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The map label for this gene is nreC

Identifier: 57650934

GI number: 57650934

Start: 2448232

End: 2448885

Strand: Reverse

Name: nreC

Synonym: SACOL2389

Alternate gene names: 57650934

Gene position: 2448885-2448232 (Counterclockwise)

Preceding gene: 57650935

Following gene: 57650932

Centisome position: 87.17

GC content: 33.94

Gene sequence:

>654_bases
TTGAAAATAGTCATTGCCGATGATCACGCTGTTGTCCGTACGGGGTTCTCTATGATTTTAAATTATCAAAATGATATGGA
AGTTGTTGCAACGGCTGCAGATGGCGTCGAAGCTTACCAAAAAGTAATGGAATATAAACCTGATGTGTTACTAATGGATT
TAAGTATGCCACCAGGTGAGTCAGGTCTTATCGCTACGAGTAAAATTGCTGACAGTTTTCCTGAAACTAAAATACTAATA
TTAACAATGTTTGATGATGAGGAGTATTTGTTCCATGTGTTGCGTAATGGTGCGAAAGGTTACATATTGAAAAATGCACC
TGATGAACAATTATTGTTAGCTATTCGAACTGTATATAAAGGTGAAACATATGTAGATATGAAACTTACAACATCTTTAG
TGAATGAATTTGTATCTAATTCAAATCAGGACACTGCAAACACAACAGATCCTTTTAAAATCTTATCAAAACGAGAACTA
GAAATATTGCCACTTATTGCCAAAGGTTACGGGAATAAAGAAATTGCAGAGAAATTATTTGTATCTGTGAAAACAGTAGA
AGCACATAAGACGCATATTATGACAAAGCTTGGCTTAAAGAGTAAACCTGAGCTAGTTGAATATGCATTGAAAAAGAAAT
TATTAGAGTTTTAG

Upstream 100 bases:

>100_bases
GAGTTAGTCAGCGGTAGCGTCAATATTGAAACGAAAATTGGTGAGGGTACCAATGTTACATTGAACATTCCAATTTGAAA
CATACATTGGGGGAATAAAA

Downstream 100 bases:

>100_bases
AGTTTGAAATTAATATAATTCAGTATAAAAGCGCGAGAGGAAATGATTATCTTCTGGCGCTTTTGTATGTTTCGAAATAT
ATTTTTTTATAAATCGAATG

Product: transcriptional regulatory protein DegU

Products: NA

Alternate protein names: Nitrogen regulation protein C

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILI
LTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF

Sequences:

>Translated_217_residues
MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILI
LTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF
>Mature_217_residues
MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILI
LTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. Phosphorylated nreC binds to a GC-rich palindromic sequence at the promoters of the nitrate (narGHJI) and nit

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788222, Length=215, Percent_Identity=35.3488372093023, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788521, Length=201, Percent_Identity=35.3233830845771, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786747, Length=211, Percent_Identity=29.8578199052133, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1790102, Length=205, Percent_Identity=31.219512195122, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1788712, Length=216, Percent_Identity=30.5555555555556, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787473, Length=187, Percent_Identity=33.1550802139037, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788546, Length=210, Percent_Identity=30, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1789937, Length=204, Percent_Identity=25.4901960784314, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NREC_STAA1 (A7X623)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442965.1
- ProteinModelPortal:   A7X623
- SMR:   A7X623
- STRING:   A7X623
- EnsemblBacteria:   EBSTAT00000002843
- GeneID:   5559412
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2375
- eggNOG:   COG2197
- GeneTree:   EBGT00050000024054
- HOGENOM:   HBG753323
- OMA:   FKEADWI
- ProtClustDB:   CLSK885949
- BioCyc:   SAUR418127:SAHV_2375-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 24369; Mature: 24369

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGE
CEEEEECCCHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCC
SGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYK
CCCEEEHHHHCCCCCCEEEEEEEECCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHC
GETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKRELEILPLIAKGYGNKEIAEKLF
CCEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHH
VSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF
HHHHEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGE
CEEEEECCCHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCC
SGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYK
CCCEEEHHHHCCCCCCEEEEEEEECCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHC
GETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKRELEILPLIAKGYGNKEIAEKLF
CCEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHH
VSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF
HHHHEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA