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The map label for this gene is radA [H]

Identifier: 57238077

GI number: 57238077

Start: 1254887

End: 1256227

Strand: Reverse

Name: radA [H]

Synonym: CJE1339

Alternate gene names: 57238077

Gene position: 1256227-1254887 (Counterclockwise)

Preceding gene: 57238078

Following gene: 57238076

Centisome position: 70.66

GC content: 35.65

Gene sequence:

>1341_bases
ATGGCAAAAAATAAAGCACTTTTTGAATGTCAAGCCTGTGGAAATCAACAAAGTAAATGGCTTGGAAAATGTCCTGATTG
TGGAGCTTGGGATAGTTTTGTAGAATTAAAAGCTGAGCGGATTAAAGTTTTAAAAGAGCTTGCACAAGTTAGTATGAAAA
CAAGTGAAGCTGTTTGTATTGAAGATGTGGAGTTAGAACATTTTACAAGATACAGCACAGATGATAATGAGCTTGATTTG
GTTTTAGGTGGAGGGCTTGTTGAAGGTTCTTTAGTGCTTATAGGCGGAAGTCCAGGTGTGGGAAAATCTACGCTTTTGTT
AAAAATTGCTTCAAATTTAGCTAAACAGGGTAAAAAAGTGCTTTATGTAAGTGGCGAAGAAAGTAAAGCCCAGATTAAAT
TAAGAGCTGATCGTCTTGAGGCTAATACTCCGAATTTATTTTTACTTACTGAACTTTGCCTTGAAAATATTTTAGAAGAA
TTGCACAAAAAAGATTATAGCATTCTTATCGTTGATTCTATACAAACTCTATATTCAAATAAAATCACTTCAGCAGCAGG
AAGCATCACTCAGGTGCGTGAGATTACTTTTGAACTTATGCGTGTTAGCAAGGCTTATAATATCAGTACTTTTATCATAG
GGCATATTACTAAAGAAGGTGCTATAGCAGGGCCTAGGGTTCTTGAGCATATGGTAGATGTGGTGCTTTATTTTGAAGGA
GATGCTACTAAAGAAATCAGACTTTTAAGAGGCTTTAAAAATCGTTTTGGTGGAACGAATGAAGTAGGTATTTTTGAGAT
GACTGCTAAGGGTTTGATCAGTGCAAAAGATTTGGCAAATCGTTTTTTTACTCGTGGAAAGGCTATTTCAGGAAGTGCTT
TAGGTGTTGTGATGGAAGGATCTCGTGCCTTGGTTTTAGAAGTTCAAGCTTTAGTGTGTGAAAGTTCTTATCCAAAACGC
AGCGCTACAGGATATGAAAAAAATCGCTTAGATATGCTTTTGGCTTTGCTTGAAAGAAAACTTGGAATTCCTTTAGGGCA
TTATGATGTATTTGTAAATATTAGCGGCGGAGTAAAAGTAAGTGAAACTGCTGCGGATTTGGCTGTGGTTGCTGCGATTA
TTTCAAGTTTTAAAAATCGCCCTTTGAGCAAAGATAGTATTTTTATAGGAGAGCTTAGTTTAAATGGAGAAATTAGAGAG
GTTTTTAGCCTTGATACGCGTTTAAAAGAAGCTAAAATGCAAAAATTTAAAAATGCCATTATTCCTTCTAAGCCTTTGGA
AGATATAGGGCTTAAGTGTTTTGTTGCCAAAGAACTTTCACAAGTTTTAGAATGGATGTAA

Upstream 100 bases:

>100_bases
TTCCTATTTTTTATGTAGGCGTGGGTGAGCAAATGACGGATTTGCAAGAATTTAATGCAAATGCTTACTTAGATACTTTA
TTAGATCCTATTTTTGAATA

Downstream 100 bases:

>100_bases
ATTTTCATTAAAATATAAGTAAGATTTTGCTAAAATTCCGAGTTTAGAATAAAATTTTTGAGGAAACCATGAAAGATTTA
TTTTTATTTAGCTCTTTGCT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAKNKALFECQACGNQQSKWLGKCPDCGAWDSFVELKAERIKVLKELAQVSMKTSEAVCIEDVELEHFTRYSTDDNELDL
VLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQGKKVLYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEE
LHKKDYSILIVDSIQTLYSNKITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
DATKEIRLLRGFKNRFGGTNEVGIFEMTAKGLISAKDLANRFFTRGKAISGSALGVVMEGSRALVLEVQALVCESSYPKR
SATGYEKNRLDMLLALLERKLGIPLGHYDVFVNISGGVKVSETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIRE
VFSLDTRLKEAKMQKFKNAIIPSKPLEDIGLKCFVAKELSQVLEWM

Sequences:

>Translated_446_residues
MAKNKALFECQACGNQQSKWLGKCPDCGAWDSFVELKAERIKVLKELAQVSMKTSEAVCIEDVELEHFTRYSTDDNELDL
VLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQGKKVLYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEE
LHKKDYSILIVDSIQTLYSNKITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
DATKEIRLLRGFKNRFGGTNEVGIFEMTAKGLISAKDLANRFFTRGKAISGSALGVVMEGSRALVLEVQALVCESSYPKR
SATGYEKNRLDMLLALLERKLGIPLGHYDVFVNISGGVKVSETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIRE
VFSLDTRLKEAKMQKFKNAIIPSKPLEDIGLKCFVAKELSQVLEWM
>Mature_445_residues
AKNKALFECQACGNQQSKWLGKCPDCGAWDSFVELKAERIKVLKELAQVSMKTSEAVCIEDVELEHFTRYSTDDNELDLV
LGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQGKKVLYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEEL
HKKDYSILIVDSIQTLYSNKITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEGD
ATKEIRLLRGFKNRFGGTNEVGIFEMTAKGLISAKDLANRFFTRGKAISGSALGVVMEGSRALVLEVQALVCESSYPKRS
ATGYEKNRLDMLLALLERKLGIPLGHYDVFVNISGGVKVSETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIREV
FSLDTRLKEAKMQKFKNAIIPSKPLEDIGLKCFVAKELSQVLEWM

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=455, Percent_Identity=42.1978021978022, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48996; Mature: 48864

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKALFECQACGNQQSKWLGKCPDCGAWDSFVELKAERIKVLKELAQVSMKTSEAVCI
CCCCCCEEEEHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
EDVELEHFTRYSTDDNELDLVLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQGKKV
ECCCHHHHHCCCCCCCCEEEEEECCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCEE
LYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEELHKKDYSILIVDSIQTLYSN
EEEECCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHC
KITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHEEEEECC
DATKEIRLLRGFKNRFGGTNEVGIFEMTAKGLISAKDLANRFFTRGKAISGSALGVVMEG
CCCHHHHHHHHHHHHCCCCCCCEEEEECHHHCHHHHHHHHHHHHCCCCCCCCEEEEEEEC
SRALVLEVQALVCESSYPKRSATGYEKNRLDMLLALLERKLGIPLGHYDVFVNISGGVKV
CCEEEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEE
SETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIREVFSLDTRLKEAKMQKFKNAI
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
IPSKPLEDIGLKCFVAKELSQVLEWM
CCCCCHHHHCCEEEHHHHHHHHHHCC
>Mature Secondary Structure 
AKNKALFECQACGNQQSKWLGKCPDCGAWDSFVELKAERIKVLKELAQVSMKTSEAVCI
CCCCCEEEEHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
EDVELEHFTRYSTDDNELDLVLGGGLVEGSLVLIGGSPGVGKSTLLLKIASNLAKQGKKV
ECCCHHHHHCCCCCCCCEEEEEECCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCEE
LYVSGEESKAQIKLRADRLEANTPNLFLLTELCLENILEELHKKDYSILIVDSIQTLYSN
EEEECCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHC
KITSAAGSITQVREITFELMRVSKAYNISTFIIGHITKEGAIAGPRVLEHMVDVVLYFEG
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHEEEEECC
DATKEIRLLRGFKNRFGGTNEVGIFEMTAKGLISAKDLANRFFTRGKAISGSALGVVMEG
CCCHHHHHHHHHHHHCCCCCCCEEEEECHHHCHHHHHHHHHHHHCCCCCCCCEEEEEEEC
SRALVLEVQALVCESSYPKRSATGYEKNRLDMLLALLERKLGIPLGHYDVFVNISGGVKV
CCEEEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEE
SETAADLAVVAAIISSFKNRPLSKDSIFIGELSLNGEIREVFSLDTRLKEAKMQKFKNAI
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
IPSKPLEDIGLKCFVAKELSQVLEWM
CCCCCHHHHCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]