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The map label for this gene is ypcP [H]

Identifier: 57650395

GI number: 57650395

Start: 1517390

End: 1518268

Strand: Reverse

Name: ypcP [H]

Synonym: SACOL1479

Alternate gene names: 57650395

Gene position: 1518268-1517390 (Counterclockwise)

Preceding gene: 57650396

Following gene: 57650394

Centisome position: 54.04

GC content: 32.54

Gene sequence:

>879_bases
ATGCCTAATAAAATATTACTTGTAGATGGTATGGCGCTATTATTTAGACATTTCTATGCTACAAGTCTTCATAAACAATT
TATGTACAATTCACAAGGTGAACCTACAAATGGAATACAAGGATTTGTGCGTCATATCTTTTCGGCAATACATGAAATAC
GCCCTACACATGTAGCTGTATGTTGGGATATGGGACAATCAACTTTTAGAAATGATATGTTTGATGGTTATAAGCAAAAT
CGTTCTGCACCACCAGAAGAATTGATACCACAATTTGATTATGTTAAAGAAATTTCAGAGCAATTTGGCTTTGTAAATAT
TGGCGTTAAAAACTATGAAGCGGATGATGTTATAGGTACATTAGCACAACAATATTCAACTGATAACGATGTCTATATTA
TTACGGGCGACAAAGATTTACTGCAATGTATTAATGACAATGTTGAAGTTTGGCTAATTAAAAAAGGTTTTAACATTTAT
AATAGATATACATTACATCGTTTTAACGAAGAATATGCCCTTGAACCACAACAACTAATCGATATTAAAGCGTTTATGGG
TGATACAGCAGATGGATATGCGGGTGTTAAAGGTATTGGCGAGAAAACGGCTATTAAGTTAATTCAGCAATATCAAAGCG
TTGAAAATGTGGTTGAAAACATTGATGCATTATCAGCTGGGCAACGTAATAAGATTAATGACAATTTAGATGAACTATAT
TTATCGAAGCGACTGGCTGAAATTCACACACAAGTACCAATTGATAGTGAAGCGCTTTTTGAGAAAATGTCATTTGCAAC
AACGCTTAATCATATTTTATCAATTTGTAACGAACATGAATTACATGTTTCAGGAAAATATATTTCATCCCATTTTTAA

Upstream 100 bases:

>100_bases
TTATTAGCATTTGATTTAGATACAACACTTATCAATCAATTGAAAGATAAACATCAACAACTGAAAACTATTTTATATTA
AGAAAGAAGGAACGTTTTAA

Downstream 100 bases:

>100_bases
AAAGTATAAGATTTTATGAAAAATTAAACCAATTAAGTATAAAAAGACTATTTCTATCGATACAAGGTAGAAATAGTCTT
TTTGCGTATTTGGATATGTC

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQN
RSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELY
LSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF

Sequences:

>Translated_292_residues
MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQN
RSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELY
LSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF
>Mature_291_residues
PNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNR
SAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYN
RYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELYL
SKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=263, Percent_Identity=34.2205323193916, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI226510970, Length=259, Percent_Identity=28.1853281853282, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 33402; Mature: 33271

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAV
CCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
CWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGT
EECCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHH
LAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYNRYTLHRFNEEYALEPQQLI
HHHHHCCCCCEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCHHHHH
DIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELY
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCC
>Mature Secondary Structure 
PNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAV
CCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
CWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGT
EECCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHH
LAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYNRYTLHRFNEEYALEPQQLI
HHHHHCCCCCEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCHHHHH
DIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELY
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]