Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 59711366

GI number: 59711366

Start: 827525

End: 828949

Strand: Reverse

Name: miaB

Synonym: VF_0759

Alternate gene names: 59711366

Gene position: 828949-827525 (Counterclockwise)

Preceding gene: 172087678

Following gene: 59711365

Centisome position: 28.61

GC content: 41.82

Gene sequence:

>1425_bases
ATGACTAAGAAACTGCTGATCAAAACCTGGGGCTGTCAGATGAACGAATACGATTCATCTAAAATGGCCGACCTTTTAGG
TGCCGCAAATGGTTATGAATTAACAGAAGAACCAACAGAAGCTGACGTTCTACTATTAAATACCTGCTCTATTCGTGAAA
AAGCACAAGAGAAAGTGTTTCACCAATTAGGCCGCTGGAAAAACCTAAAAGATAAAAAACCAGATCTAGTGATCGGTGTT
GGTGGTTGTGTTGCAACTCAAGAAGGTGATCATATTCGCCAACGAGCTCCATACGTTGACGTAATTTTTGGTCCACAAAC
TTTACACCGTTTACCTGAAATGATCCGCCAATCTCAATCAAATGAAAAACCAGTAATGGATATTTCATTCCCAGAGATCG
AAAAGTTCGATAACCTTCCTGAACCAAAAGCAGAAGGTGCAACAGCGTTCGTTTCTATTATGGAAGGGTGTTCTAAGTAC
TGTACTTACTGTGTTGTTCCATACACTCGTGGTGAAGAAGTAAGCCGTCCTCTTGATGACGTATTATTTGAAATTGCACA
GCTTGCAGAGCAAGGCGTACGTGAAGTAAACCTATTGGGTCAAAACGTTAACGCGTACCGTGGTCCAATGCACGACGGTG
ATATCTGCACATTTGCTGAATTATTACGTATGGTTGCATCTATCGATGGTATCGACCGTTTACGCTTTACAACAAGCCAC
CCATTAGAGTTTGGTGATGACATCATCGCTGTATACGAAGATACGCCAGAGCTAGTAAGTTTCTTACACTTACCTGTTCA
AAGTGGTTCTGATCGTATTCTAACTATGATGAAGCGCCCTCACACAGCGATTGAATACAAATCAATCATTCGTAAATTAC
GTAAGGCTCGTCCTGATATTCAAATCAGTTCTGACTTTATTGTTGGCTTCCCTGGTGAAACGGCGAAAGACTTCCAAGAT
ACAATGAAACTGATTAAAGATGTCGACTTCGACATGAGCTTTAGTTTCATCTTCTCTGCGCGTCCAGGAACACCTGCTGC
TGATTATCCATGTGATATCCCTGAACAAGAGAAGAAAGATCGCCTAGCTGAACTGCAACAGCAAGTAAACAGCCAAGCAA
TGCGCTACTCTCGTCTAATGCTAGACACTGAGCAACGTGTATTAGTTGAAGGCCCATCGAAGAAAAACCTGATGGAACTT
CGTGCTCGTACTGAAAACAACCGTGTTGTAAACTTTGAAGGTTCAGCGGATCTTATTGGTCAATTTGTTGATGTTAAAAT
CACAGATGTATTTGCGAACTCACTGCGTGGTGAACTAGTTCGTACAGAAAAAGACATGGGTTTACGTGTTGTTATGACTC
CAGCAGAAATGATGGAAAAGACACGTCGTGAAGATGATCTTGGTGTAGGTACTTTTACGCCATAG

Upstream 100 bases:

>100_bases
CGCTTGGAAAGCAGTACAATACGCGCCCTATCCGTTTTGATCGAGCTTGTCTCTCAAAACTGTACCGAATTTTAGTAATA
CAAAACGAGTGAAAAGTTAC

Downstream 100 bases:

>100_bases
TGTGATCTCTTTAATAAAATCAAAGACAAATAAAGATGATTGAGGTTACTCAGTCATCTTTTTTCTTTTATAGTCTTGAG
AAAATACCTTATTTACCCAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEEPTEADVLLLNTCSIREKAQEKVFHQLGRWKNLKDKKPDLVIGV
GGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIRQSQSNEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPLDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGDICTFAELLRMVASIDGIDRLRFTTSH
PLEFGDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGETAKDFQD
TMKLIKDVDFDMSFSFIFSARPGTPAADYPCDIPEQEKKDRLAELQQQVNSQAMRYSRLMLDTEQRVLVEGPSKKNLMEL
RARTENNRVVNFEGSADLIGQFVDVKITDVFANSLRGELVRTEKDMGLRVVMTPAEMMEKTRREDDLGVGTFTP

Sequences:

>Translated_474_residues
MTKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEEPTEADVLLLNTCSIREKAQEKVFHQLGRWKNLKDKKPDLVIGV
GGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIRQSQSNEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPLDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGDICTFAELLRMVASIDGIDRLRFTTSH
PLEFGDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGETAKDFQD
TMKLIKDVDFDMSFSFIFSARPGTPAADYPCDIPEQEKKDRLAELQQQVNSQAMRYSRLMLDTEQRVLVEGPSKKNLMEL
RARTENNRVVNFEGSADLIGQFVDVKITDVFANSLRGELVRTEKDMGLRVVMTPAEMMEKTRREDDLGVGTFTP
>Mature_473_residues
TKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEEPTEADVLLLNTCSIREKAQEKVFHQLGRWKNLKDKKPDLVIGVG
GCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIRQSQSNEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKYC
TYCVVPYTRGEEVSRPLDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGDICTFAELLRMVASIDGIDRLRFTTSHP
LEFGDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGETAKDFQDT
MKLIKDVDFDMSFSFIFSARPGTPAADYPCDIPEQEKKDRLAELQQQVNSQAMRYSRLMLDTEQRVLVEGPSKKNLMELR
ARTENNRVVNFEGSADLIGQFVDVKITDVFANSLRGELVRTEKDMGLRVVMTPAEMMEKTRREDDLGVGTFTP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=35.3430353430353, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=35.1351351351351, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=29.5404814004376, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=76.5822784810127, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=383, Percent_Identity=28.9817232375979, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=32.3974082073434, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=28.9808917197452, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=34.5911949685535, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=30.5084745762712, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_VIBF1 (Q5E6U2)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204142.1
- ProteinModelPortal:   Q5E6U2
- SMR:   Q5E6U2
- STRING:   Q5E6U2
- GeneID:   3277402
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0759
- NMPDR:   fig|312309.3.peg.1260
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q5E6U2
- ProtClustDB:   PRK14325
- BioCyc:   VFIS312309:VF0759-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53611; Mature: 53480

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEEPTEADVLLLNTCSIREKAQEKVF
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKNLKDKKPDLVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIRQSQS
HHHHCCCCCCCCCCCEEEECCCEEECCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHCCC
NEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPLDD
CCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGDICTFAELLRMVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PLEFGDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETAKDFQDTMKLIKDVDFDMSFSFIFSARPGTPAADYPCDIPEQEKKD
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLAELQQQVNSQAMRYSRLMLDTEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHH
QFVDVKITDVFANSLRGELVRTEKDMGLRVVMTPAEMMEKTRREDDLGVGTFTP
HHHCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEEPTEADVLLLNTCSIREKAQEKVF
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKNLKDKKPDLVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIRQSQS
HHHHCCCCCCCCCCCEEEECCCEEECCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHCCC
NEKPVMDISFPEIEKFDNLPEPKAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPLDD
CCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGDICTFAELLRMVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PLEFGDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETAKDFQDTMKLIKDVDFDMSFSFIFSARPGTPAADYPCDIPEQEKKD
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLAELQQQVNSQAMRYSRLMLDTEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHH
QFVDVKITDVFANSLRGELVRTEKDMGLRVVMTPAEMMEKTRREDDLGVGTFTP
HHHCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA