGTTGTCACTCTCATATCCGGGATAGAATAAAAA
TATAACAAAAGGAGAATGTT

Downstream 100 bases:

>100_bases
TGTCGGTTTAAATCGTTAGGCCTATGAACAAAAAGGTTGATGTAAATACATCAACCTTTTTTTATATCACCGTATTGATA
ACTCAAAATAGACTATACTG

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MADKKATLHIEGQSPIDMPIMDGTIGPEVIDVRKLGANGYFTFDPGFLATASCESQITYIDGDKGVLLHRGFPIDQLANN
ADYLEVCYILLNGEAPNREEYETFKDIVTRHTMVHEQISSFFHGFRRDAHPMAVMCGVVGALAAFYHDSLDINNDEHREI
AAFRLLSKMPTLSAMSYKYSIGQPFIYPRNDLTYAENFLHMMFANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGSVDNIDEYVAKAKDKDDPFRLMGFGHRVYKNYDPRATVMR
EACHDVLKELNIKDPLLDVAMELERIALSDEYFVSKKLYPNVDFYSGIILKAIGIPVSMFTVIFAMSRTVGWIAHWSEMH
SDPTNRIGRPRQLYTGEELREFKPLHERE

Sequences:

>Translated_429_residues
MADKKATLHIEGQSPIDMPIMDGTIGPEVIDVRKLGANGYFTFDPGFLATASCESQITYIDGDKGVLLHRGFPIDQLANN
ADYLEVCYILLNGEAPNREEYETFKDIVTRHTMVHEQISSFFHGFRRDAHPMAVMCGVVGALAAFYHDSLDINNDEHREI
AAFRLLSKMPTLSAMSYKYSIGQPFIYPRNDLTYAENFLHMMFANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGSVDNIDEYVAKAKDKDDPFRLMGFGHRVYKNYDPRATVMR
EACHDVLKELNIKDPLLDVAMELERIALSDEYFVSKKLYPNVDFYSGIILKAIGIPVSMFTVIFAMSRTVGWIAHWSEMH
SDPTNRIGRPRQLYTGEELREFKPLHERE
>Mature_428_residues
ADKKATLHIEGQSPIDMPIMDGTIGPEVIDVRKLGANGYFTFDPGFLATASCESQITYIDGDKGVLLHRGFPIDQLANNA
DYLEVCYILLNGEAPNREEYETFKDIVTRHTMVHEQISSFFHGFRRDAHPMAVMCGVVGALAAFYHDSLDINNDEHREIA
AFRLLSKMPTLSAMSYKYSIGQPFIYPRNDLTYAENFLHMMFANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVRL
AGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGSVDNIDEYVAKAKDKDDPFRLMGFGHRVYKNYDPRATVMRE
ACHDVLKELNIKDPLLDVAMELERIALSDEYFVSKKLYPNVDFYSGIILKAIGIPVSMFTVIFAMSRTVGWIAHWSEMHS
DPTNRIGRPRQLYTGEELREFKPLHERE

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=400, Percent_Identity=26.5, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786939, Length=423, Percent_Identity=75.886524822695, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=377, Percent_Identity=31.0344827586207, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=345, Percent_Identity=26.9565217391304, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319850, Length=363, Percent_Identity=27.2727272727273, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=326, Percent_Identity=26.9938650306748, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=414, Percent_Identity=26.0869565217391, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640126, Length=352, Percent_Identity=26.7045454545455, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640124, Length=352, Percent_Identity=26.7045454545455, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356863, Length=390, Percent_Identity=25.1282051282051, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48266; Mature: 48135

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKKATLHIEGQSPIDMPIMDGTIGPEVIDVRKLGANGYFTFDPGFLATASCESQITYI
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHEEECCCCEEEECCCCEEECCCCCEEEEE
DGDKGVLLHRGFPIDQLANNADYLEVCYILLNGEAPNREEYETFKDIVTRHTMVHEQISS
ECCCCEEEECCCCHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FFHGFRRDAHPMAVMCGVVGALAAFYHDSLDINNDEHREIAAFRLLSKMPTLSAMSYKYS
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHEEEEE
IGQPFIYPRNDLTYAENFLHMMFANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
CCCCEEECCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGSVDNIDEYVAKAKDKDDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCH
LMGFGHRVYKNYDPRATVMREACHDVLKELNIKDPLLDVAMELERIALSDEYFVSKKLYP
HHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCC
NVDFYSGIILKAIGIPVSMFTVIFAMSRTVGWIAHWSEMHSDPTNRIGRPRQLYTGEELR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHH
EFKPLHERE
HCCCCCCCC
>Mature Secondary Structure 
ADKKATLHIEGQSPIDMPIMDGTIGPEVIDVRKLGANGYFTFDPGFLATASCESQITYI
CCCCEEEEECCCCCCCCCCCCCCCCCCHHHHEEECCCCEEEECCCCEEECCCCCEEEEE
DGDKGVLLHRGFPIDQLANNADYLEVCYILLNGEAPNREEYETFKDIVTRHTMVHEQISS
ECCCCEEEECCCCHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FFHGFRRDAHPMAVMCGVVGALAAFYHDSLDINNDEHREIAAFRLLSKMPTLSAMSYKYS
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHEEEEE
IGQPFIYPRNDLTYAENFLHMMFANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
CCCCEEECCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEIGSVDNIDEYVAKAKDKDDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCH
LMGFGHRVYKNYDPRATVMREACHDVLKELNIKDPLLDVAMELERIALSDEYFVSKKLYP
HHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCC
NVDFYSGIILKAIGIPVSMFTVIFAMSRTVGWIAHWSEMHSDPTNRIGRPRQLYTGEELR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHH
EFKPLHERE
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]