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The map label for this gene is greA

Identifier: 57652001

GI number: 57652001

Start: 1693885

End: 1694361

Strand: Reverse

Name: greA

Synonym: SACOL1665

Alternate gene names: 57652001

Gene position: 1694361-1693885 (Counterclockwise)

Preceding gene: 57652002

Following gene: 57652000

Centisome position: 60.31

GC content: 33.33

Gene sequence:

>477_bases
ATGGAAAATCAAAAGCAATATCCAATGACTCAAGAAGGTTTTGAAAAATTAGAGCGTGAACTTGAAGAATTAAAAACAGT
TAAGCGTCCTGAAGTTGTAGAGAAAATTAAAGTTGCACGTTCATTTGGTGACTTATCAGAGAACTCTGAGTATGATGCAG
CAAAAGATGAACAAGGATTCATCGAACAAGATATTCAAAGAATTGAGCATATGTTAAGAAATGCATTAATCATTGAAGAT
ACTGGAGATAACAACGTTGTTAAAATTGGTAAAACAGTAACGTTTGTAGAATTACCAGGTGATGAAGAGGAAAGTTATCA
AATCGTTGGTTCAGCTGAATCAGATGCATTTAATGGTAAGATTTCAAATGAATCACCAATGGCTAAAGCGTTAATTGGTA
AAGGTTTAGATGATGAAGTTCGTGTTCCACTACCTAATGGTGGCGAAATGAACGTAAAAATTGTTAATATCCAATAA

Upstream 100 bases:

>100_bases
TGAAGGTGGGAGCAATAAAGTTGCAATAGATATTATGACAACAAAAATTCAGTCTTTAGTTAGCAAGCAATAGTATAATT
CAGTAAAGGAAGATGACAAT

Downstream 100 bases:

>100_bases
ATGATCAAATAGATTATTTAATTGCGCGATTGAAGTGTGTTAATGATACTTCGGTCGTGCTTTTTTATATTCAAAATCAA
AAAATCGGACAGATGAAAAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGDLSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIED
TGDNNVVKIGKTVTFVELPGDEEESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ

Sequences:

>Translated_158_residues
MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGDLSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIED
TGDNNVVKIGKTVTFVELPGDEEESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ
>Mature_158_residues
MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGDLSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIED
TGDNNVVKIGKTVTFVELPGDEEESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=46.4052287581699, Blast_Score=122, Evalue=9e-30,
Organism=Escherichia coli, GI87082261, Length=143, Percent_Identity=34.965034965035, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_STAA1 (A7X319)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442187.1
- ProteinModelPortal:   A7X319
- SMR:   A7X319
- STRING:   A7X319
- EnsemblBacteria:   EBSTAT00000003906
- GeneID:   5561479
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1597
- eggNOG:   COG0782
- GeneTree:   EBGT00050000025035
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   SAUR418127:SAHV_1597-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17743; Mature: 17743

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGDLSENSEYDAAKDEQGF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
IEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDEEESYQIVGSAESDAFNGK
HHHHHHHHHHHHHCEEEEEECCCCCEEEECCEEEEEECCCCCCCCEEEEECCCCCCCCCC
ISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ
CCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEEC
>Mature Secondary Structure
MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGDLSENSEYDAAKDEQGF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
IEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDEEESYQIVGSAESDAFNGK
HHHHHHHHHHHHHCEEEEEECCCCCEEEECCEEEEEECCCCCCCCEEEEECCCCCCCCCC
ISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ
CCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA