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The map label for this gene is lplA [H]

Identifier: 57650219

GI number: 57650219

Start: 1043618

End: 1044604

Strand: Reverse

Name: lplA [H]

Synonym: SACOL1034

Alternate gene names: 57650219

Gene position: 1044604-1043618 (Counterclockwise)

Preceding gene: 57650226

Following gene: 57650217

Centisome position: 37.18

GC content: 30.5

Gene sequence:

>987_bases
ATGAAATTCATTAGTAATAATAATATTACAGATCCAACTTTAAACTTAGCAATGGAAGAATATGTTTTAAAAAATTTACC
AGCAGAAGAAAGTTACTTTTTATTTTACATAAATAGACCATCTATCATTGTTGGAAAGAATCAAAATACGATAGAGGAAG
TAAATCAGACATATATCGATGCTCACAACATCGATGTAGTAAGAAGAATTTCTGGTGGTGGCGCTGTTTATCATGATACT
GGCAATTTAAACTTTAGTTTTATAACAGATGATGATGGTAACAGTTTCCACAATTTCCAAAAATTCACTGAACCAATTGT
TCAAGCATTACAATCTTTAGGCGTAAATGCTGAATTAACTGGTCGTAACGATATTCAAGTTGGACAAGCGAAAATCTCAG
GGAATGCTATGGTTAAAGTTAAAAATAGAATGTTTAGTCATGGTACGTTGATGTTAAATAGTGATTTAGACGAAGTTCAG
AATGCACTAAAAGTTAATCCAGCTAAGATTAAATCAAAAGGTATTAAATCTGTTCGTAAACGGGTAGCAAACATTCAAGA
ATTTTTAAATGACCCTTTAGAAATTGAAGAATTTAAAAAAATCATCTTGAAAACTATCTTTGGAGAAACTGAAGTAGAAG
AATATAAATTAACGGATGAAGATTGGGAAAATATTGAAAAGTTAAGTAACGACAAATATAGAACTTGGGAATGGAATTAT
GGTAGAAACCCTAAATATAACTTTGAACGCGAGGAAAAATTTGAAAAAGGATTTGTACAAATTAAGTTTGATGTTAAACG
AGGTAAAATCGAACATGCAAAAATATTCGGTGATTTCTTTGGTGTCGGAGATGTCACTGATCTTGAAAATGCATTAGTTG
GCTGTCTACACGACTTTGAGCACATAGAAGAAGCACTATCAGAATATGATTTATATCATTATTTCGGTGACATAGATAGA
CATGAATTAATTAGATTAATGTCATAA

Upstream 100 bases:

>100_bases
CCGATATTTAGAATGATTTGTGAGTATATAATTGGCATTTTGAGCAAAGTTAAGGCATAAATAAGTAGAGAATATATATA
GATATAAGGGAGAGATTGTA

Downstream 100 bases:

>100_bases
ACGATTAAAGGACTATTCTTACATGTATGAGATGTTAAGAATAGTCCTTTTATGATTGCGTTATGGAATTCGAATCAATG
TTATTCACCGAGAAATGCCT

Product: lipoate-protein ligase A family protein

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDT
GNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWEWNY
GRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDR
HELIRLMS

Sequences:

>Translated_328_residues
MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDT
GNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWEWNY
GRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDR
HELIRLMS
>Mature_328_residues
MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDT
GNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWEWNY
GRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDR
HELIRLMS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=274, Percent_Identity=30.2919708029197, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI21729884, Length=274, Percent_Identity=30.2919708029197, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI21729882, Length=274, Percent_Identity=30.2919708029197, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI21729880, Length=274, Percent_Identity=30.2919708029197, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI21729878, Length=274, Percent_Identity=30.2919708029197, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1790846, Length=302, Percent_Identity=34.1059602649007, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71981909, Length=251, Percent_Identity=33.0677290836653, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322415, Length=290, Percent_Identity=30.3448275862069, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28573464, Length=267, Percent_Identity=28.4644194756554, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37928; Mature: 37928

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYID
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHCC
AHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELT
CCCCEEEEEECCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKVNPAKIKSKGIKSVRK
CCCCCEECEEEECCCEEEEEECHHHCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
RVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWEWNY
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHCCCCEEEEECCC
GRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFE
CCCCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
HIEEALSEYDLYHYFGDIDRHELIRLMS
HHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYID
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHCC
AHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELT
CCCCEEEEEECCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKVNPAKIKSKGIKSVRK
CCCCCEECEEEECCCEEEEEECHHHCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
RVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWEWNY
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHCCCCEEEEECCC
GRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFE
CCCCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
HIEEALSEYDLYHYFGDIDRHELIRLMS
HHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]