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The map label for this gene is ilvB [H]

Identifier: 57650726

GI number: 57650726

Start: 2102785

End: 2104554

Strand: Direct

Name: ilvB [H]

Synonym: SACOL2043

Alternate gene names: 57650726

Gene position: 2102785-2104554 (Clockwise)

Preceding gene: 57650725

Following gene: 57650727

Centisome position: 74.85

GC content: 36.61

Gene sequence:

>1770_bases
ATGTCTAAAACTCAACATGAAGTAAACCAAAATATTGACCCTTTAAAAATGGCTGAATCACTTGAACCTGAACAACTAAA
TGAAAAAACTTTAAATGATATGCGTTCAGGATCAGAAGTGCTAGTAGAAGCTCTACTTAAAGAAAATGTGGATTATTTAT
TCGGTTATCCTGGTGGTGCCGTACTACCTTTATATGACACGTTTTATGATGGTAAAATCAAACATATTTTAGCAAGACAC
GAACAAGGTGCTGTTCATGCTGCAGAAGGTTATGCACGTGTATCTGGTAAAACTGGCGTCGTTGTAGTTACAAGCGGTCC
AGGTGCAACTAATGTAATGACAGGTATTACGGATGCACATTGCGACTCTTTACCTCTAGTTGTATTCACTGGACAAGTTG
CTACACCAGGCATTGGTAAAGATGCATTCCAAGAAGCGGATATTCTATCTATGACTTCACCAATTACAAAACAAAATTAT
CAAGTGAAACGTGTTGAAGATATCCCTAAAATCGTACACGAAGCTTTCCATGTAGCTAATTCTGGACGCAAAGGTCCTGT
AGTGATTGATTTTCCAAAAGATATGGGTGTTTTAGCTACAAATGTGGATTTATGCGACGAAATCAATATTCCAGGTTATG
AAGTTGTTACAGAACCAGAAAATAAAGACATTGACACTTTCATCTCACTTTTAAAAGAAGCGAAAAAGCCTGTCGTATTA
GCCGGCGCAGGTATTAATCAATCAAAATCAAATCAATTATTAACACAGTTTGTTAATAAACATCAAATCCCAACAGTTAC
TACTTTACTAGGTCTAGGTGCTGTACCTTATGAGGATACACTATTTTTAGGTATGGGAGGAATGCATGGTTCTTATGCTA
GTAACATGGCATTAACTGAGTGTGATTTACTCATTAATTTAGGTAGCCGCTTCGATGATAGATTAGCAAGCAAACCTGAT
GCCTTTGCACCTAACGCCAAAATTGTACATGTAGATATTGATCCTTCAGAAATCAATAAAGTTATTCATGTAGATTTAGG
TATTATTGCAGACTGTAAAAGATTTTTAGAATGTTTAAATGATAAAAATGTTGAGACTATAGAACACAGTGACTGGGTTA
AACATTGTCAAAATAATAAGCAGAAACACCCATTTAAACTTGGTGAAGAAGATCAAGTATTTTGTAAGCCACAACAAACA
ATCGAATATATCGGCAAAATTACAAATGGTGAAGCAATTGTTACTACAGACGTGGGACAACATCAAATGTGGGCAGCTCA
ATTTTATCCATTTAAAAATCACGGACAATGGGTTACAAGCGGTGGTTTAGGAACAATGGGATTCGGTATTCCTTCGTCAA
TTGGTGCCAAATTAGCTAATCCTGATAAAACAGTCGTATGTTTCGTCGGTGACGGTGGTTTCCAAATGACAAACCAAGAA
ATGGCACTTTTACCCGAATATGGTTTAGATGTCAAAATCGTACTAATCAATAATGGAACATTAGGTATGGTTAAACAATG
GCAAGATAAGTTCTTTAATCAACGCTTCTCACACTCAGTATTTAATGGTCAACCTGATTTTATGAAAATGGCAGAAGCAT
ATGGCGTCAAAGGTTTCTTAATCGATAAGCCAGAACAACTGGAAGAACAATTAGATGCAGCGTTTGCTTATCAAGGACCA
GCTTTAATTGAGGTTCGTATTTCCCCTACTGAAGCTGTAACCCCAATGGTTCCGAGTGGCAAATCAAATCATGAAATGGA
GGGCTTATAA

Upstream 100 bases:

>100_bases
AGCTCGTTATACTGCCCTAGTAACTAGCGCAAATACAGGTGGCGTCATGCAAGTCCCTGAGAATTTAATTTAATTTATTT
TTATATTGGAGATGGTTAAA

Downstream 100 bases:

>100_bases
TGACAAGAATTCTTAAATTACAAGTTGCGGATCAAGTCAGCACGCTAAATCGAATTACAAGTGCTTTTGTTCGCCTACAA
TATAATATCGATACATTACA

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARH
EQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVL
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPD
AFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQT
IEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGP
ALIEVRISPTEAVTPMVPSGKSNHEMEGL

Sequences:

>Translated_589_residues
MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARH
EQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVL
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPD
AFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQT
IEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGP
ALIEVRISPTEAVTPMVPSGKSNHEMEGL
>Mature_588_residues
SKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHE
QGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQ
VKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVLA
GAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDA
FAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQTI
EYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQEM
ALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPA
LIEVRISPTEAVTPMVPSGKSNHEMEGL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=24.1935483870968, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=24.0574506283662, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=42.5795053003534, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=41.7253521126761, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=32.780847145488, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=26.4705882352941, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=23.8181818181818, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=25.1346499102334, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=25.1346499102334, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=604, Percent_Identity=25.6622516556291, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=604, Percent_Identity=41.5562913907285, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=24.0246406570842, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=487, Percent_Identity=22.17659137577, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=488, Percent_Identity=22.9508196721311, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=484, Percent_Identity=21.900826446281, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320588, Length=613, Percent_Identity=19.4127243066884, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19922626, Length=566, Percent_Identity=27.208480565371, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64492; Mature: 64361

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGA
CCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCC
VLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAH
EEEEHHHHCCCHHHHHHHHCCCCCEECCCCEEEECCCCCEEEEECCCCCCHHHCCCCCCC
CDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQVKRVEDIPKIVHEAFHVAN
CCCCCEEEEECCCCCCCCCHHHHHCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
SGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVL
CCCCCCEEEECCCCCCEEEECCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTE
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCCHHH
CDLLINLGSRFDDRLASKPDAFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLN
HHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCHHHCCEEEEECCHHHHHHHHHHHHHC
DKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQTIEYIGKITNGEAIVTTDVGQ
CCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCEEEEECCCC
HQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
CCEEHHHCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCEECCCC
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFL
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCEEE
IDKPEQLEEQLDAAFAYQGPALIEVRISPTEAVTPMVPSGKSNHEMEGL
ECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGA
CCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCC
VLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAH
EEEEHHHHCCCHHHHHHHHCCCCCEECCCCEEEECCCCCEEEEECCCCCCHHHCCCCCCC
CDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQVKRVEDIPKIVHEAFHVAN
CCCCCEEEEECCCCCCCCCHHHHHCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
SGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVL
CCCCCCEEEECCCCCCEEEECCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTE
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCCHHH
CDLLINLGSRFDDRLASKPDAFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLN
HHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCHHHCCEEEEECCHHHHHHHHHHHHHC
DKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQTIEYIGKITNGEAIVTTDVGQ
CCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCEEEEECCCC
HQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQE
CCEEHHHCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCEECCCC
MALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFL
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCEEE
IDKPEQLEEQLDAAFAYQGPALIEVRISPTEAVTPMVPSGKSNHEMEGL
ECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]