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The map label for this gene is icaA

Identifier: 57652406

GI number: 57652406

Start: 2763320

End: 2764558

Strand: Direct

Name: icaA

Synonym: SACOL2689

Alternate gene names: 57652406

Gene position: 2763320-2764558 (Clockwise)

Preceding gene: 57652398

Following gene: 57651060

Centisome position: 98.36

GC content: 34.38

Gene sequence:

>1239_bases
TTGCAATTTTTTAACTTTTTGCTTTTTTATCCTGTATTTATGTCTATTTACTGGATTGTCGGTTCAATTTATTTCTATTT
TACCAGAGAAATTAGATATTCATTGAACAAGAAGCCTGACATAAATGTGGATGAATTAGAAGGCATTACATTTTTACTTG
CCTGTTATAACGAAAGTGAAACGATTGAAGATACGTTGTCTAATGTTCTTGCACTCAAATACGAGAAGAAAGAAATTATT
ATCATTAATGATGGAAGTTCAGATAATACAGCAGAACTCATCTATAAAATCAAAGAAAATAATGACTTTATTTTCGTCGA
TTTACAAGAAAACAGAGGTAAAGCCAACGCACTCAATCAAGGCATTAAACAGGCTTCATATGATTATGTAATGTGCTTGG
ATGCAGATACTATCGTTGATCAAGATGCACCATATTATATGATTGAGAATTTCAAACATGATCCAAAACTTGGTGCAGTT
ACAGGTAATCCTAGAATTCGAAATAAGAGTTCTATTTTAGGTAAAATTCAAACGATAGAATATGCAAGTTTAATTGGCTG
TATTAAGCGAAGTCAGACACTTGCTGGCGCAGTCAATACTATTTCGGGTGTCTTCACTCTATTTAAAAAAAGTGCAGTTG
TCGACGTTGGCTACTGGGATACTGATATGATTACCGAAGATATTGCAGTTTCTTGGAAATTGCATTTACGTGGATATCGT
ATTAAGTATGAACCGCTTGCCATGTGTTGGATGTTGGTTCCAGAAACATTGGGAGGTCTTTGGAAGCAACGCGTGAGATG
GGCTCAAGGGGGACACGAAGTATTACTACGAGACTTTTTTAGCACAATGAAAACGAAAAGGTTTCCTTTATATATTTTGA
TGTTTGAGCAAATCATCTCGATTTTATGGGTATATATAGTGCTTCTATATTTAGGCTATTTGTTCATAACAGCAAACTTC
TTAGACTATACATTTATGACATATAGTTTTTCAATATTTCTACTATCATCATTTACTATGACTTTTATAAACGTTATTCA
ATTTACAGTCGCACTCTTTATTGATAGTCGCTACGAGAAAAAGAATATGGCTGGACTCATATTTGTAAGTTGGTATCCGA
CAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCA
ACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAA

Upstream 100 bases:

>100_bases
AAATATTTCCGTTTAATTATAACAACAATCTATTGCAAATTAAAATACTATCAATTACCATATGGCTTACAACCTAACTA
ACGAAAGGTAGGTAAAGAAA

Downstream 100 bases:

>100_bases
AATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTA
CTCGTTTATATTGGTACTAT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEII
IINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANF
LDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYA
TWSSPDRGNTQR

Sequences:

>Translated_412_residues
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEII
IINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANF
LDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYA
TWSSPDRGNTQR
>Mature_412_residues
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEII
IINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANF
LDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYA
TWSSPDRGNTQR

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family

Homologues:

Organism=Escherichia coli, GI1787259, Length=414, Percent_Identity=39.6135265700483, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1786560, Length=215, Percent_Identity=32.093023255814, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ICAA_STAA8 (Q9RQP9)

Other databases:

- EMBL:   AF086783
- EMBL:   CP000253
- RefSeq:   YP_501451.1
- ProteinModelPortal:   Q9RQP9
- STRING:   Q9RQP9
- EnsemblBacteria:   EBSTAT00000028991
- GeneID:   3921484
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_03002
- eggNOG:   COG1215
- GeneTree:   EBGT00050000024002
- HOGENOM:   HBG514801
- OMA:   AWAYSMS
- ProtClustDB:   PRK11204
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: NA

Molecular weight: Translated: 47770; Mature: 47770

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x108cd900)-; HASH(0x10d81ae4)-; HASH(0x10616f0c)-; HASH(0x10d24b34)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCC
TIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQ
HHHHHHHHHHEEEECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHH
GIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIE
HHHHCCCCEEEEECCHHHCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
YASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEEEEEEEEEE
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIIS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCC
TIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQ
HHHHHHHHHHEEEECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHH
GIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIE
HHHHCCCCEEEEECCHHHCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
YASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEEEEEEEEEE
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIIS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925