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The map label for this gene is ybfF [C]

Identifier: 59711421

GI number: 59711421

Start: 892984

End: 893760

Strand: Reverse

Name: ybfF [C]

Synonym: VF_0814

Alternate gene names: 59711421

Gene position: 893760-892984 (Counterclockwise)

Preceding gene: 59711425

Following gene: 59711420

Centisome position: 30.85

GC content: 36.81

Gene sequence:

>777_bases
ATGATGTTACTTAACTATAAAGAACAAGGAAATGGGGATCCTTTGATCCTAATTCATGGATTATTTGGAAGCTTAGACAA
CTTAGGCCTTCTTGCTCGACAATTTGAAACAGAATATCGAGTGATCAGTGTTGATCTTCGAAATCACGGTCGTTCATTCC
ACTCGGATATTCATAATTATCATGAACTTGCAAACGATCTTGTTAATTTATTCGATCATTTATCTATCACTTCAGCGCAT
ATTGTTGGCCATTCAATGGGTGGGAAAGTAGCAATGGCGCTAGCGAGTCAATCACCACAATTGATTCAATCTCTATCAAT
TTTAGACATGGCTCCTGTTGCTTACCCTGAGAGAAAACATGATGCTGTATTCTCAGGTTTGCACAAGATCCTTAATAATA
AACCAACAACACGTAAAGAAGCAGACGACTTACTTGCCACCTCTATTGTTGATCCTGGTGTTAGACAATTTTTAAGTAAA
TCACTATATAAAGAACAAGAGCACCTTACTCTTCGCTTTAATGTGAATGCTTTATTTGATAATTACGCACAGATTATTGG
CTGGGATAATATTCCCGCTTTTGAAGGTGATGTTATCTTTATTAAGGGGCAAAACTCAGACTATATTTTAGCTGAACATC
AAGCGAATATTATAAGCCAATTCCCAAATGCCAAAGCGCACATTGTTAACGGAACTGGACATTGGTTACATGCAGAAAAG
CCCGAAACAATTTATCGAGTCATAAAACGCTTCATTGAAAAAGTGACAAATCGATAA

Upstream 100 bases:

>100_bases
TTCATTATATGGTTCGTATTCTATAATCTATTTATGAAGGCACGATTATACCCTGTAAAATCGTGATACTCTACGACAAA
CCTAACACAACGATAGAAAA

Downstream 100 bases:

>100_bases
TCCATACCAACAAAGACAAAGCTATGATATAGTTTTGCAAAATTATTCGATCAGATATAGGTCGCTTACTTAAGATAAGG
GTGATTCATGCTTTATGAAC

Product: putative esterase/lipase YbfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MMLLNYKEQGNGDPLILIHGLFGSLDNLGLLARQFETEYRVISVDLRNHGRSFHSDIHNYHELANDLVNLFDHLSITSAH
IVGHSMGGKVAMALASQSPQLIQSLSILDMAPVAYPERKHDAVFSGLHKILNNKPTTRKEADDLLATSIVDPGVRQFLSK
SLYKEQEHLTLRFNVNALFDNYAQIIGWDNIPAFEGDVIFIKGQNSDYILAEHQANIISQFPNAKAHIVNGTGHWLHAEK
PETIYRVIKRFIEKVTNR

Sequences:

>Translated_258_residues
MMLLNYKEQGNGDPLILIHGLFGSLDNLGLLARQFETEYRVISVDLRNHGRSFHSDIHNYHELANDLVNLFDHLSITSAH
IVGHSMGGKVAMALASQSPQLIQSLSILDMAPVAYPERKHDAVFSGLHKILNNKPTTRKEADDLLATSIVDPGVRQFLSK
SLYKEQEHLTLRFNVNALFDNYAQIIGWDNIPAFEGDVIFIKGQNSDYILAEHQANIISQFPNAKAHIVNGTGHWLHAEK
PETIYRVIKRFIEKVTNR
>Mature_258_residues
MMLLNYKEQGNGDPLILIHGLFGSLDNLGLLARQFETEYRVISVDLRNHGRSFHSDIHNYHELANDLVNLFDHLSITSAH
IVGHSMGGKVAMALASQSPQLIQSLSILDMAPVAYPERKHDAVFSGLHKILNNKPTTRKEADDLLATSIVDPGVRQFLSK
SLYKEQEHLTLRFNVNALFDNYAQIIGWDNIPAFEGDVIFIKGQNSDYILAEHQANIISQFPNAKAHIVNGTGHWLHAEK
PETIYRVIKRFIEKVTNR

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=246, Percent_Identity=33.739837398374, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI23200012, Length=246, Percent_Identity=33.739837398374, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI223941839, Length=241, Percent_Identity=29.045643153527, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786902, Length=246, Percent_Identity=41.869918699187, Blast_Score=222, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17508535, Length=260, Percent_Identity=32.3076923076923, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17507063, Length=270, Percent_Identity=28.1481481481481, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321468, Length=267, Percent_Identity=32.5842696629214, Blast_Score=109, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24640348, Length=259, Percent_Identity=28.957528957529, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646055, Length=268, Percent_Identity=24.2537313432836, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012908 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07819 PGAP1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 29115; Mature: 29115

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLLNYKEQGNGDPLILIHGLFGSLDNLGLLARQFETEYRVISVDLRNHGRSFHSDIHNY
CEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEHHHCCCHHHHHHHHH
HELANDLVNLFDHLSITSAHIVGHSMGGKVAMALASQSPQLIQSLSILDMAPVAYPERKH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCCCCHH
DAVFSGLHKILNNKPTTRKEADDLLATSIVDPGVRQFLSKSLYKEQEHLTLRFNVNALFD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHH
NYAQIIGWDNIPAFEGDVIFIKGQNSDYILAEHQANIISQFPNAKAHIVNGTGHWLHAEK
HHHHHHCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCCEEEECCCCCEEECCC
PETIYRVIKRFIEKVTNR
CHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMLLNYKEQGNGDPLILIHGLFGSLDNLGLLARQFETEYRVISVDLRNHGRSFHSDIHNY
CEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEHHHCCCHHHHHHHHH
HELANDLVNLFDHLSITSAHIVGHSMGGKVAMALASQSPQLIQSLSILDMAPVAYPERKH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCCCCHH
DAVFSGLHKILNNKPTTRKEADDLLATSIVDPGVRQFLSKSLYKEQEHLTLRFNVNALFD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHH
NYAQIIGWDNIPAFEGDVIFIKGQNSDYILAEHQANIISQFPNAKAHIVNGTGHWLHAEK
HHHHHHCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCCEEEECCCCCEEECCC
PETIYRVIKRFIEKVTNR
CHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]