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The map label for this gene is ilvA2

Identifier: 57650733

GI number: 57650733

Start: 2110529

End: 2111797

Strand: Direct

Name: ilvA2

Synonym: SACOL2050

Alternate gene names: 57650733

Gene position: 2110529-2111797 (Clockwise)

Preceding gene: 57650732

Following gene: 57650752

Centisome position: 75.12

GC content: 32.07

Gene sequence:

>1269_bases
ATGACAGTCAAAACAACAGTTTCTACGAAAGATATCGATGAGGCATTTTTAAGACTTAAAGATATTGTCAAAGAAACACC
TTTACAATTAGACCATTACTTATCTCAAAAGTATGATTGTAAAGTCTATTTAAAACGAGAAGATTTACAATGGGTACGTT
CTTTTAAATTAAGAGGTGCTTACAACGCTATTTCTGTTTTATCAGATGAAGCTAAAAGTAAAGGTATTACATGTGCAAGT
GCAGGTAATCATGCTCAAGGTGTTGCCTATACAGCTAAAAAACTTAATTTAAACGCTGTTATCTTTATGCCAGTCACTAC
ACCTTTACAAAAGGTAAATCAAGTAAAGTTCTTTGGAAATAGTAACGTTGAAGTTGTACTCACTGGTGATACATTTGATC
ACTGTTTAGCTGAAGCTTTAACTTATACAAGTGAACATCAAATGAACTTTATAGATCCATTCAATAATGTTCATACAATT
TCTGGACAAGGTACGCTTGCTAAAGAAATGCTAGAACAAGCAAAGTCTGACAATGTTAACTTTGATTATCTATTTGCCGC
AATTGGTGGTGGCGGTTTAATTTCAGGTATTAGTACTTACTTTAAAACCTATTCACCTACCACGAAAATTATAGGTGTTG
AACCTTCAGGTGCAAGTAGTATGTATGAATCTGTTGTGGTAAATAATCAGGTAGTCACATTGCCTAATATCGATAAATTT
GTGGACGGTGCATCTGTAGCTAGAGTTGGCGATATTACATTTGAAATTGCAAAAGAAAATGTAGATGATTACGTTCAAGT
AGATGAAGGTGCAGTTTGTTCTACGATTTTAGATATGTATTCAAAACAAGCAATTGTAGCAGAACCTGCTGGCGCATTAA
GTGTAAGTGCGCTTGAAAACTATAAAGATCATATTAAAGGTAAAACAGTGGTTTGTGTCATTAGTGGTGGTAATAATGAT
ATTAATCGAATGAAAGAAATTGAAGAACGTTCATTACTATACGAAGAAATGAAGCATTACTTTATCTTAAATTTCCCTCA
ACGTCCAGGTGCATTGAGAGAATTTGTAAATGACGTATTAGGACCTCAAGACGATATTACTAAATTTGAATACTTAAAAA
AATCTTCTCAAAATACAGGTACTGTCATTATTGGTATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGT
GTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAA

Upstream 100 bases:

>100_bases
AGAATAAACTTGTAAATGGCTTAGATGACATTGCAATCACCCTACAATATGAATCATTAATAGAAAAATATGAAAAATCA
CTTTAAGGGAGTTGAATATT

Downstream 100 bases:

>100_bases
CACATAGTAAGAAAAACAGTCATAAATTGATTTCTAATTGAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTC
TCGTTTCGTCAGATTCAAAC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCAS
AGNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKF
VDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNND
INRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQR
VNHFDPSNIYINENKMLYSLLI

Sequences:

>Translated_422_residues
MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCAS
AGNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKF
VDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNND
INRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQR
VNHFDPSNIYINENKMLYSLLI
>Mature_421_residues
TVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASA
GNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTIS
GQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKFV
DGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDI
NRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQRV
NHFDPSNIYINENKMLYSLLI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family

Homologues:

Organism=Homo sapiens, GI11345492, Length=331, Percent_Identity=29.607250755287, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1790207, Length=365, Percent_Identity=35.0684931506849, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789505, Length=329, Percent_Identity=34.9544072948328, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789235, Length=271, Percent_Identity=23.6162361623616, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71991565, Length=350, Percent_Identity=31.7142857142857, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17537387, Length=411, Percent_Identity=28.9537712895377, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17508781, Length=322, Percent_Identity=27.0186335403727, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6320930, Length=358, Percent_Identity=38.8268156424581, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6322631, Length=313, Percent_Identity=34.5047923322684, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319788, Length=332, Percent_Identity=25.3012048192771, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355833, Length=350, Percent_Identity=31.4285714285714, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24645328, Length=254, Percent_Identity=29.5275590551181, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): THD1_STAA3 (Q2FF63)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_494665.1
- ProteinModelPortal:   Q2FF63
- SMR:   Q2FF63
- STRING:   Q2FF63
- EnsemblBacteria:   EBSTAT00000037621
- GeneID:   3915391
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_2014
- eggNOG:   COG1171
- GeneTree:   EBGT00050000024421
- HOGENOM:   HBG714501
- OMA:   DILMAHN
- ProtClustDB:   PRK08639
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820
- TIGRFAMs:   TIGR02079

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.3.1.19

Molecular weight: Translated: 46938; Mature: 46807

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGA
CEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCC
YNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGN
HHHHHHHHHHHHHCCEEEECCCCCCCCEEEEHEEECCEEEEEEECCCHHHHCCEEEEECC
SNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTISGQGTLAKEMLEQAKSDNVN
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCC
FDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKF
EEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCHHHH
VDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALEN
HCCCCCEEECCEEEEEECCCCCCCEECCCCHHHHHHHHHHCCCEEEECCCCCEEHHHHHH
YKDHIKGKTVVCVISGGNNDINRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVL
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHC
GPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQRVNHFDPSNIYINENKMLYSL
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCEEEEE
LI
CC
>Mature Secondary Structure 
TVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGA
EEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCC
YNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGN
HHHHHHHHHHHHHCCEEEECCCCCCCCEEEEHEEECCEEEEEEECCCHHHHCCEEEEECC
SNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTISGQGTLAKEMLEQAKSDNVN
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCC
FDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKF
EEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCHHHH
VDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALEN
HCCCCCEEECCEEEEEECCCCCCCEECCCCHHHHHHHHHHCCCEEEECCCCCEEHHHHHH
YKDHIKGKTVVCVISGGNNDINRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVL
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHC
GPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQRVNHFDPSNIYINENKMLYSL
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCEEEEE
LI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA