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The map label for this gene is pyrB [H]

Identifier: 57237980

GI number: 57237980

Start: 1158534

End: 1159421

Strand: Direct

Name: pyrB [H]

Synonym: CJE1241

Alternate gene names: 57237980

Gene position: 1158534-1159421 (Clockwise)

Preceding gene: 57237979

Following gene: 57237981

Centisome position: 65.17

GC content: 34.12

Gene sequence:

>888_bases
ATGCGTCATCTCATCACTACAAAGGATTTTAATAAAGTCGAAATTATGGAGCTTTTTAAAGAAGCAAGTGATTTCTTAGA
TGAAAAACCTAGGACTTTTCTTGAAGGAAAAAGCATCACGACAATTTTTTTTGAAAACTCTACACGCACCCTTTCATCTT
TTGAAAGCGCTGCAAGAAGATTAGGTGCTAGGGTTTTACGCCTTGATGTTTCAAGATCAAGCTCTAGCAAAGGAGAAACT
CTTTATGATACTGCTGCTAATTTAGATGCCATGAGTCCTAATGCTATTGTTGTAAGACACGCAAACTCAGGAGTTCCTTT
GATATTAGCAAAGCATATGCATTGTCCAGTTGTTAATGGAGGAGATGGAAAACACGCCCATCCTACCCAAGCTTTACTCG
ATTTGTTTACGATTTATAATCATTTTCAAGGTAATGTTGAAGGTAAGAAAATTTGCATCGTAGGTGATATTAAAAACTCT
CGTGTAGCAGCGTCCAATATAGAGCTTTTAAGTAGATTTAATCTTGATATTACCCTAGTAGCTCCACCACATTTTATGCC
TAATACCCATCTTAAAAAACATTATAAGCTTGATGAGAATATCATAGCAAATTCAGATATTATCATGAGTCTTAGAACAC
AAACAGAAAGACATAATAAAACCGTTTATGCTTCACTTAAAGACTATGCAAATGATTTTTGCATTCAAAAAAGCTTAGCA
AAAGATAAAAAGCTTATTTTACTACATCCAGGCCCTGTAAATCGCAATATTGACATAAGCGATGAAATGATGAGCGATGA
AAGGACTTTAGTTTTAAAACAAGTTAAAAATGGAGTGGCCATTAGAATGGCTGTTCTTAAAAAACTCATATTAGAAAATG
AAGGATAA

Upstream 100 bases:

>100_bases
AGATAGTGTTGAAACTGCTTTAAATAGTTCTACAGATGTTCTTTTTACAGCAATTTGTTCTAAAAATGAATTAAATTACA
ATATCAAATAAGGTAAAAAC

Downstream 100 bases:

>100_bases
AAAATGTTAGAATGGGATTTAAGTGCTTTATTTCACGATAAAGAAGCGTTGCAAAATTTTACACAAGATCAAATCCAACA
AAGTTTAAATTTCAAAAAAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRHLITTKDFNKVEIMELFKEASDFLDEKPRTFLEGKSITTIFFENSTRTLSSFESAARRLGARVLRLDVSRSSSSKGET
LYDTAANLDAMSPNAIVVRHANSGVPLILAKHMHCPVVNGGDGKHAHPTQALLDLFTIYNHFQGNVEGKKICIVGDIKNS
RVAASNIELLSRFNLDITLVAPPHFMPNTHLKKHYKLDENIIANSDIIMSLRTQTERHNKTVYASLKDYANDFCIQKSLA
KDKKLILLHPGPVNRNIDISDEMMSDERTLVLKQVKNGVAIRMAVLKKLILENEG

Sequences:

>Translated_295_residues
MRHLITTKDFNKVEIMELFKEASDFLDEKPRTFLEGKSITTIFFENSTRTLSSFESAARRLGARVLRLDVSRSSSSKGET
LYDTAANLDAMSPNAIVVRHANSGVPLILAKHMHCPVVNGGDGKHAHPTQALLDLFTIYNHFQGNVEGKKICIVGDIKNS
RVAASNIELLSRFNLDITLVAPPHFMPNTHLKKHYKLDENIIANSDIIMSLRTQTERHNKTVYASLKDYANDFCIQKSLA
KDKKLILLHPGPVNRNIDISDEMMSDERTLVLKQVKNGVAIRMAVLKKLILENEG
>Mature_295_residues
MRHLITTKDFNKVEIMELFKEASDFLDEKPRTFLEGKSITTIFFENSTRTLSSFESAARRLGARVLRLDVSRSSSSKGET
LYDTAANLDAMSPNAIVVRHANSGVPLILAKHMHCPVVNGGDGKHAHPTQALLDLFTIYNHFQGNVEGKKICIVGDIKNS
RVAASNIELLSRFNLDITLVAPPHFMPNTHLKKHYKLDENIIANSDIIMSLRTQTERHNKTVYASLKDYANDFCIQKSLA
KDKKLILLHPGPVNRNIDISDEMMSDERTLVLKQVKNGVAIRMAVLKKLILENEG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=34.0909090909091, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=25.2396166134185, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367364, Length=302, Percent_Identity=31.4569536423841, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1790703, Length=195, Percent_Identity=34.3589743589744, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786469, Length=336, Percent_Identity=26.7857142857143, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=35.9133126934984, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=303, Percent_Identity=37.6237623762376, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=33.8709677419355, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33107; Mature: 33107

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLITTKDFNKVEIMELFKEASDFLDEKPRTFLEGKSITTIFFENSTRTLSSFESAARR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEECCCHHHHHHHHHHHH
LGARVLRLDVSRSSSSKGETLYDTAANLDAMSPNAIVVRHANSGVPLILAKHMHCPVVNG
HHHEEEEEEECCCCCCCCCEEEHHHCCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEC
GDGKHAHPTQALLDLFTIYNHFQGNVEGKKICIVGDIKNSRVAASNIELLSRFNLDITLV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCEEEEE
APPHFMPNTHLKKHYKLDENIIANSDIIMSLRTQTERHNKTVYASLKDYANDFCIQKSLA
ECCCCCCCCCHHHHHCCCCCEECCCCEEEEEEHHHHHCCCEEEEEHHHHHHHHHHHHHHC
KDKKLILLHPGPVNRNIDISDEMMSDERTLVLKQVKNGVAIRMAVLKKLILENEG
CCCEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRHLITTKDFNKVEIMELFKEASDFLDEKPRTFLEGKSITTIFFENSTRTLSSFESAARR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEECCCHHHHHHHHHHHH
LGARVLRLDVSRSSSSKGETLYDTAANLDAMSPNAIVVRHANSGVPLILAKHMHCPVVNG
HHHEEEEEEECCCCCCCCCEEEHHHCCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEC
GDGKHAHPTQALLDLFTIYNHFQGNVEGKKICIVGDIKNSRVAASNIELLSRFNLDITLV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCEEEEE
APPHFMPNTHLKKHYKLDENIIANSDIIMSLRTQTERHNKTVYASLKDYANDFCIQKSLA
ECCCCCCCCCHHHHHCCCCCEECCCCEEEEEEHHHHHCCCEEEEEHHHHHHHHHHHHHHC
KDKKLILLHPGPVNRNIDISDEMMSDERTLVLKQVKNGVAIRMAVLKKLILENEG
CCCEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA