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The map label for this gene is dacA [H]

Identifier: 59711352

GI number: 59711352

Start: 812045

End: 813220

Strand: Reverse

Name: dacA [H]

Synonym: VF_0745

Alternate gene names: 59711352

Gene position: 813220-812045 (Counterclockwise)

Preceding gene: 59711353

Following gene: 59711351

Centisome position: 28.07

GC content: 38.78

Gene sequence:

>1176_bases
ATGAAAAATTCAGCGAAACAGTTAAAACGACTTATCTTATCGAGCTTTATTTTAGGCTCAACCGTTGCTGTAGCAGCACC
TGCGGTTATTCCAAATGCACCGCAAATCGCAGCTAAAGGCTACGTATTAATGGACTATCATTCAGGCAAAGTATTGGCTG
AAAAAGATATGAACGTTCGTATGAACCCTGCAAGCTTAACTAAAATGATGACAAGTTATGTTATCGGTCAAGAAATTGAA
AGTGGCAACATTTCAATGGATGACGATGTTGTTATCAGCAAAAATGCTTGGGCTAAAAACTTCCCTGGTTCATCAAAAAT
GTTCATCGAAGTGGGTAGTACTGTAAAAGTTCGTGAACTTAATAAAGGTATTATCATTCAATCAGGTAACGATGCTTGTG
TTGCTATGGCTGAACACGTAGCCGGCTCTGAAGATGCATTCGTTGACCTAATGAACGCGTGGGCAAAAACATTAAATCTG
AAAAACACACACTTTGCTAACGTTCATGGTCTTGATCGTGATGACCTATACTCAACGCCTTATGATATGGCATTGATTGG
TCAAGCATTAATCCGAGATGTACCTGAAGAATATCGCGTATATTCTGAAAAATCATTCACTTACAACGGTATCACTCAAT
ACAACCGAAATGGTCTATTGTGGGATAAAAGCATGAACGTTGATGGCATTAAAACAGGCCATACAAACGGTGCTGGTTTT
AACCTAGTAAGTTCAGCAACTGAAGGCAAAATGCGTTTAGTTGCCGTTGTTATGGGAACTAAATCTGGTAATGCGCGTAA
AGCTGAAAGTAAAAAGTTACTGAATTACGGTTTCCGTTTCTTTGAAACTGTTTCTCCACATAAAGCTAATGAAACATTCG
TAACTGAAAAAGTATGGATGGGTAGCACTGACGAAGTTGCTTTAGGTGTTGCAGAAGACACGTATGTTACTTTACCACGT
GGCCAAGCTAAGAAAATGACAGCAAGCTTTGTATTAGAAAAAACGCTTGAAGCACCTATCTACAAAGGTGATGTTGTTGG
TAAGCTTTACTACCAAATCGATGGTGAAGACATTGCTGAATACCCACTATTAGCTCTAAATGATGTTGAAGAAGGTGGCA
TCTTTAGCCGTCTTATCGATTACATTGTTCTAATGTTTAAAGGCTGGTTAAGCTAA

Upstream 100 bases:

>100_bases
TTTCATATAAAATCAGTTATTTAATTACACATCTGGTTATGATATTCAAATCTGCTATTATGTACGCAGTTTCACTACCT
CACAGTGCAAAGTTATTATT

Downstream 100 bases:

>100_bases
TATTCAGCTAAACTTAAAACACATAAAAGGCAGCTTCGGTTGCCTTTTTTAATTTACTGGATTAACACTTGTTATCTGAC
CTAGGTCACAGTAATATTCT

Product: D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5)

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKNSAKQLKRLILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVRMNPASLTKMMTSYVIGQEIE
SGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSTVKVRELNKGIIIQSGNDACVAMAEHVAGSEDAFVDLMNAWAKTLNL
KNTHFANVHGLDRDDLYSTPYDMALIGQALIRDVPEEYRVYSEKSFTYNGITQYNRNGLLWDKSMNVDGIKTGHTNGAGF
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWMGSTDEVALGVAEDTYVTLPR
GQAKKMTASFVLEKTLEAPIYKGDVVGKLYYQIDGEDIAEYPLLALNDVEEGGIFSRLIDYIVLMFKGWLS

Sequences:

>Translated_391_residues
MKNSAKQLKRLILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVRMNPASLTKMMTSYVIGQEIE
SGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSTVKVRELNKGIIIQSGNDACVAMAEHVAGSEDAFVDLMNAWAKTLNL
KNTHFANVHGLDRDDLYSTPYDMALIGQALIRDVPEEYRVYSEKSFTYNGITQYNRNGLLWDKSMNVDGIKTGHTNGAGF
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWMGSTDEVALGVAEDTYVTLPR
GQAKKMTASFVLEKTLEAPIYKGDVVGKLYYQIDGEDIAEYPLLALNDVEEGGIFSRLIDYIVLMFKGWLS
>Mature_391_residues
MKNSAKQLKRLILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVRMNPASLTKMMTSYVIGQEIE
SGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSTVKVRELNKGIIIQSGNDACVAMAEHVAGSEDAFVDLMNAWAKTLNL
KNTHFANVHGLDRDDLYSTPYDMALIGQALIRDVPEEYRVYSEKSFTYNGITQYNRNGLLWDKSMNVDGIKTGHTNGAGF
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWMGSTDEVALGVAEDTYVTLPR
GQAKKMTASFVLEKTLEAPIYKGDVVGKLYYQIDGEDIAEYPLLALNDVEEGGIFSRLIDYIVLMFKGWLS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=397, Percent_Identity=55.1637279596977, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1787062, Length=379, Percent_Identity=52.2427440633245, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI87082022, Length=382, Percent_Identity=45.5497382198953, Blast_Score=350, Evalue=7e-98,
Organism=Escherichia coli, GI87082054, Length=237, Percent_Identity=26.5822784810127, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43015; Mature: 43015

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSAKQLKRLILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVR
CCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHCCEEEEEECCCCEEEECCCCEE
MNPASLTKMMTSYVIGQEIESGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSTVKVREL
ECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEC
NKGIIIQSGNDACVAMAEHVAGSEDAFVDLMNAWAKTLNLKNTHFANVHGLDRDDLYSTP
CCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCC
YDMALIGQALIRDVPEEYRVYSEKSFTYNGITQYNRNGLLWDKSMNVDGIKTGHTNGAGF
CHHHHHHHHHHHHCCHHHHHHCCCCCEECCCEEECCCCEEEECCCCCCCEECCCCCCCCC
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWM
EEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GSTDEVALGVAEDTYVTLPRGQAKKMTASFVLEKTLEAPIYKGDVVGKLYYQIDGEDIAE
CCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCHHC
YPLLALNDVEEGGIFSRLIDYIVLMFKGWLS
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNSAKQLKRLILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVR
CCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHCCEEEEEECCCCEEEECCCCEE
MNPASLTKMMTSYVIGQEIESGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSTVKVREL
ECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEC
NKGIIIQSGNDACVAMAEHVAGSEDAFVDLMNAWAKTLNLKNTHFANVHGLDRDDLYSTP
CCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCC
YDMALIGQALIRDVPEEYRVYSEKSFTYNGITQYNRNGLLWDKSMNVDGIKTGHTNGAGF
CHHHHHHHHHHHHCCHHHHHHCCCCCEECCCEEECCCCEEEECCCCCCCEECCCCCCCCC
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWM
EEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GSTDEVALGVAEDTYVTLPRGQAKKMTASFVLEKTLEAPIYKGDVVGKLYYQIDGEDIAE
CCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCHHC
YPLLALNDVEEGGIFSRLIDYIVLMFKGWLS
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]