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The map label for this gene is ansA [H]

Identifier: 57650433

GI number: 57650433

Start: 1557882

End: 1558850

Strand: Direct

Name: ansA [H]

Synonym: SACOL1519

Alternate gene names: 57650433

Gene position: 1557882-1558850 (Clockwise)

Preceding gene: 57650405

Following gene: 57650439

Centisome position: 55.45

GC content: 34.88

Gene sequence:

>969_bases
ATGAAACATCTACTTGTTATTCATACTGGTGGCACCATTAGTATGTCACAAGACCAATCTAATAAAGTAGTAACAAATGA
TATCAACCCTATTTCAATGCATCAAGATGTCATAAATCAATATGCACAAATAGATGAATTAAATCCTTTTAATGTACCAT
CACCTCATATGACAATCCAACATGTTAAACAATTAAAGGATATTATTTTAGAAGCAGTAACAAATAAATATTATGATGGT
TTCGTTATCACGCATGGTACCGATACGTTAGAAGAAACTGCCTTTTTACTTGATTTAATATTAGGTATCGAGCAACCTGT
TGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGATTATATAATTATATTTCCGCTATTCGCGTTG
CCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGCGCGTAATGTTACCAAA
ACACATACGTCTAATACAAACACATTTCAAAGTCCAAATCATGGTCCGCTAGGTGTATTGACAAAGGATCGTGTGCAATT
CCATCATATGCCATATCGCCAACAAGCATTGGAAAATGTCAATGACAAACTAAATGTACCATTAGTAAAAGCATATATGG
GTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAAC
ATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCTAGTTTCACGTTCCTTTAA
TGGTATTGTGAGTCCAACTTACGCATACGATGGTGGTGGTTACCAACTCGCACAACAAGGTTTTATTTTTTCTAACGGCT
TGAATGGTCCAAAAGCAAGATTAAAATTATTAGTCGCGTTAAGCAACAATTTAGATAAAGCTGAAATCAAATCATATTTT
GAATTATAA

Upstream 100 bases:

>100_bases
TAATGCAATATTAATGGCTTTCATGCGTCATTTCCCTTCCTTGTCGTTTTATGAATAATATTTTATCATAATTAGGTAAA
TTAAATATAGGAGGAAATAT

Downstream 100 bases:

>100_bases
TGAATACGTAACATAAAAAGGTCTATGTACAATCATTAGTCATCAACCTCATTTGACATATCATGAATGTCAGTCGTTGT
AAAACTAAGTTATTTAGTAC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDG
FVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYF
EL

Sequences:

>Translated_322_residues
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDG
FVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYF
EL
>Mature_322_residues
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDG
FVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYF
EL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=304, Percent_Identity=23.6842105263158, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789327, Length=248, Percent_Identity=35.8870967741936, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788065, Length=341, Percent_Identity=28.7390029325513, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193207372, Length=346, Percent_Identity=24.5664739884393, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=346, Percent_Identity=24.5664739884393, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558278, Length=346, Percent_Identity=24.5664739884393, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=346, Percent_Identity=24.5664739884393, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=338, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323187, Length=338, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323186, Length=338, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323184, Length=338, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320527, Length=252, Percent_Identity=30.5555555555556, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645530, Length=231, Percent_Identity=29.8701298701299, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24639609, Length=240, Percent_Identity=27.0833333333333, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35579; Mature: 35579

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQ
CCEEEEEECCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
HVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIG
HHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
SDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVL
CCHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCE
TKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
ECCCHHHHCCCHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCC
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKAR
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHCCEEEECCCCCHHHH
LKLLVALSNNLDKAEIKSYFEL
HEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQ
CCEEEEEECCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
HVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIG
HHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
SDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVL
CCHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCE
TKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
ECCCHHHHCCCHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCC
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKAR
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHCCEEEECCCCCHHHH
LKLLVALSNNLDKAEIKSYFEL
HEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]