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The map label for this gene is leuC

Identifier: 57650731

GI number: 57650731

Start: 2108571

End: 2109941

Strand: Direct

Name: leuC

Synonym: SACOL2048

Alternate gene names: 57650731

Gene position: 2108571-2109941 (Clockwise)

Preceding gene: 57650730

Following gene: 57650732

Centisome position: 75.05

GC content: 37.93

Gene sequence:

>1371_bases
ATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGCGAACCGCAACTATTATACAT
TGATTTACACCTTATACATGAAGTTACTTCTCCTCAAGCATTTGAAGGACTTAGGCTTCAAAACAGAAAATTAAGACGCC
CAGATTTAACATTTGCAACACTCGATCACAATGTTCCTACTATTGATATATTCAATATTAAAGATGAAATTGCAAACAAA
CAAATCACAACATTACAAAAAAACGCCATAGATTTTGGGGTGCATATTTTTGATATGGGTTCTGATGAACAAGGTATTGT
TCACATGGTAGGACCTGAGACAGGACTTACACAGCCTGGCAAGACAATCGTTTGTGGTGACTCTCACACAGCAACACATG
GAGCATTTGGTGCTATTGCATTTGGAATTGGAACAAGTGAAGTTGAACATGTTTTCGCAACTCAAACGCTATGGCAAACA
AAACCCAAAAACTTAAAAATCGATATTAATGGTACCTTACCAACAGGCGTCTATGCTAAGGACATTATTCTGCATTTAAT
TAAAACGTATGGTGTTGACTTTGGTACAGGCTATGCTTTGGAATTTACTGGCGAAACAATTAAAAACCTTTCAATGGATG
GTCGAATGACTATTTGTAACATGGCTATCGAAGGTGGTGCCAAATACGGCATAATCCAACCTGATGATATAACATTTGAA
TATGTTAAAGGGAGACCATTTGCCGATAACTTCGCTAAATCAGTTGATAAGTGGCGTGAGCTATATTCTGATGACGACGC
GATATTTGATCGTGTAATTGAACTTGATGTTTCAACATTAGAACCACAAGTGACATGGGGAACTAATCCTGAAATGGGTG
TTAATTTCAGTGAACCATTCCCTGAAATCAATGATATCAACGATCAACGTGCGTATGATTATATGGGGTTAGAACCAGGT
CAAAAAGCTGAAGACATCGACTTAGGGTATGTTTTTCTCGGTTCATGTACAAATGCTAGACTATCAGATTTGATTGAAGC
TAGTCATATTGTTAAAGGAAATAAAGTTCATCCAAATATTACAGCTATTGTCGTACCAGGTTCTCGTACAGTAAAAAAAG
AAGCAGAAAAATTAGGTCTAGATACTATCTTTAAAAATGCAGGATTTGAATGGCGTGAACCAGGATGTTCAATGTGTTTA
GGCATGAATCCTGACCAAGTACCTGAGGGCGTACATTGTGCATCTACAAGTAATCGAAACTTTGAAGGACGACAAGGCAA
AGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCAGCAGCAGCTATTCATGGTAAATTTGTGGACGTAAGAAAGG
TGGTTGTTTAA

Upstream 100 bases:

>100_bases
GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAAATTCTATCTCAAAAATTGAA
TCACTAAGGGGGAGATGTAA

Downstream 100 bases:

>100_bases
ATGGCAGCAATCAAACCTATTACAACATATAAAGGTAAAATAGTCCCTCTCTTCAACGACAATATCGATACAGACCAAAT
CATTCCTAAGGTACACTTAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANK
QITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFE
YVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPG
QKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCL
GMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

Sequences:

>Translated_456_residues
MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANK
QITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFE
YVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPG
QKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCL
GMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV
>Mature_455_residues
GQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQ
ITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTK
PKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFEY
VKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQ
KAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLG
MNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=371, Percent_Identity=27.2237196765499, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI8659555, Length=447, Percent_Identity=25.0559284116331, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=461, Percent_Identity=23.644251626898, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=54.6236559139785, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=26.6483516483516, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081781, Length=340, Percent_Identity=23.2352941176471, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=24.9258160237389, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=371, Percent_Identity=29.6495956873315, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=371, Percent_Identity=29.6495956873315, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=303, Percent_Identity=28.7128712871287, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=456, Percent_Identity=25.6578947368421, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=53.1914893617021, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320440, Length=416, Percent_Identity=28.8461538461538, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=376, Percent_Identity=29.5212765957447, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=383, Percent_Identity=30.0261096605744, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281365315, Length=392, Percent_Identity=27.5510204081633, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17864292, Length=392, Percent_Identity=27.5510204081633, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161076999, Length=392, Percent_Identity=27.5510204081633, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571643, Length=357, Percent_Identity=26.890756302521, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24645686, Length=457, Percent_Identity=25.8205689277899, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137564, Length=456, Percent_Identity=25.219298245614, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_STAA1 (A7X4N3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442634.1
- ProteinModelPortal:   A7X4N3
- SMR:   A7X4N3
- STRING:   A7X4N3
- EnsemblBacteria:   EBSTAT00000003931
- GeneID:   5560206
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2044
- eggNOG:   COG0065
- GeneTree:   EBGT00050000024545
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   SAUR418127:SAHV_2044-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50327; Mature: 50196

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFAT
CCCHHHHHHHCCEEEEECCCCCEEEEEEEEEEHHCCCCCHHCCCHHCCCCCCCCCCEEEE
LDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPG
ECCCCCEEEEECCHHHHCCCHHHHHHHCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAK
CEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
DIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFE
HHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCCCCEEEEEEEECCCCEECCCCCCCCEEE
YVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPF
EECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
PEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNI
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCE
TAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRN
EEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCC
FEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV
CCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHEECC
>Mature Secondary Structure 
GQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFAT
CCHHHHHHHCCEEEEECCCCCEEEEEEEEEEHHCCCCCHHCCCHHCCCCCCCCCCEEEE
LDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPG
ECCCCCEEEEECCHHHHCCCHHHHHHHCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAK
CEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
DIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFE
HHHHHHHHHHCCCCCCCEEEEECCHHHHHCCCCCCEEEEEEEECCCCEECCCCCCCCEEE
YVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPF
EECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
PEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNI
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCE
TAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRN
EEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCC
FEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV
CCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA