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The map label for this gene is ipk

Identifier: 59711372

GI number: 59711372

Start: 835421

End: 836287

Strand: Reverse

Name: ipk

Synonym: VF_0765

Alternate gene names: 59711372

Gene position: 836287-835421 (Counterclockwise)

Preceding gene: 59711373

Following gene: 59711371

Centisome position: 28.86

GC content: 40.37

Gene sequence:

>867_bases
ATGATCACCAAAACAACTCGCTGGCCATCCCCAGCCAAATTAAACCTATTTCTTTATATCAATGGCCAACAAGAAAATGG
CTACCATGAGCTTCAAACTTTATTTCAATTTATTGATTTATGTGATCACCTCACCATTACTGCTAATCAATCAGGTCAAA
TAACCCTTGCACCAGACATTCCTGGAGTAAAAACTGAAGATAATCTGATATGGAAAGCGGCAACGCTATTACAGCAGCAC
AGTCAATGTGAATTCGGTGCTCATATCGAAATAGAAAAGATTTTACCTATGGGTGGAGGCATCGGTGGTGGTTCCTCTAA
TGCAGCAACCGTACTCGTCGCCCTTAATTTTCTATGGCAGCTCAATTTAAGTAACGATACTTTAGCAGCACTCGGAGTAA
AACTAGGGGCTGATGTGCCTATTTTTGTTCATGGATTTGCCGCTTTTGCAGAAGGTATTGGTGAGAAATTGCAACCAGCC
ACCCCTAAAGAGTTATGGTACGTTTTAATCAAACCAGAAGTCAGTATCGCAACCGTCGATGTTTTCACTCATCCTAACTT
GGTCAGAAATACACCAAAACAGCCACTTAACGCACTTTTAGAGGCAACATACGTAAACGATTGCGAAAAAATTGTCAGAA
GTGTGTATCCAGAGGTTGATTACCAACTTTCATGGCTGTTAGAATATGCGCCATCGAGATTAACTGGAACAGGCGCTTGT
GTTTTTGCTGAATTTAACAGCGAAAAAGAAGCACAAGATGTTTATTCTTTAATCCCTGATAATGCAACCGGATTTATCGC
CCGAGGTATGAATACTTCGCCACTCAACCGAACACTGGAAGAGTATAAATCATTATGCAAAATATAA

Upstream 100 bases:

>100_bases
GCTATATTGAAAAAAGTAACCTACTTCTCCCTAATTCAATGAAACTCACTCAACAAGATACCACCATTAAAATCATTGCT
TCAAATTGGATAATTTCTCA

Downstream 100 bases:

>100_bases
AATTCTCACAATTTGGACGCAACCCTGAGGTTTCCACCGTGCCTGACATGAAGCTATTTGCTGGTAATGCAACTCCTGAA
CTAGCCCAACGTATCGCTGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MITKTTRWPSPAKLNLFLYINGQQENGYHELQTLFQFIDLCDHLTITANQSGQITLAPDIPGVKTEDNLIWKAATLLQQH
SQCEFGAHIEIEKILPMGGGIGGGSSNAATVLVALNFLWQLNLSNDTLAALGVKLGADVPIFVHGFAAFAEGIGEKLQPA
TPKELWYVLIKPEVSIATVDVFTHPNLVRNTPKQPLNALLEATYVNDCEKIVRSVYPEVDYQLSWLLEYAPSRLTGTGAC
VFAEFNSEKEAQDVYSLIPDNATGFIARGMNTSPLNRTLEEYKSLCKI

Sequences:

>Translated_288_residues
MITKTTRWPSPAKLNLFLYINGQQENGYHELQTLFQFIDLCDHLTITANQSGQITLAPDIPGVKTEDNLIWKAATLLQQH
SQCEFGAHIEIEKILPMGGGIGGGSSNAATVLVALNFLWQLNLSNDTLAALGVKLGADVPIFVHGFAAFAEGIGEKLQPA
TPKELWYVLIKPEVSIATVDVFTHPNLVRNTPKQPLNALLEATYVNDCEKIVRSVYPEVDYQLSWLLEYAPSRLTGTGAC
VFAEFNSEKEAQDVYSLIPDNATGFIARGMNTSPLNRTLEEYKSLCKI
>Mature_288_residues
MITKTTRWPSPAKLNLFLYINGQQENGYHELQTLFQFIDLCDHLTITANQSGQITLAPDIPGVKTEDNLIWKAATLLQQH
SQCEFGAHIEIEKILPMGGGIGGGSSNAATVLVALNFLWQLNLSNDTLAALGVKLGADVPIFVHGFAAFAEGIGEKLQPA
TPKELWYVLIKPEVSIATVDVFTHPNLVRNTPKQPLNALLEATYVNDCEKIVRSVYPEVDYQLSWLLEYAPSRLTGTGAC
VFAEFNSEKEAQDVYSLIPDNATGFIARGMNTSPLNRTLEEYKSLCKI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=280, Percent_Identity=57.8571428571429, Blast_Score=303, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_VIBF1 (Q5E6T6)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204148.1
- ProteinModelPortal:   Q5E6T6
- SMR:   Q5E6T6
- STRING:   Q5E6T6
- GeneID:   3277416
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0765
- NMPDR:   fig|312309.3.peg.1266
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   Q5E6T6
- ProtClustDB:   PRK00343
- BioCyc:   VFIS312309:VF0765-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31711; Mature: 31711

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKTTRWPSPAKLNLFLYINGQQENGYHELQTLFQFIDLCDHLTITANQSGQITLAPDI
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCC
PGVKTEDNLIWKAATLLQQHSQCEFGAHIEIEKILPMGGGIGGGSSNAATVLVALNFLWQ
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHEEEEHHEE
LNLSNDTLAALGVKLGADVPIFVHGFAAFAEGIGEKLQPATPKELWYVLIKPEVSIATVD
EECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEE
VFTHPNLVRNTPKQPLNALLEATYVNDCEKIVRSVYPEVDYQLSWLLEYAPSRLTGTGAC
EECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEE
VFAEFNSEKEAQDVYSLIPDNATGFIARGMNTSPLNRTLEEYKSLCKI
EEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITKTTRWPSPAKLNLFLYINGQQENGYHELQTLFQFIDLCDHLTITANQSGQITLAPDI
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCC
PGVKTEDNLIWKAATLLQQHSQCEFGAHIEIEKILPMGGGIGGGSSNAATVLVALNFLWQ
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHEEEEHHEE
LNLSNDTLAALGVKLGADVPIFVHGFAAFAEGIGEKLQPATPKELWYVLIKPEVSIATVD
EECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEE
VFTHPNLVRNTPKQPLNALLEATYVNDCEKIVRSVYPEVDYQLSWLLEYAPSRLTGTGAC
EECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEE
VFAEFNSEKEAQDVYSLIPDNATGFIARGMNTSPLNRTLEEYKSLCKI
EEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA