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The map label for this gene is rluB [H]

Identifier: 57650449

GI number: 57650449

Start: 1574943

End: 1575680

Strand: Reverse

Name: rluB [H]

Synonym: SACOL1536

Alternate gene names: 57650449

Gene position: 1575680-1574943 (Counterclockwise)

Preceding gene: 57650450

Following gene: 57650448

Centisome position: 56.09

GC content: 33.88

Gene sequence:

>738_bases
ATGACTAAAGAATTAGAACGATTACAAAAACGTATAGCTAATAGCGGTTATACTTCAAGACGTAAGGCAGAAACTTTAAT
TTCTGAAGGAAAAGTGAAAGTGAATGGTACGACTGTCACTGAATTAGGAACAAAAGTGAAGCCATCAGATACGATTGAAG
TTGAAGGTATTAAAATAGAACTAGAAGACAAAATTTACATTTTATTCCATAAACCAACTCAAGTGATAACAAGTGTATCT
GATGATAGAGGACGTACAGTGGTTACAGATTACTTTAAAGATATAGAAGCACGTATTTATCCTGTTGGTCGTTTAGACTA
TGATACTTCTGGATTGTTATTACTCACAAATGATGGTGAATTTACTAATTTAATGACACATCCAAGATATCAAATTAAGA
AAAAATATGTTGCGAAATTAAAAGGTTATTTAATGAGAGAAGAAGTGAAAGCGCTAGAAAAAGGTATTGAATTAGAAGAT
GGTATGACGCAACCGGCTGAAGTGAAAGTTAAGAAACAAGATAAAGATAAAAACACAACATTGGTTGAAATTACAATTAC
AGAAGGTCGTAATCGCCAAGTCAGAAGAATGTTCGAACATTTTGGTCATCAAGTGACGAAACTGTCTCGTATTGAATATG
GGCCTTTAAATGTTGTCGGTTTGAATGCTGGTGAAGGACGTGTATTGACGCCTCATGAAGTAAAAGTGATGCGTCATTTA
GCAGAACATGGGAAATAA

Upstream 100 bases:

>100_bases
TTTGGTATTTCCAATATAGAAGATTTGCCGACAACTGAAGAAGACGATGAAGAAATGGATGCTTTTTTCAGTAATCTAGT
CAATCAAAAAGGAGAAAATA

Downstream 100 bases:

>100_bases
TTAAATAAAATATGTATTTATCACAAAGTTTGAGAATTTTTTCACAAAATTTTAGGTTTTTAATAACTGTATGCGCTTTC
CTGTGATATAATACTTTTGT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVS
DDRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHL
AEHGK

Sequences:

>Translated_245_residues
MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVS
DDRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHL
AEHGK
>Mature_244_residues
TKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSD
DRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELEDG
MTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHLA
EHGK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=34.412955465587, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=32.4786324786325, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=34.4632768361582, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27970; Mature: 27838

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCEEHHHCCCCCCCCCCEEEEEEEEE
LEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGE
EECEEEEEEECCHHHHHCCCCCCCCEEHHHHHHCCHHEEEECCCCCCCCCCEEEEECCCC
FTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELEDGMTQPAEVKVKKQDKDKNTT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCE
LVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHL
EEEEEEECCCCHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCCCEECHHHHHHHHHH
AEHGK
HHHCC
>Mature Secondary Structure 
TKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGTKVKPSDTIEVEGIKIE
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCEEHHHCCCCCCCCCCEEEEEEEEE
LEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPVGRLDYDTSGLLLLTNDGE
EECEEEEEEECCHHHHHCCCCCCCCEEHHHHHHCCHHEEEECCCCCCCCCCEEEEECCCC
FTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELEDGMTQPAEVKVKKQDKDKNTT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCE
LVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNVVGLNAGEGRVLTPHEVKVMRHL
EEEEEEECCCCHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCCCEECHHHHHHHHHH
AEHGK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]