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The map label for this gene is rpoD [H]

Identifier: 57237826

GI number: 57237826

Start: 1008679

End: 1010547

Strand: Direct

Name: rpoD [H]

Synonym: CJE1081

Alternate gene names: 57237826

Gene position: 1008679-1010547 (Clockwise)

Preceding gene: 57237825

Following gene: 57237829

Centisome position: 56.74

GC content: 30.77

Gene sequence:

>1869_bases
ATGAACGCCAAAACTCAAGAAGCTGAATTAGAAGAATTATTTCAAGAAAATGCAAAAGATTATATCACATATGAAAAATT
GGTAAAATATCTTACAAAACAACCAAGTGCATCAACCGCTAAAAAAGTGCAAGCATTAATGAAAAAACACAAAGTGCAAC
TTTTTTCAGCAGCTGAAATTGCACAAATGAAAAACATAGAAGATGCAAAAAGACTTCAAGAAGAAAAACAAAAACTCCAA
GATACAAGCCTTGAGAACGAATTTGATTTAGCCAATGAAAACGATTTATTAGAATGGAGCAGATCAGACTCTCCTGTTCG
TATGTATTTAAGAGAAATGGGACAAATTGCTTTATTAAACAAAGATGAAGAAATTGAAATTTCCAAAAAAATAGAACTTG
GCGAAGATATTATCATTGATGCTTTTTGTTCTGTACCTTATTTGATAGATTTTATTTTAGATTATAAAGAACCTTTAATA
AACAGAGAAAGAAGGGTAAAAGAACTATTTAAAAGCTTTGACGATGAAGAAAAAAGTGATGATAAACTTGATGAACTTGA
TATAGATGAAGATGAAGAAAACGAAACAGAATTAGAATCAGATGAAGAAAATACTAAAAAAAACAGCAAAAAAGAAGATG
AAAGAACTTTAAAAGTTATAGAAAAATTTAAAGCTCTAGAAAAAGCTAAAAAAGATTGGCTAAAAACATCCAAAGACAAA
GAAAGCGGAGATGAACTTTTAGATAAATTAAGCATTGCGTTCAAAAAAAATATTTTAAAAGAAAAATTAATGGATCTAGG
CCCTACTTCAAAACTTATTAGCGAAATCGTAAAATCAATGGAAACTGCACTAAAAAGTGATGAAGAATTTGACAAAGAAC
TTAAGCGTTTAGAATACCGCTTACCTATGTTTTCTGATGAACTTAAAAAACGCCATGCAGATATTTTAAAAGATATTACA
AAATTGAGCAAAGAAGAAATCACAGAGCGAGCTTTAGAAACAACCATGGTAAGCACTTATATGGAAATTAAAAAACTATT
TCAAACTAAAGAAGCCAGTGAAAAAAGCTTTGATCTTGAAAAATCACGCTTAAAAGAAATTTTGGAGCAAATTAAACGCG
GTAAAAAAATCTCTGATGAAGCAAAAGGAAGAATGGCAAAATCAAACTTGCGCCTTGTTGTAAGCATAGCTAAACGCTAC
ACAAATCGTGGTCTGCCTTTCTTAGACCTTATCCAAGAAGGAAATATAGGTTTGATGAAAGCTGTGGATAAATTTGAATA
CAAAAGGGGTTATAAATTTTCCACTTATGCAACTTGGTGGATCAGGCAAGCTATTTCAAGGGCTATAGCTGATCAAGCAA
GAACCATAAGAATTCCAATCCATATGATAGAAACCATCAATCAAATTAACAAAATTATCCGCGAACATCTACAAAAAGAT
GGAAAAGAACCTGATGTCAGTGTTATAGCTAAAGAAGTAGGTTTAAGCGTAGATAAGGTTAAACAAGTTATTAAAATCAC
AAAAGAACCAATTTCTCTTGAAGCTCCAATAGGAAATGAAGATGATGGTAAATTTGGAGATTTTGTTGAAGATAGAAATT
CACTTTCTCCAATGGATCATATCTTAAAGGATGATTTAAAAGAACAAATTGATGAAGTTTTAGATCAATTAAACGATAGA
GAAAAAGCGGTAATTCGTATGCGTTTTGGTTTAATGGATGATGAAAGTGATAGAACTTTAGAGGAAATTGGAAAGGAACT
TAATGTCACTCGAGAAAGAGTGAGACAAATAGAAAGCTCAGCTATAAAAAAACTCAAACACCCTAAAGTAGGAAGAAAAC
TAAAAAATTATATAGAAGGTTGGAAGTAA

