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The map label for this gene is queF

Identifier: 57651565

GI number: 57651565

Start: 813187

End: 813687

Strand: Reverse

Name: queF

Synonym: SACOL0789

Alternate gene names: 57651565

Gene position: 813687-813187 (Counterclockwise)

Preceding gene: 57651566

Following gene: 57651564

Centisome position: 28.96

GC content: 31.54

Gene sequence:

>501_bases
ATGGCACATGGCCGTCAACAAGATGAATTACAAGATATAACTTTATTAGGAAATCAAGACAATACTTACAATTTTGACTA
TCGTCCTGATGTATTAGAATCGTTTGATAATAAGCATCAAGGTCGTGACTATTTCGTAAAATTCAACTGTCCAGAATTTA
CATCATTATGTCCAATTACTGGGCAACCAGATTTTGCAACTATCTATATTTCATATATTCCAAATGTTAAAATGGTTGAA
TCAAAATCTTTGAAATTATACTTATTTAGTTTCAGAAATCACGGTGATTTTCACGAAGATTGTATGAATATTATTATGAA
TGATTTGATAGAGCTTATGGACCCACATTATATTGAAGTCTGGGGCAAGTTCACACCACGTGGTGGAATTTCTATTGATC
CTTATACAAACTATGGACGTCCAAATTCTAAATATGAAAAAATGGCTGAGCATCGTTTGATGAATCATGATTTATATCCC
GAAAAAATAGATAATCGTTAA

Upstream 100 bases:

>100_bases
TTAGGTTACGTCGTTATTATCGGTGCAAGTTATTACATGTTTGAAAAAGCACGCCGAGACGCCAAAATAATAAAAAAATA
AATTTAAAGGAGCGCTTTAT

Downstream 100 bases:

>100_bases
ATGTATCATTTAATAAACACACCAATAAGTTGATTTTCCTAACTTATTGGTGTGTTTTTCATTTAGCATACATAATAGGT
TACATTAAAATAACATTTTA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDYFVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVE
SKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
EKIDNR

Sequences:

>Translated_166_residues
MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDYFVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVE
SKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
EKIDNR
>Mature_165_residues
AHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDYFVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVES
KSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYPE
KIDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=123, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_STAA1 (A7WZL5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441315.1
- STRING:   A7WZL5
- EnsemblBacteria:   EBSTAT00000004947
- GeneID:   5561215
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0725
- eggNOG:   COG0780
- GeneTree:   EBGT00050000023888
- HOGENOM:   HBG294103
- OMA:   CINTILL
- ProtClustDB:   PRK13258
- BioCyc:   SAUR418127:SAHV_0725-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 19645; Mature: 19514

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDYFVKFNCPEFTSLCPIT
CCCCCCCCCCCEEEEECCCCCCEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
GQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPHYIEV
CCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEE
WGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYPEKIDNR
EECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
AHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDYFVKFNCPEFTSLCPIT
CCCCCCCCCCEEEEECCCCCCEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
GQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCMNIIMNDLIELMDPHYIEV
CCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEE
WGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYPEKIDNR
EECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA