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The map label for this gene is thiE [H]

Identifier: 57237963

GI number: 57237963

Start: 1139101

End: 1139733

Strand: Reverse

Name: thiE [H]

Synonym: CJE1224

Alternate gene names: 57237963

Gene position: 1139733-1139101 (Counterclockwise)

Preceding gene: 57237964

Following gene: 57237962

Centisome position: 64.11

GC content: 34.28

Gene sequence:

>633_bases
ATGAAAAATAAATTAGATCTTAGCCTTTATTTGGTTGCTACTAAGGGAAATAAAAGCGAGGAGTGTTTTTTAAATACTTT
AGAAAATGCTATAAAAGGCGGAGTAAGTATCATACAACTTCGTGAAAAAGAGCTTAATGCAAGAGAATTTTATAAACTAG
GCTTAAAGGTGCAAAAACTTTGCAAGTCTTATAAAATACCTTTTTTGATTAACGATAGGGTGGATATAGCTTTAGCCTTA
GATGCTGATGGGGTGCATTTAGGACAGGAAGATTTAGAAGCAAAATTAGCTAGAAAGCTTTTAGGAGATGAAAAAATCAT
AGGCTTAAGTCTTAAAAAGTTAGAACAACTTGAGTTTATACAAGGAGTAAATTATCTAGGTTGTGGAGCTATTAAGGCAA
CGCCTACTAAAGAAAGTTCGCTTTTAAGCCTTGAGCTTTTAAGTCAAATTTGTGATAAAAGCCCTATAGGGGTTGTAGCC
ATAGGTGGGATTGACAAAGAAGCACTTGTTGAGTTAAAGGGTATAAATTTAAGCGGTGTGGCTGTGGTTAGGGCGATCAT
GGATGCTAAAGATGCTTTTTTGGCAGCTAAAGAGCTAAAGCGTAAAATCTATGAAAATTTATCTCTTAAATGA

Upstream 100 bases:

>100_bases
GCTGTTAGTGAAGCTAAAGAGTATGTAAGAAATGCGATTTATTATTCACTTAATCTTGGAAAAGGTTGTGGTCCTACAAA
TCATTTTTTCAAGTTTCTAG

Downstream 100 bases:

>100_bases
AACTCCTTTTGAAGGGGTGGAAAATCTTATTTTAAATGAGATTGTTTTTTATGATTTTAGTGTGGATTTGAGTTTGTATG
ATGCTTTAATCTGCACTTCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKNKLDLSLYLVATKGNKSEECFLNTLENAIKGGVSIIQLREKELNAREFYKLGLKVQKLCKSYKIPFLINDRVDIALAL
DADGVHLGQEDLEAKLARKLLGDEKIIGLSLKKLEQLEFIQGVNYLGCGAIKATPTKESSLLSLELLSQICDKSPIGVVA
IGGIDKEALVELKGINLSGVAVVRAIMDAKDAFLAAKELKRKIYENLSLK

Sequences:

>Translated_210_residues
MKNKLDLSLYLVATKGNKSEECFLNTLENAIKGGVSIIQLREKELNAREFYKLGLKVQKLCKSYKIPFLINDRVDIALAL
DADGVHLGQEDLEAKLARKLLGDEKIIGLSLKKLEQLEFIQGVNYLGCGAIKATPTKESSLLSLELLSQICDKSPIGVVA
IGGIDKEALVELKGINLSGVAVVRAIMDAKDAFLAAKELKRKIYENLSLK
>Mature_210_residues
MKNKLDLSLYLVATKGNKSEECFLNTLENAIKGGVSIIQLREKELNAREFYKLGLKVQKLCKSYKIPFLINDRVDIALAL
DADGVHLGQEDLEAKLARKLLGDEKIIGLSLKKLEQLEFIQGVNYLGCGAIKATPTKESSLLSLELLSQICDKSPIGVVA
IGGIDKEALVELKGINLSGVAVVRAIMDAKDAFLAAKELKRKIYENLSLK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=233, Percent_Identity=33.9055793991416, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23028; Mature: 23028

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLDLSLYLVATKGNKSEECFLNTLENAIKGGVSIIQLREKELNAREFYKLGLKVQKL
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEEEHHHHCCHHHHHHHHHHHHHH
CKSYKIPFLINDRVDIALALDADGVHLGQEDLEAKLARKLLGDEKIIGLSLKKLEQLEFI
HHHCCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
QGVNYLGCGAIKATPTKESSLLSLELLSQICDKSPIGVVAIGGIDKEALVELKGINLSGV
HCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHEECCCCCHHH
AVVRAIMDAKDAFLAAKELKRKIYENLSLK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNKLDLSLYLVATKGNKSEECFLNTLENAIKGGVSIIQLREKELNAREFYKLGLKVQKL
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEEEHHHHCCHHHHHHHHHHHHHH
CKSYKIPFLINDRVDIALALDADGVHLGQEDLEAKLARKLLGDEKIIGLSLKKLEQLEFI
HHHCCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
QGVNYLGCGAIKATPTKESSLLSLELLSQICDKSPIGVVAIGGIDKEALVELKGINLSGV
HCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHEECCCCCHHH
AVVRAIMDAKDAFLAAKELKRKIYENLSLK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA