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The map label for this gene is holA [H]

Identifier: 59711358

GI number: 59711358

Start: 818161

End: 819195

Strand: Reverse

Name: holA [H]

Synonym: VF_0751

Alternate gene names: 59711358

Gene position: 819195-818161 (Counterclockwise)

Preceding gene: 59711359

Following gene: 59711357

Centisome position: 28.27

GC content: 38.45

Gene sequence:

>1035_bases
ATGCGTGTATTCCCTGAACAATTAGCTCAAAACTTAGAACGTGGGCTACGTCAATGCTATTTGCTGTTTGGTAATGAGCC
TCTGTTAAAACAAGAAAGCCTTGATGCAATTCGCCAAGCGGCATTTACTCAAGGTTTTGAAGAAAAACACCAATTTACAC
TGGATAAGCAGTTAGATTGGAATAACGTATTTGATTGCACTCAAGCGTTAAGCCTTTTTTCATCTAGACAAATAATAGAG
CTAACCATTCCTGACACTGGAATGACAACCGCACATACAAGCAAGCTAAAAGAACTCGTACCAACACTGCATGACGATAT
AATTTTATTGTTACAAGGCCCAAGAGTTAATAAAGCTCAAGAAAACACCCAGTGGTTTAAGGCGTTAACGCAGTTAGGTT
TATTTATTCCATGTAATACACCTGACAATCAGCAGTTACCTCGTTTTATCCAACAACGTTGTAAAAAGTTAAAGCTAAAA
CCTGATGCAGAAGCGATTCAAATGCTTGCCCAATGGCACGAAGGCAATTTATTAGCGCTTGCTCAAAGCCTAGATAAATT
AGCCCTTTTATATCCTGATGGCTTACTAACATTAATTCGAATAGAAGAAGCCTTAAATAGAAACAATCATTTTACGCCGT
TTCAATTGGTCGACACGTTATTATCAGGTCAAGCCAAACGTGCACAGCGCATTCTTCGTCAACTTGAAAGTGAAGGAACA
GAAGTCATCATTCTTCTGCGCACATTACAAAAAGAGCTACTGTTACTACAAACATTCCAATATTCGCTATCTCAAGGCGA
AGCAATGTTTACTGTTTTTGATCGATATAAAGTATGGCAAAACCGCCGAGCACTATATCAAGCAGCTTTACAGCGCTTAT
CAATAACTCAAATAAAACAATTAATTCGCTTACTTGCACAGATCGAAATAGCGACAAAAACAGACTATGACAGTGATTGC
TGGCCACAACTTAGCCAACTAACGCTAGATATGTGCAATATTCACATTTCGGTGACATCGAACGTCTCTCATTAA

Upstream 100 bases:

>100_bases
CAAGCGATGTAATTACGCACGATGATGAAACTGAAAGTAAAGTGACAACGAAAGAAGTCATAAAATCAATGAGCACTACA
GCTGAAACAACTCAATAATC

Downstream 100 bases:

>100_bases
GCGGCGTTCACTGATATACTTCATTATAAATAACCCATTTCTAGGTGATGACTTTGCAAGATAAAGAACTCCATGATTTT
CTATTCAACAACGTTGATGA

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRVFPEQLAQNLERGLRQCYLLFGNEPLLKQESLDAIRQAAFTQGFEEKHQFTLDKQLDWNNVFDCTQALSLFSSRQIIE
LTIPDTGMTTAHTSKLKELVPTLHDDIILLLQGPRVNKAQENTQWFKALTQLGLFIPCNTPDNQQLPRFIQQRCKKLKLK
PDAEAIQMLAQWHEGNLLALAQSLDKLALLYPDGLLTLIRIEEALNRNNHFTPFQLVDTLLSGQAKRAQRILRQLESEGT
EVIILLRTLQKELLLLQTFQYSLSQGEAMFTVFDRYKVWQNRRALYQAALQRLSITQIKQLIRLLAQIEIATKTDYDSDC
WPQLSQLTLDMCNIHISVTSNVSH

Sequences:

>Translated_344_residues
MRVFPEQLAQNLERGLRQCYLLFGNEPLLKQESLDAIRQAAFTQGFEEKHQFTLDKQLDWNNVFDCTQALSLFSSRQIIE
LTIPDTGMTTAHTSKLKELVPTLHDDIILLLQGPRVNKAQENTQWFKALTQLGLFIPCNTPDNQQLPRFIQQRCKKLKLK
PDAEAIQMLAQWHEGNLLALAQSLDKLALLYPDGLLTLIRIEEALNRNNHFTPFQLVDTLLSGQAKRAQRILRQLESEGT
EVIILLRTLQKELLLLQTFQYSLSQGEAMFTVFDRYKVWQNRRALYQAALQRLSITQIKQLIRLLAQIEIATKTDYDSDC
WPQLSQLTLDMCNIHISVTSNVSH
>Mature_344_residues
MRVFPEQLAQNLERGLRQCYLLFGNEPLLKQESLDAIRQAAFTQGFEEKHQFTLDKQLDWNNVFDCTQALSLFSSRQIIE
LTIPDTGMTTAHTSKLKELVPTLHDDIILLLQGPRVNKAQENTQWFKALTQLGLFIPCNTPDNQQLPRFIQQRCKKLKLK
PDAEAIQMLAQWHEGNLLALAQSLDKLALLYPDGLLTLIRIEEALNRNNHFTPFQLVDTLLSGQAKRAQRILRQLESEGT
EVIILLRTLQKELLLLQTFQYSLSQGEAMFTVFDRYKVWQNRRALYQAALQRLSITQIKQLIRLLAQIEIATKTDYDSDC
WPQLSQLTLDMCNIHISVTSNVSH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=333, Percent_Identity=49.2492492492492, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39671; Mature: 39671

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFPEQLAQNLERGLRQCYLLFGNEPLLKQESLDAIRQAAFTQGFEEKHQFTLDKQLDW
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
NNVFDCTQALSLFSSRQIIELTIPDTGMTTAHTSKLKELVPTLHDDIILLLQGPRVNKAQ
CHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
ENTQWFKALTQLGLFIPCNTPDNQQLPRFIQQRCKKLKLKPDAEAIQMLAQWHEGNLLAL
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
AQSLDKLALLYPDGLLTLIRIEEALNRNNHFTPFQLVDTLLSGQAKRAQRILRQLESEGT
HHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
EVIILLRTLQKELLLLQTFQYSLSQGEAMFTVFDRYKVWQNRRALYQAALQRLSITQIKQ
EEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRLLAQIEIATKTDYDSDCWPQLSQLTLDMCNIHISVTSNVSH
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCC
>Mature Secondary Structure
MRVFPEQLAQNLERGLRQCYLLFGNEPLLKQESLDAIRQAAFTQGFEEKHQFTLDKQLDW
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
NNVFDCTQALSLFSSRQIIELTIPDTGMTTAHTSKLKELVPTLHDDIILLLQGPRVNKAQ
CHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
ENTQWFKALTQLGLFIPCNTPDNQQLPRFIQQRCKKLKLKPDAEAIQMLAQWHEGNLLAL
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
AQSLDKLALLYPDGLLTLIRIEEALNRNNHFTPFQLVDTLLSGQAKRAQRILRQLESEGT
HHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
EVIILLRTLQKELLLLQTFQYSLSQGEAMFTVFDRYKVWQNRRALYQAALQRLSITQIKQ
EEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRLLAQIEIATKTDYDSDCWPQLSQLTLDMCNIHISVTSNVSH
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]