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The map label for this gene is nirB [H]

Identifier: 57650943

GI number: 57650943

Start: 2458510

End: 2460915

Strand: Reverse

Name: nirB [H]

Synonym: SACOL2398

Alternate gene names: 57650943

Gene position: 2460915-2458510 (Counterclockwise)

Preceding gene: 57650944

Following gene: 57650942

Centisome position: 87.6

GC content: 36.37

Gene sequence:

>2406_bases
ATGGCAAAGCAAAAACTAGTAATGATTGGTAACGGTATGGCGGGCATTCGAACAATCGAAGAAATATTAGAGCGCGCCAA
CGATTTATATGATATTACGGTAATAGGTAAAGAACCTTATCCAAACTATAACCGCATCATGCTTTCAAATATTTTACAAA
ATAAAATGACAGTTGAAGAAACAATTATGAATCCATATGAGTGGTATGAGGAACATGGTATCGAGTTAATTACAAATGAT
CCTGTAATTGAAGTAGATAGAGCAAATCAATCAGTTACTACTGCAAATGGTATTGAAGTATCATACGATAAATTGATTTT
TGCGACAGGTTCTAAAGCATTCGTCATTCCAGTTCCAGGTTCGACATTACCGAGTGTAATTGGTTGGAGAACGATTGATG
ATACTGAACAAATGATGAACATTGCTAAGACTAAAAAGAAAGCAATTGTAATTGGTGGAGGATTACTAGGTTTAGAGTGT
GCACGTGGTTTATTAGATCAAGGTATGGAAGTAACGGTGTTACATTTAGCTGAATGGTTGATGGAAATGCAACTAGACCG
TAAAGCTGGAAATATGCTTAAAGCAGATCTAGAAAAGCAAGGTATGAAGTTTGAAATGCAAGCCAACACAACTGAAATCT
TAGGAGAAGATGATGTTGAGGGTGTTAAATTGGCTGATGGACGCGAGATTCCGGCAGACTTAGTTGTTATGGCAGTAGGT
ATACGACCATACACAGAAGTAGCCAAAGAATCAGGTCTAGATGTTAATCGTGGTATTGTTGTCAATGATGTGATGCAAAC
AAGTGATAGCAATGTATATGCAGTTGGTGAATGTGCAGAACATAACGGCAAAGTTTATGGACTCGTTGCACCACTATATG
AACAAGGTAAAGTATTAGCTGATCATTTAACAAATAAAGAAACGAACGGATACAAGGGATCAACAACATTTACGTCATTA
AAAGTTTCTGGGTGTGACTTGTATAGTGCTGGTCAAATTGTAGAAAATGCAGAAATTAAAGGTATTGAAATATTTAATAG
TGTTGATAATAACTATAAAAAAATCTTTTTAAAAGACGGTAATGTAGTTGGTGCAGTACTATATGGTGATATCGATGATG
GTTCACGCTTTTATAACATGATGAAAAAAGGTGAATCCACTGAAGATTACACACTTGTATCATTGCTTACTAAAGGTGGA
GAAGAGGCATCGCTATCAATTGCTGATATGGCTGATGATGAAACAATTTGTGGTTGTAATGGTGTTGATAAAGGTACTAT
AGTAAATGCGATTACGGAAAATGGCTTTACAACAGTTGAAGAAGTAACGGCTAAAACAAAAGCGGGGAATTCATGTGGTA
AATGTAAACCGCAAATTGCTCAAATATTGCAGCACACCTTAGGAGATGACTTTGTTGCCGCAAAACCTGCTGGTATATGT
GGTTGTACTGATTTGACACGCGATCAAATAGTAACGCAAATAAGAGCGAAAGGTTTAAAAACATCTAAAGAAGTTCGACA
TGTTTTAAACTTTAAAAATAAAGGTGGATGTCCAAAATGTCGACCAGCAATCAACTATTATTTAAACATGGTTTATCCAC
ATGATCATGAAGATGAAAGAGAATCAAGATTTGCTAACGAACGTTACCATGCGAATATTCAAAATGATGGTACATTTTCT
GTTATACCTCAAATGCGTGGGGGTGTTACAGATGCAGACCAACTGATTCGTCTAGGAGAAGTGGCTAAGAAATATCATGT
GCCACTAGTTAAAGTGACAGGTTCACAACGTGTTGGTTTGTATGGAGTTAAAAAAGAAGAATTACCAAATATATGGGAAG
ACCTAGGTATGCGTTCAGCATCAGCTTATGGTAAGAAAACACGCTCAGTTAAAAGCTGTGTTGGTAAAGAGTTTTGTCGA
TTTGGTACGCAATACACGACACGACTTGGCATTCGTTTAGAAAAAACATTTGAATACATCGATACACCTCATAAATTCAA
AATGGGTGTATCAGGCTGCCCAAGAAGTTGTGTTGAATCAGGTGTTAAAGATTTTGGTATTATCTCAGTTGAAAATGGAT
TCCAAATCTATATCGGTGGTAATGGTGGTACAGAAGTTGAAAAAGCTGAATTTTTAACAACTGTAGAAACAGAAGATGAA
GTAATCAAATTATGTGGTGCTTTGATGCAATATTATCGCGAAACAGGTATATATGCTGAAAGAACAGCGCCATGGTTAAG
AAGACTTGGATTTGAAAATGTAAAAGAAGTCTTACTTGACCCAGAAAGACAAAATGAACTATTTGAACGCATTATGGATG
CTAAAAAAGCAGTCGAAGCTGAACCTTGGGAAGCAATAACTAGCAATGCGCAAGCACGTAGAATTTTTGAAGTGGAGAAG
GTGTAA

Upstream 100 bases:

>100_bases
GATTAGAAAGCTTAATGATTCCAATGAAAATATAAAAGCACTAACGAAAAATTAATACCTTTTTTTGAAATAAATATTAT
GGAGTAAAGGGGCATATTTT

Downstream 100 bases:

>100_bases
TACATGGAAACAAAAGAAAAAATTAAAGTGACAACTATAGATGAATTAACACCCCTAATTGGAAAAAAGGTTATTGTCAA
AGGCAAAGAGGTAGGGTTGT

Product: nitrite reductase [NAD(P)H], large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 800

Protein sequence:

>801_residues
MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITND
PVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSL
KVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGG
EEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFS
VIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCR
FGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEVEK
V

Sequences:

>Translated_801_residues
MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITND
PVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSL
KVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGG
EEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFS
VIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCR
FGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEVEK
V
>Mature_800_residues
AKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYNRIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDP
VIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLECA
RGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVGI
RPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLK
VSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGE
EASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGICG
CTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSV
IPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRF
GTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEV
IKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEVEKV

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=387, Percent_Identity=27.1317829457364, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI21389617, Length=387, Percent_Identity=27.1317829457364, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI226437568, Length=387, Percent_Identity=27.1317829457364, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI22202629, Length=221, Percent_Identity=28.9592760180996, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI4757732, Length=221, Percent_Identity=28.9592760180996, Blast_Score=74, Evalue=8e-13,
Organism=Escherichia coli, GI1789765, Length=819, Percent_Identity=35.5311355311355, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1789065, Length=332, Percent_Identity=28.0120481927711, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788892, Length=251, Percent_Identity=23.1075697211155, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=428, Percent_Identity=27.803738317757, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32565766, Length=220, Percent_Identity=28.6363636363636, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281359715, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359713, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639250, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18543267, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639252, Length=287, Percent_Identity=27.5261324041812, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639257, Length=290, Percent_Identity=27.2413793103448, Blast_Score=115, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585130, Length=281, Percent_Identity=25.2669039145907, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21358499, Length=215, Percent_Identity=30.6976744186047, Blast_Score=81, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28573993, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581020, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88658; Mature: 88527

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYNRIMLSNILQNKMTVEE
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCHHHHH
TIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPG
HHCCHHHHHHHCCCEEEECCCEEEEECCCCCEEECCCCEEEECEEEEEECCCEEEEECCC
STLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLECARGLLDQGMEVTVLHLAEWL
CCCCHHHCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEHHHHHHHHH
MEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
HHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHCCCCCCCCEEECCCCCCCHHHEEEEEC
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLA
CCCHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEEEEHHCCCCEEEEEECCHHHCCCHHH
DHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDG
HHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCCCCEEEEECCCCCCEEEEEEECC
NVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGEEASLSIADMADDETICGCN
CEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEECCC
GVDKGTIVNAITENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDER
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHEEEECCCCCCHHH
ESRFANERYHANIQNDGTFSVIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGL
HHHHCCCCEEECCCCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYI
ECCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHEECEEEEEHHHHH
DTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
CCCCCEECCCCCCCHHHHHCCCHHCEEEEECCCEEEEECCCCCCCCHHHHHEEECCCHHH
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEA
HHHHHHHHHHHHHHCCCCHHHCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
EPWEAITSNAQARRIFEVEKV
CCHHHHHCCCHHHEEEEECCC
>Mature Secondary Structure 
AKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYNRIMLSNILQNKMTVEE
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCHHHHH
TIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSYDKLIFATGSKAFVIPVPG
HHCCHHHHHHHCCCEEEECCCEEEEECCCCCEEECCCCEEEECEEEEEECCCEEEEECCC
STLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLECARGLLDQGMEVTVLHLAEWL
CCCCHHHCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEHHHHHHHHH
MEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDDVEGVKLADGREIPADLVVMAVG
HHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHCCCCCCCCEEECCCCCCCHHHEEEEEC
IRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVGECAEHNGKVYGLVAPLYEQGKVLA
CCCHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEEEEHHCCCCEEEEEECCHHHCCCHHH
DHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIVENAEIKGIEIFNSVDNNYKKIFLKDG
HHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCCCCEEEEECCCCCCEEEEEEECC
NVVGAVLYGDIDDGSRFYNMMKKGESTEDYTLVSLLTKGGEEASLSIADMADDETICGCN
CEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEECCC
GVDKGTIVNAITENGFTTVEEVTAKTKAGNSCGKCKPQIAQILQHTLGDDFVAAKPAGIC
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCC
GCTDLTRDQIVTQIRAKGLKTSKEVRHVLNFKNKGGCPKCRPAINYYLNMVYPHDHEDER
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHEEEECCCCCCHHH
ESRFANERYHANIQNDGTFSVIPQMRGGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGL
HHHHCCCCEEECCCCCCCEEECHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKEELPNIWEDLGMRSASAYGKKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYI
ECCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHEECEEEEEHHHHH
DTPHKFKMGVSGCPRSCVESGVKDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDE
CCCCCEECCCCCCCHHHHHCCCHHCEEEEECCCEEEEECCCCCCCCHHHHHEEECCCHHH
VIKLCGALMQYYRETGIYAERTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEA
HHHHHHHHHHHHHHCCCCHHHCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
EPWEAITSNAQARRIFEVEKV
CCHHHHHCCCHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]