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The map label for this gene is proW [H]

Identifier: 59711393

GI number: 59711393

Start: 859679

End: 860761

Strand: Reverse

Name: proW [H]

Synonym: VF_0786

Alternate gene names: 59711393

Gene position: 860761-859679 (Counterclockwise)

Preceding gene: 59711394

Following gene: 59711392

Centisome position: 29.71

GC content: 39.61

Gene sequence:

>1083_bases
ATGCCAACAAATCAAACAACAAATGATCCATGGTCACAAACTGAAACTCAATCAAACGATCCTTGGGGTCAAGCAAGCGA
ATCAGCATCTTCAAACGATTGGTTAACAACAGAAACAGTAGACACTAAATCTTTTGATATTTTAAACCCATTTGAAGAAG
CGGTTCTTCCTGTAGATACATGGGTTGAATCTGGATTAAATTGGTTAGTAGAGCATGGTCGCCCCCTATTTCAAGCAATA
CGTGTTCCTATCGACTTTATTTTAAGCTCTTTTGAAACCGCTCTTGTATCAACACCTGCGCCATTCATGTTGCTTATTTT
ATTTTTACTTGCGTGGCAATTTTCAAATCTAAAGCTTGGTGTATCCACTGCAATTTCACTGTTATTTATAGGACTTATCG
GTGCTTGGTCACAAGCAATGACGACCCTTGCTCTGGTTATGACCTCTGTGTTTTTCTGTTTACTTATCGGACTCCCTATG
GGGATCTGGTTAGCAAGAAGTCGCACTGCTGCCAAATTTATTAGACCTATCTTAGATGCAATGCAAACCACCCCTGCATT
CGTTTATTTAGTTCCAATAGTTATGCTATTTGGTATTGGTAATGTTCCAGGTGTAGTCGTAACCATCATTTTTGCTCTAC
CTCCGGTTGTTCGTTTAACCATACTCGGTATTCAGCAAGTACCTACCGACCTTATTGAAGCTGGACATTCTTTTGGGGCG
AATAAAAAACAGATGCTTTATCGGATCCAATTACCTCTCGCGCTGCCAACTATCATGGCTGGTGTAAACCAAACCTTAAT
GCTATCTCTGTCTATGGTTGTTATCGCATCTATGATTGCTGTAGGTGGTTTAGGTCAAATGGTATTACGAGGCATTGGAC
GATTGGATATGGGGTTAGCTGCCGTTGGTGGTCTAGGTATTGTTATCCTTGCCATTTTACTCGATAGAATCACTCAAATA
TTAGGTGTAAACGCAGGCAATACCAAATTACGTTGGTATCAAATAGGACCTGTCGCCATCTTACTAAAAATATTTGCTCA
AAAAAAAGAACCAAAACAAGAACTGAAACTTAAGGAGAAATAA

Upstream 100 bases:

>100_bases
TGACGTTCCTTATTCCGTTCCTGTCGTTGACAAAAACGGTAATTACTTTGGTGTTGTGACTAAATCACGACTTCTACAAA
CGTTAGATAAGGGATAAGTC

Downstream 100 bases:

>100_bases
GAATGATTAATTCATGGAAGAAAGCACTAACAGCCAGTATCGTTTCGACATTAGCTTTATCTACTAATGTTTGGGCTGAA
AAGCTTCCAGGAGAAGGAGT

Product: glycine betaine transporter subunit

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPTNQTTNDPWSQTETQSNDPWGQASESASSNDWLTTETVDTKSFDILNPFEEAVLPVDTWVESGLNWLVEHGRPLFQAI
RVPIDFILSSFETALVSTPAPFMLLILFLLAWQFSNLKLGVSTAISLLFIGLIGAWSQAMTTLALVMTSVFFCLLIGLPM
GIWLARSRTAAKFIRPILDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPTDLIEAGHSFGA
NKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRITQI
LGVNAGNTKLRWYQIGPVAILLKIFAQKKEPKQELKLKEK

Sequences:

>Translated_360_residues
MPTNQTTNDPWSQTETQSNDPWGQASESASSNDWLTTETVDTKSFDILNPFEEAVLPVDTWVESGLNWLVEHGRPLFQAI
RVPIDFILSSFETALVSTPAPFMLLILFLLAWQFSNLKLGVSTAISLLFIGLIGAWSQAMTTLALVMTSVFFCLLIGLPM
GIWLARSRTAAKFIRPILDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPTDLIEAGHSFGA
NKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRITQI
LGVNAGNTKLRWYQIGPVAILLKIFAQKKEPKQELKLKEK
>Mature_359_residues
PTNQTTNDPWSQTETQSNDPWGQASESASSNDWLTTETVDTKSFDILNPFEEAVLPVDTWVESGLNWLVEHGRPLFQAIR
VPIDFILSSFETALVSTPAPFMLLILFLLAWQFSNLKLGVSTAISLLFIGLIGAWSQAMTTLALVMTSVFFCLLIGLPMG
IWLARSRTAAKFIRPILDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPTDLIEAGHSFGAN
KKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRITQIL
GVNAGNTKLRWYQIGPVAILLKIFAQKKEPKQELKLKEK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=350, Percent_Identity=62.2857142857143, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=29.2307692307692, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=32.4675324675325, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788449, Length=187, Percent_Identity=27.807486631016, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 39254; Mature: 39123

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTNQTTNDPWSQTETQSNDPWGQASESASSNDWLTTETVDTKSFDILNPFEEAVLPVDT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCHHH
WVESGLNWLVEHGRPLFQAIRVPIDFILSSFETALVSTPAPFMLLILFLLAWQFSNLKLG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEC
VSTAISLLFIGLIGAWSQAMTTLALVMTSVFFCLLIGLPMGIWLARSRTAAKFIRPILDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPTDLIEAGHSFGA
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
NKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLA
CHHHEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
AVGGLGIVILAILLDRITQILGVNAGNTKLRWYQIGPVAILLKIFAQKKEPKQELKLKEK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHCCCCCC
>Mature Secondary Structure 
PTNQTTNDPWSQTETQSNDPWGQASESASSNDWLTTETVDTKSFDILNPFEEAVLPVDT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCHHH
WVESGLNWLVEHGRPLFQAIRVPIDFILSSFETALVSTPAPFMLLILFLLAWQFSNLKLG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEC
VSTAISLLFIGLIGAWSQAMTTLALVMTSVFFCLLIGLPMGIWLARSRTAAKFIRPILDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPTDLIEAGHSFGA
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
NKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLA
CHHHEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
AVGGLGIVILAILLDRITQILGVNAGNTKLRWYQIGPVAILLKIFAQKKEPKQELKLKEK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]