Upstream 100 bases:

>100_bases
GAAAAAATATTCAAATCATAAACATAATAACCGATTTATATAGAAAGTTAAGCAATTTTTAGATAAAATCCAAAGCTTAT
ATAAATTTCAAGGATAATCA

Downstream 100 bases:

>100_bases
GAGTCAATCTCCTTACTAAAAACCCAGATCTTTTTCTTCTTTTAACTTGCGTACATGTTCAAAAAAAACTAATTTTCCGC
TATGTTTTTTTAAATTTTTA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNAKTQEAELEELFQENAKDYITYEKLVKYLTKQPSASTAKKVQALMKKHKVQLFSAAEIAQMKNIEDAKRLQEEKQKLQ
DTSLENEFDLANENDLLEWSRSDSPVRMYLREMGQIALLNKDEEIEISKKIELGEDIIIDAFCSVPYLIDFILDYKEPLI
NRERRVKELFKSFDDEEKSDDKLDELDIDEDEENETELESDEENTKKNSKKEDERTLKVIEKFKALEKAKKDWLKTSKDK
ESGDELLDKLSIAFKKNILKEKLMDLGPTSKLISEIVKSMETALKSDEEFDKELKRLEYRLPMFSDELKKRHADILKDIT
KLSKEEITERALETTMVSTYMEIKKLFQTKEASEKSFDLEKSRLKEILEQIKRGKKISDEAKGRMAKSNLRLVVSIAKRY
TNRGLPFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAISRAIADQARTIRIPIHMIETINQINKIIREHLQKD
GKEPDVSVIAKEVGLSVDKVKQVIKITKEPISLEAPIGNEDDGKFGDFVEDRNSLSPMDHILKDDLKEQIDEVLDQLNDR
EKAVIRMRFGLMDDESDRTLEEIGKELNVTRERVRQIESSAIKKLKHPKVGRKLKNYIEGWK

Sequences:

>Translated_622_residues
MNAKTQEAELEELFQENAKDYITYEKLVKYLTKQPSASTAKKVQALMKKHKVQLFSAAEIAQMKNIEDAKRLQEEKQKLQ
DTSLENEFDLANENDLLEWSRSDSPVRMYLREMGQIALLNKDEEIEISKKIELGEDIIIDAFCSVPYLIDFILDYKEPLI
NRERRVKELFKSFDDEEKSDDKLDELDIDEDEENETELESDEENTKKNSKKEDERTLKVIEKFKALEKAKKDWLKTSKDK
ESGDELLDKLSIAFKKNILKEKLMDLGPTSKLISEIVKSMETALKSDEEFDKELKRLEYRLPMFSDELKKRHADILKDIT
KLSKEEITERALETTMVSTYMEIKKLFQTKEASEKSFDLEKSRLKEILEQIKRGKKISDEAKGRMAKSNLRLVVSIAKRY
TNRGLPFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAISRAIADQARTIRIPIHMIETINQINKIIREHLQKD
GKEPDVSVIAKEVGLSVDKVKQVIKITKEPISLEAPIGNEDDGKFGDFVEDRNSLSPMDHILKDDLKEQIDEVLDQLNDR
EKAVIRMRFGLMDDESDRTLEEIGKELNVTRERVRQIESSAIKKLKHPKVGRKLKNYIEGWK
>Mature_622_residues
MNAKTQEAELEELFQENAKDYITYEKLVKYLTKQPSASTAKKVQALMKKHKVQLFSAAEIAQMKNIEDAKRLQEEKQKLQ
DTSLENEFDLANENDLLEWSRSDSPVRMYLREMGQIALLNKDEEIEISKKIELGEDIIIDAFCSVPYLIDFILDYKEPLI
NRERRVKELFKSFDDEEKSDDKLDELDIDEDEENETELESDEENTKKNSKKEDERTLKVIEKFKALEKAKKDWLKTSKDK
ESGDELLDKLSIAFKKNILKEKLMDLGPTSKLISEIVKSMETALKSDEEFDKELKRLEYRLPMFSDELKKRHADILKDIT
KLSKEEITERALETTMVSTYMEIKKLFQTKEASEKSFDLEKSRLKEILEQIKRGKKISDEAKGRMAKSNLRLVVSIAKRY
TNRGLPFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAISRAIADQARTIRIPIHMIETINQINKIIREHLQKD
GKEPDVSVIAKEVGLSVDKVKQVIKITKEPISLEAPIGNEDDGKFGDFVEDRNSLSPMDHILKDDLKEQIDEVLDQLNDR
EKAVIRMRFGLMDDESDRTLEEIGKELNVTRERVRQIESSAIKKLKHPKVGRKLKNYIEGWK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=44.2477876106195, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1789871, Length=244, Percent_Identity=29.0983606557377, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=27.1844660194175, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 72577; Mature: 72577

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKTQEAELEELFQENAKDYITYEKLVKYLTKQPSASTAKKVQALMKKHKVQLFSAAEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AQMKNIEDAKRLQEEKQKLQDTSLENEFDLANENDLLEWSRSDSPVRMYLREMGQIALLN
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCEEECC
KDEEIEISKKIELGEDIIIDAFCSVPYLIDFILDYKEPLINRERRVKELFKSFDDEEKSD
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DKLDELDIDEDEENETELESDEENTKKNSKKEDERTLKVIEKFKALEKAKKDWLKTSKDK
CCHHCCCCCCCCCCCHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESGDELLDKLSIAFKKNILKEKLMDLGPTSKLISEIVKSMETALKSDEEFDKELKRLEYR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LPMFSDELKKRHADILKDITKLSKEEITERALETTMVSTYMEIKKLFQTKEASEKSFDLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KSRLKEILEQIKRGKKISDEAKGRMAKSNLRLVVSIAKRYTNRGLPFLDLIQEGNIGLMK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHH
AVDKFEYKRGYKFSTYATWWIRQAISRAIADQARTIRIPIHMIETINQINKIIREHLQKD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
GKEPDVSVIAKEVGLSVDKVKQVIKITKEPISLEAPIGNEDDGKFGDFVEDRNSLSPMDH
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHCCCCCHHHH
ILKDDLKEQIDEVLDQLNDREKAVIRMRFGLMDDESDRTLEEIGKELNVTRERVRQIESS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AIKKLKHPKVGRKLKNYIEGWK
HHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAKTQEAELEELFQENAKDYITYEKLVKYLTKQPSASTAKKVQALMKKHKVQLFSAAEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AQMKNIEDAKRLQEEKQKLQDTSLENEFDLANENDLLEWSRSDSPVRMYLREMGQIALLN
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCEEECC
KDEEIEISKKIELGEDIIIDAFCSVPYLIDFILDYKEPLINRERRVKELFKSFDDEEKSD
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DKLDELDIDEDEENETELESDEENTKKNSKKEDERTLKVIEKFKALEKAKKDWLKTSKDK
CCHHCCCCCCCCCCCHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESGDELLDKLSIAFKKNILKEKLMDLGPTSKLISEIVKSMETALKSDEEFDKELKRLEYR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LPMFSDELKKRHADILKDITKLSKEEITERALETTMVSTYMEIKKLFQTKEASEKSFDLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KSRLKEILEQIKRGKKISDEAKGRMAKSNLRLVVSIAKRYTNRGLPFLDLIQEGNIGLMK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHH
AVDKFEYKRGYKFSTYATWWIRQAISRAIADQARTIRIPIHMIETINQINKIIREHLQKD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
GKEPDVSVIAKEVGLSVDKVKQVIKITKEPISLEAPIGNEDDGKFGDFVEDRNSLSPMDH
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHCCCCCHHHH
ILKDDLKEQIDEVLDQLNDREKAVIRMRFGLMDDESDRTLEEIGKELNVTRERVRQIESS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AIKKLKHPKVGRKLKNYIEGWK
HHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]