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The map label for this gene is hlb

Identifier: 57650692

GI number: 57650692

Start: 2063726

End: 2064718

Strand: Direct

Name: hlb

Synonym: SACOL2003

Alternate gene names: 57650692

Gene position: 2063726-2064718 (Clockwise)

Preceding gene: 57650691

Following gene: 57650697

Centisome position: 73.46

GC content: 33.64

Gene sequence:

>993_bases
ATGGTGAAAAAAACAAAATCCAATTCACTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACT
TACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTAT
CGACCGTTTTGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGAT
GTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAATATCCTTATCA
AACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGCAGAAGATGGTG
GCGTAGCGATTGTAAGTAAATATCCTATTAAAGAAAAAATCCAGCATGTTTTCAAAAGCGGTTGTGGATTCGATAATGAT
AGCAACAAAGGCTTTGTTTATACAAAAATAGAGAAAAATGGTAAGAACGTTCACGTTATCGGTACACATACACAATCTGA
AGATTCACGTTGTGGTGCTGGACATGATCGAAAAATTAGAGCTGAACAAATGAAAGAAATCAGTGACTTTGTTAAAAAGA
AAAATATCCCTAAAGATGAAACGGTATATATAGGTGGCGACCTTAATGTCAATAAAGGCACTCCAGAGTTCAAAGATATG
CTTAAAAACTTGAATGTAAATGATGTTCTATATGCAGGTCATAATAGCACATGGGACCCTCAATCAAATTCAATTGCGAA
ATATAATTACCCTAATGGTAAACCAGAACATTTAGACTATATATTTACAGATAAAGATCATAAACAACCAAAACAATTAG
TCAATGAAGTTGTGACTGAAAAACCTAAGCCATGGGATGTATATGCGTTCCCATATTACTACGTTTACAATGATTTTTCA
GATCATTACCCAATCAAAGCCTATAGTAAATAG

Upstream 100 bases:

>100_bases
ATTTACTTAAAAATATAAATTCTATTTAATAATTAATTTAAATTTAGTTAATCAATTTTGCATCTATTTTGTTGTAAGCT
ATATAAAAGGAGTGATAATG

Downstream 100 bases:

>100_bases
TGCTCAACTAACTAATAACTTGCTTCGTTCTAAAAGGACGAAGCGAGTTATATTGTTAAAATTTGAATTGACTTACATTT
TAATAAAATCATCTTAACAA

Product: phospholipase C

Products: NA

Alternate protein names: Beta-hemolysin; Beta-toxin; Sphingomyelinase; SMase

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLKLVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNND
VVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDND
SNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDM
LKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFS
DHYPIKAYSK

Sequences:

>Translated_330_residues
MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLKLVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNND
VVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDND
SNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDM
LKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFS
DHYPIKAYSK
>Mature_330_residues
MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLKLVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNND
VVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDND
SNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDM
LKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFS
DHYPIKAYSK

Specific function: Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Beta-hemolysin is a phospholipase C with specific activity toward sphingomyelins. Has a high specificity for sphingomyelin, hydrolyzes lysophosphatidylcholine at a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral sphingomyelinase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHLC_STAA8 (Q2FWP1)

Other databases:

- EMBL:   CP000253
- EMBL:   CP000253
- RefSeq:   YP_500651.1
- RefSeq:   YP_500724.1
- ProteinModelPortal:   Q2FWP1
- SMR:   Q2FWP1
- STRING:   Q2FWP1
- EnsemblBacteria:   EBSTAT00000029298
- GeneID:   3919662
- GeneID:   3921858
- eggNOG:   NOG17887
- GeneTree:   EBGT00050000025168
- BioCyc:   SAUR93061:SAOUHSC_02163-MONOMER
- BioCyc:   SAUR93061:SAOUHSC_02240-MONOMER
- GO:   GO:0019835
- GO:   GO:0009405
- InterPro:   IPR005135
- InterPro:   IPR017766
- TIGRFAMs:   TIGR03395

Pfam domain/function: PF03372 Exo_endo_phos; SSF56219 Exo_endo_phos

EC number: =3.1.4.3

Molecular weight: Translated: 37238; Mature: 37238

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLKLVSHNVYMLSTVLYPN
CCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCEEEEEEEECCC
WGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDK
CCCCHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNVHVI
CCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEE
GTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDM
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCCCHHHHHH
LKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTE
HHCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHC
KPKPWDVYAFPYYYVYNDFSDHYPIKAYSK
CCCCCEEEECEEEEEECCCCCCCCEEECCC
>Mature Secondary Structure
MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLKLVSHNVYMLSTVLYPN
CCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCEEEEEEEECCC
WGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDK
CCCCHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNVHVI
CCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEE
GTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDM
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCCCHHHHHH
LKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTE
HHCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHC
KPKPWDVYAFPYYYVYNDFSDHYPIKAYSK
CCCCCEEEECEEEEEECCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8914839