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The map label for this gene is nfrA

Identifier: 57652640

GI number: 57652640

Start: 454393

End: 455148

Strand: Direct

Name: nfrA

Synonym: SACOL0453

Alternate gene names: 57652640

Gene position: 454393-455148 (Clockwise)

Preceding gene: 57652634

Following gene: 57652645

Centisome position: 16.17

GC content: 38.1

Gene sequence:

>756_bases
GTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGT
TGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTATTGGTATCGACG
ATGAGAAGATTAAAGAAAATTTACGAGAAGTTTCTGGACAACCTTATGTTGTAGAAAATGGCTATTTATTCGTCTTTGTT
ATTGATTATTATCGTCATCATTTAGTTGATCAACATGCTGAAACTGATATGGAAAATGCATATGGTTCAACGGAAGGTTT
GCTAGTAGGTGCAATCGATGCAGCATTAGTTGCCGAAAATATTGCGGTAACTGCTGAAGATATGGGGTATGGCATTGTCT
TTTTAGGATCATTAAGAAATGATGTTGAACGCGTTCGAGAAATTTTAGACTTACCTGACTATGTCTTCCCGGTATTTGGT
ATGGCAGTAGGGGAACCCGCAGATGACGAAAATGGTGCAGCCAAGCCACGCTTACCATTTGACCATGTCTTCCATCATAA
TAAGTATCATGCTGATAAGGAAACACAGTATGCACAAATGGCAGATTACGACCAGACAATCAGCGAGTACTATGATCAAC
GTACAAACGGGAATCGCAAAGAAACATGGTCGCAGCAAATTGAGATGTTCCTAGGAAACAAAGCAAGATTAGATATGTTA
GAACAATTGCAAAAATCAGGCTTAATACAGCGATAG

Upstream 100 bases:

>100_bases
ATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAA
AGTATCGAGGAGTGGGTAAC

Downstream 100 bases:

>100_bases
CAAGATACCAAAATAACCCGCCCCCCTCTAGCTTAAAATGATAAGTATAGCTAGAGGGGGCGGGTATTTCTTGCAATGAA
TTAGTGTGAAGTTAATGCAG

Product: NAD(P)H-flavin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFV
IDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
EQLQKSGLIQR

Sequences:

>Translated_251_residues
MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFV
IDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
EQLQKSGLIQR
>Mature_250_residues
SEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVI
DYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFGM
AVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDMLE
QLQKSGLIQR

Specific function: Reduces FMN, organic nitro compounds and disulfide DTNB. Involved in maintenance of the cellular redox state and the disulfide stress response

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family

Homologues:

Organism=Escherichia coli, GI1787075, Length=246, Percent_Identity=36.5853658536585, Blast_Score=154, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFRA_STAA3 (Q2FJN3)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_493095.1
- ProteinModelPortal:   Q2FJN3
- SMR:   Q2FJN3
- STRING:   Q2FJN3
- EnsemblBacteria:   EBSTAT00000038427
- GeneID:   3915054
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_0381
- eggNOG:   COG0778
- GeneTree:   EBGT00050000025218
- HOGENOM:   HBG532063
- OMA:   HENGYDE
- ProtClustDB:   CLSK884604
- InterPro:   IPR016446
- InterPro:   IPR000415
- PIRSF:   PIRSF005426

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28597; Mature: 28466

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKEN
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
LREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAEN
HHHHCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
IAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFGMAVGEPADDENGAAKPRLPF
HEEEHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCH
DHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
EQLQKSGLIQR
HHHHHCCCCCC
>Mature Secondary Structure 
SEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFLQAYSIIGIDDEKIKEN
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
LREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYGSTEGLLVGAIDAALVAEN
HHHHCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
IAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFGMAVGEPADDENGAAKPRLPF
HEEEHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCH
DHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGNRKETWSQQIEMFLGNKARLDML
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
EQLQKSGLIQR
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA