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The map label for this gene is frmA [H]

Identifier: 59711252

GI number: 59711252

Start: 704236

End: 705384

Strand: Reverse

Name: frmA [H]

Synonym: VF_0645

Alternate gene names: 59711252

Gene position: 705384-704236 (Counterclockwise)

Preceding gene: 59711254

Following gene: 59711251

Centisome position: 24.34

GC content: 44.21

Gene sequence:

>1149_bases
ATGGCGCTTGATATTAAACCTGGTCAAACTCACATTAAATCTAAAGCAATGGTAGCTTGGGCTGCTGGCGAACCTCTTAA
AATGGAAGAAGTGGATGTTCAACTTCCTAAAGCGGGTGAAGTTTTAGTTCGCATCGTTGCTACTGGTGTTTGTCACACTG
ACCTATTCACTCTATCTGGTGATGATCCAGAAGGTATCTTCCCTTCAATCTTAGGTCACGAAGGTGGTGGTATTGTTGAA
ATGGTGGGTGAAGGCGTAACTGGTCTTGAAGTCGGTGACCACGTTATCCCTCTTTACACTGCTGAATGTGGTGAGTGTAA
ATTCTGTAAATCTGGCAAAACTAACCTTTGCCAAGCGGTTCGTGAAACTCAAGGCCAAGGCCTTATGCCTGATGGGACTA
GCCGTTTCTCTATTAACGGTGAAACTATCTTCCATTACATGGGTTGTTCTACGTTCTCTGAATACACAGTTCTTCCTGAA
ATCTCTTTAGCGAAAGTATCTAAAGATGCCCCTCTTGAAGAAGTTTGTCTTCTAGGTTGTGGTGTTACTACTGGTATGGG
TGCGGTTCTTAACACTGCTAAAGTTGAAAAAGGCGACACAGTTGCTGTATTCGGTCTAGGTGGTATCGGTCTTTCAGCTA
TCATCGGTGCAAAAATGGCTGGTGCAAGCCGCATCATCGGTGTTGATTTGAACGAAAGCAAATACGAACTGGCTAAAAAA
TTAGGTGCAACTGATTGTATCAACCCTATGAACTACGACAAGCCAATTCAAGAAGTGATTGTTGAAATGACTGACGGTGG
TGTTGATTACTCTTTCGAGTGTATCGGTAACGTTAACGTTATGCGTTCAGCTCTTGAGTGTTGTCACAAAGGTTGGGGTG
AATCAATCATCATCGGTGTTGCTGGTGCAGGTCAAGAAATCTCAACTCGTCCGTTCCAACTAGTAACGGGTCGTGTATGG
CGTGGCTCTGCATTCGGTGGCGTTAAAGGCCGTACTGAACTTCCAGGTATCGTTGACCGTTACATGCAAGGTGAGTTCGC
TCTTGATGACTTCATCACTCACACTATGGGTCTTGCGGATGTAAACGCTGCGTTCGATCTAATGCACGAAGGTAAATCTA
TCCGTACTGTTCTACATATGGATAAATAA

Upstream 100 bases:

>100_bases
TCACACTTATACTAGCCACATCAAAACGAGAGAGCAGAAATAAAAAACTCAACTACGCTTTAGTTGACTGCTCATCCGAA
TATATTAAAAGGAAAATCCA

Downstream 100 bases:

>100_bases
GTAAAAGTAAGGGGTTAGATACTCTAGCCCCTATTCTACTTTTGATCTCCAAGTGCTTTATATAAAGACAGTATGCTTAT
CTGCGTGGGTGGCGCAATTT

Product: alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MALDIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDLFTLSGDDPEGIFPSILGHEGGGIVE
MVGEGVTGLEVGDHVIPLYTAECGECKFCKSGKTNLCQAVRETQGQGLMPDGTSRFSINGETIFHYMGCSTFSEYTVLPE
ISLAKVSKDAPLEEVCLLGCGVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGAKMAGASRIIGVDLNESKYELAKK
LGATDCINPMNYDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESIIIGVAGAGQEISTRPFQLVTGRVW
RGSAFGGVKGRTELPGIVDRYMQGEFALDDFITHTMGLADVNAAFDLMHEGKSIRTVLHMDK

Sequences:

>Translated_382_residues
MALDIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDLFTLSGDDPEGIFPSILGHEGGGIVE
MVGEGVTGLEVGDHVIPLYTAECGECKFCKSGKTNLCQAVRETQGQGLMPDGTSRFSINGETIFHYMGCSTFSEYTVLPE
ISLAKVSKDAPLEEVCLLGCGVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGAKMAGASRIIGVDLNESKYELAKK
LGATDCINPMNYDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESIIIGVAGAGQEISTRPFQLVTGRVW
RGSAFGGVKGRTELPGIVDRYMQGEFALDDFITHTMGLADVNAAFDLMHEGKSIRTVLHMDK
>Mature_381_residues
ALDIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDLFTLSGDDPEGIFPSILGHEGGGIVEM
VGEGVTGLEVGDHVIPLYTAECGECKFCKSGKTNLCQAVRETQGQGLMPDGTSRFSINGETIFHYMGCSTFSEYTVLPEI
SLAKVSKDAPLEEVCLLGCGVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGAKMAGASRIIGVDLNESKYELAKKL
GATDCINPMNYDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESIIIGVAGAGQEISTRPFQLVTGRVWR
GSAFGGVKGRTELPGIVDRYMQGEFALDDFITHTMGLADVNAAFDLMHEGKSIRTVLHMDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=65.0406504065041, Blast_Score=504, Evalue=1e-143,
Organism=Homo sapiens, GI34577061, Length=367, Percent_Identity=51.7711171662125, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4501929, Length=367, Percent_Identity=51.4986376021798, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI71565152, Length=371, Percent_Identity=50.9433962264151, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI4501933, Length=367, Percent_Identity=50.408719346049, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=47.9674796747967, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=48.1994459833795, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI262073058, Length=368, Percent_Identity=47.2826086956522, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI71743840, Length=368, Percent_Identity=47.2826086956522, Blast_Score=358, Evalue=6e-99,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=68.6648501362398, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1790045, Length=372, Percent_Identity=28.494623655914, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082125, Length=366, Percent_Identity=25.6830601092896, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787863, Length=350, Percent_Identity=25.4285714285714, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788073, Length=348, Percent_Identity=26.7241379310345, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.9473684210526, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786825, Length=267, Percent_Identity=29.5880149812734, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788075, Length=367, Percent_Identity=25.3405994550409, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI226510992, Length=186, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786518, Length=250, Percent_Identity=28.4, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=375, Percent_Identity=61.3333333333333, Blast_Score=490, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71997431, Length=377, Percent_Identity=61.0079575596817, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17565904, Length=377, Percent_Identity=57.2944297082228, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17562584, Length=371, Percent_Identity=29.3800539083558, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=289, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562878, Length=258, Percent_Identity=29.4573643410853, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=363, Percent_Identity=27.5482093663912, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=363, Percent_Identity=26.1707988980716, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=59.7333333333333, Blast_Score=474, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6319621, Length=378, Percent_Identity=30.1587301587302, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323729, Length=365, Percent_Identity=29.3150684931507, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324486, Length=375, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=27.1978021978022, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323099, Length=376, Percent_Identity=28.4574468085106, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319258, Length=276, Percent_Identity=27.536231884058, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322619, Length=356, Percent_Identity=26.9662921348315, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319955, Length=356, Percent_Identity=26.9662921348315, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=277, Percent_Identity=25.9927797833935, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17737895, Length=369, Percent_Identity=62.3306233062331, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17137530, Length=268, Percent_Identity=30.2238805970149, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17737897, Length=268, Percent_Identity=29.1044776119403, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40546; Mature: 40415

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDLFTLSG
CEECCCCCCHHHCCCEEEEEECCCCCEEECCEEECCCHHHHHHHHHHHCCHHHEEEEECC
DDPEGIFPSILGHEGGGIVEMVGEGVTGLEVGDHVIPLYTAECGECKFCKSGKTNLCQAV
CCCCCCCHHHHCCCCCCEEEECCCCCCCEECCCEEEEEEECCCCCEECCCCCCHHHHHHH
RETQGQGLMPDGTSRFSINGETIFHYMGCSTFSEYTVLPEISLAKVSKDAPLEEVCLLGC
HHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHC
GVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGAKMAGASRIIGVDLNESKYELAKK
CHHHCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
LGATDCINPMNYDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESIIIGV
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEE
AGAGQEISTRPFQLVTGRVWRGSAFGGVKGRTELPGIVDRYMQGEFALDDFITHTMGLAD
ECCCCCCCCCCEEEEECEEECCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCHHH
VNAAFDLMHEGKSIRTVLHMDK
HHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
ALDIKPGQTHIKSKAMVAWAAGEPLKMEEVDVQLPKAGEVLVRIVATGVCHTDLFTLSG
EECCCCCCHHHCCCEEEEEECCCCCEEECCEEECCCHHHHHHHHHHHCCHHHEEEEECC
DDPEGIFPSILGHEGGGIVEMVGEGVTGLEVGDHVIPLYTAECGECKFCKSGKTNLCQAV
CCCCCCCHHHHCCCCCCEEEECCCCCCCEECCCEEEEEEECCCCCEECCCCCCHHHHHHH
RETQGQGLMPDGTSRFSINGETIFHYMGCSTFSEYTVLPEISLAKVSKDAPLEEVCLLGC
HHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHC
GVTTGMGAVLNTAKVEKGDTVAVFGLGGIGLSAIIGAKMAGASRIIGVDLNESKYELAKK
CHHHCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
LGATDCINPMNYDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESIIIGV
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEE
AGAGQEISTRPFQLVTGRVWRGSAFGGVKGRTELPGIVDRYMQGEFALDDFITHTMGLAD
ECCCCCCCCCCEEEEECEEECCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCHHH
VNAAFDLMHEGKSIRTVLHMDK
HHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]