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The map label for this gene is ribBA

Identifier: 57652065

GI number: 57652065

Start: 1873086

End: 1874267

Strand: Reverse

Name: ribBA

Synonym: SACOL1818

Alternate gene names: 57652065

Gene position: 1874267-1873086 (Counterclockwise)

Preceding gene: 57652066

Following gene: 57652064

Centisome position: 66.71

GC content: 34.09

Gene sequence:

>1182_bases
ATGCAATTCGATAATATTGACAGTGCTTTAATGGCTTTAAAAAATGGAGAACCAATTATTGTAGTAGATGATGAGAATCG
TGAAAATGAAGGTGATTTAGTAGCGGTTACTGAATGGATGAACGATAATACCATTAATTTTATGGCGAAAGAAGCAAGGG
GATTAATATGCGCACCAGTGTCTAAAGATATTGCACAACGTTTGGATTTGGTACAAATGGTTGATGATAACTCCGACATC
TTTGGTACGCAATTTACAGTGAGTATTGATCATGTAGATACAACAACAGGAATTAGTGCTTATGAACGTACATTGACTGC
CAAAAAGCTCATTGATCCTAGTAGTGAAGCTAAAGATTTTAATCGTCCTGGTCATTTATTTCCATTAGTAGCACAAGATA
AAGGCGTATTAGCTAGAAATGGACACACAGAAGCGGCTGTTGATTTAGCTAAACTTACTGGTGCCAAGCCCGCTGGTGTC
ATTTGTGAGATTATGAATGATGACGGCACGATGGCGAAAGGACAAGATTTACAAAAGTTTAAAGAAAAACATCAATTAAA
GATGATTACGATTGATGATTTAATTGAATATCGTAAAAAATTAGAACCAGAAATTGAATTTAAGGCAAAAGTGAAAATGC
CTACAGATTTCGGAACATTTGATATGTATGGTTTTAAAGCGACATACACAGATGAAGAGATAGTTGTACTGACAAAAGGT
GCAATTCGACAACATGAAAATGTACGCTTACATTCTGCGTGCCTTACAGGCGATATTTTCCATAGTCAACGTTGTGATTG
TGGTGCTCAACTTGAATCGTCTATGAAGTATATCAATGAACATGGTGGCATGATTATTTATCTACCTCAAGAAGGTCGTG
GCATAGGATTGTTAAACAAATTACGCGCATATGAATTAATTGAGCAAGGATATGATACAGTAACTGCAAATTTAGCATTA
GGTTTTGATGAAGATTTGCGAGATTATCATATTGCTGCACAGATTTTAAAATATTTTAACATCGAACATATCAATTTATT
AAGTAATAATCCAAGTAAATTTGAGGGATTAAAACAATATGGCATTGATATTGCAGAAAGAATTGAAGTTATCGTACCAG
AAACGGTACATAATCATGATTATATGGAAACGAAAAAAATAAAAATGGGTCATTTAATATAG

Upstream 100 bases:

>100_bases
ACAGACGTTTTGTTTAAATATGTTGAAAATATTTTAAATAAAGATAAAGACCAATTATCTGTAGATAAATTAAGAGCATT
TGGTTTTTAGGAGGGGTAGC

Downstream 100 bases:

>100_bases
GAGGATTTTAACATGAATTTTGAAGGTAAATTAATTGGAAAAGATTTGAAAGTTGCAATCGTAGTTAGTCGATTTAATGA
TTTTATCACTGGAAGATTAC

Product: riboflavin biosynthesis protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDI
FGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLAL
GFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHLI

Sequences:

>Translated_393_residues
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDI
FGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLAL
GFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHLI
>Mature_393_residues
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDI
FGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLAL
GFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHLI

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=44.2786069651741, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787533, Length=188, Percent_Identity=48.936170212766, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=43.4782608695652, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6319438, Length=169, Percent_Identity=37.8698224852071, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_STAAC (Q5HF07)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186650.1
- ProteinModelPortal:   Q5HF07
- STRING:   Q5HF07
- EnsemblBacteria:   EBSTAT00000009545
- GeneID:   3236545
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1818
- TIGR:   SACOL1818
- eggNOG:   COG0108
- GeneTree:   EBGT00050000025480
- HOGENOM:   HBG735778
- OMA:   RCDCRMQ
- ProtClustDB:   CLSK885544
- BioCyc:   SAUR93062:SACOL1818-MONOMER
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 44189; Mature: 44189

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: ACT_SITE 325-325 ACT_SITE 327-327 BINDING 32-32 BINDING 163-163 BINDING 270-270 BINDING 313-313 BINDING 348-348 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPV
CCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEHHCCCEEEECC
SKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDF
CHHHHHHHHHHHHHCCCCCEECEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
NRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKF
CCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
KEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
HHHHCEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCEEEECCCCEEEEEECC
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNK
CCCCCCCCEEEHHEEECCHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
LRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQY
HHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
GIDIAERIEVIVPETVHNHDYMETKKIKMGHLI
CCCHHHHEEEEECCCCCCCCCCHHHCEECCCCC
>Mature Secondary Structure
MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPV
CCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEHHCCCEEEECC
SKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDF
CHHHHHHHHHHHHHCCCCCEECEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
NRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKF
CCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
KEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKG
HHHHCEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCEEEECCCCEEEEEECC
AIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNK
CCCCCCCCEEEHHEEECCHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
LRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQY
HHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
GIDIAERIEVIVPETVHNHDYMETKKIKMGHLI
CCCHHHHEEEEECCCCCCCCCCHHHCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA