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The map label for this gene is trmE [H]

Identifier: 57237784

GI number: 57237784

Start: 975176

End: 976504

Strand: Reverse

Name: trmE [H]

Synonym: CJE1036

Alternate gene names: 57237784

Gene position: 976504-975176 (Counterclockwise)

Preceding gene: 57237785

Following gene: 57237783

Centisome position: 54.93

GC content: 30.93

Gene sequence:

>1329_bases
ATGAGTGATACTATAGCGGCTATTGCCACAGCTCATGGTGTGGGTTCGATTTCTATTGTAAGATTAAGCGGTGAAAGGGC
TTTAGAATTTGCCTTAAAGCTTAGCCACAAAACCAAACTTACCCCACGCCATGCTACTTTTACAAAACTTTTTAATCAAA
ATAATGAAATTATCGATGAAGCTATAATGATATATTTTAAAGCCCCTTATAGTTTTACAGGGGAAGATATAGTAGAGTTT
CAAATTCATGGAGGATTTAGTGTGAGTGAAGTTTTACTCGAAGAACTTGTGAGTTTAGGAGCAAGACTTGCTTTAGCAGG
GGAATTTAGCAAAAGAGCGTGTTTAAATGGTAAAATGACTCCTTTAAAAGCTTTAAATATACAAGATCTCATACTTTCAA
AATCTGCACTTGCAGCTAAAATTATAGCACGCAACATGCAAGGAAACTTAGGAGAACTTTTAGAAAAAATTCGCACAGAT
TTGGTTAAAACTTTAGCTTTTGTAGAAACTAGTATTGATTATGCTGATGATGATTTACCTTCTGATTTATTAGAACAAAT
TTCTACTATGTGTGAAGAAAATTCTAAAATTTTAAAAGAAATTTATACGCTTTCTCAAAGCAAAAAAGGACTCATAGAAG
GTTTTAAAATCGCTATTGTAGGTAAGCCAAATGTAGGCAAATCTTCGCTTTTAAATGCCTTGCTTTCTTATGAAAGAGCT
ATTGTTTCAGATATAGCAGGCACCACTCGTGATACTATAGAAGAGAGCTTTAAATTAGGAACGCATTTATTACGCATTAT
AGATACAGCGGGTATTAGGGAAAGTAAAGATGTTATAGAACAAATCGGCGTAGCTTTAAGTAAAAAAAGTTTAGAAGATG
CAGATATTATTTTGGCAGTCTTTGATGCTTCAAGAGTGCAAGATAAAGAAGATGAGAAAATTTTTGACTTATTAGCAAAT
ACTGATAAAAAAATCTTTTGGATTTTAAATAAAAGTGATTTAGAAAATGTTTTCAAAAATACTCAAAATAAAAATTTTAT
CAAGCTTAGTGCTCAAAAAGACATCACTTTGTTAAAAGAAGAATTGCAAAATTATCTTAATTCTTTTGATAGTGAAGGTA
TTATGGTAAGTTCTTTAGATCTTATTAATGCTTGTAAAATTTCAAGTGAAGCGATTTTTAGAGCTAAGGGACTTTTAGAG
GAAAGTTCTTTAGAACTTTTTGCTTTTGAACTTAATTTAGCGATTAATGAACTTGCAAGATTTACCAAAGATTTTCAAAG
AGATGAAATTTTAGACGAAATGTTTGGTAATTTTTGTTTAGGAAAATGA

Upstream 100 bases:

>100_bases
GCTTTAGAGCAGTTAAGAGCTGAATTTCCAGATAAGTATGTAGGAATTAAGCAAAATAATGATCAAAGATTTGTTGTAAT
AAACGACTTTTTCAAAAAAG

Downstream 100 bases:

>100_bases
AAGGATAAAAATGGACAAAGAAACCATAAAAGCACACAAAATTAGCGATGAAGAATATACACAAATTTTAGAAATTTTAG
GACGTGAGCCAAATTTATTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSDTIAAIATAHGVGSISIVRLSGERALEFALKLSHKTKLTPRHATFTKLFNQNNEIIDEAIMIYFKAPYSFTGEDIVEF
QIHGGFSVSEVLLEELVSLGARLALAGEFSKRACLNGKMTPLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTD
LVKTLAFVETSIDYADDDLPSDLLEQISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIVGKPNVGKSSLLNALLSYERA
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDVIEQIGVALSKKSLEDADIILAVFDASRVQDKEDEKIFDLLAN
TDKKIFWILNKSDLENVFKNTQNKNFIKLSAQKDITLLKEELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKGLLE
ESSLELFAFELNLAINELARFTKDFQRDEILDEMFGNFCLGK

Sequences:

>Translated_442_residues
MSDTIAAIATAHGVGSISIVRLSGERALEFALKLSHKTKLTPRHATFTKLFNQNNEIIDEAIMIYFKAPYSFTGEDIVEF
QIHGGFSVSEVLLEELVSLGARLALAGEFSKRACLNGKMTPLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTD
LVKTLAFVETSIDYADDDLPSDLLEQISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIVGKPNVGKSSLLNALLSYERA
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDVIEQIGVALSKKSLEDADIILAVFDASRVQDKEDEKIFDLLAN
TDKKIFWILNKSDLENVFKNTQNKNFIKLSAQKDITLLKEELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKGLLE
ESSLELFAFELNLAINELARFTKDFQRDEILDEMFGNFCLGK
>Mature_441_residues
SDTIAAIATAHGVGSISIVRLSGERALEFALKLSHKTKLTPRHATFTKLFNQNNEIIDEAIMIYFKAPYSFTGEDIVEFQ
IHGGFSVSEVLLEELVSLGARLALAGEFSKRACLNGKMTPLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTDL
VKTLAFVETSIDYADDDLPSDLLEQISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIVGKPNVGKSSLLNALLSYERAI
VSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDVIEQIGVALSKKSLEDADIILAVFDASRVQDKEDEKIFDLLANT
DKKIFWILNKSDLENVFKNTQNKNFIKLSAQKDITLLKEELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKGLLEE
SSLELFAFELNLAINELARFTKDFQRDEILDEMFGNFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=32.3275862068966, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=30.8467741935484, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=31.6483516483516, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=34.2857142857143, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082120, Length=121, Percent_Identity=36.3636363636364, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=450, Percent_Identity=29.1111111111111, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=30.7535641547862, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=27.906976744186, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49096; Mature: 48965

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTIAAIATAHGVGSISIVRLSGERALEFALKLSHKTKLTPRHATFTKLFNQNNEIIDE
CCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHH
AIMIYFKAPYSFTGEDIVEFQIHGGFSVSEVLLEELVSLGARLALAGEFSKRACLNGKMT
HHHEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCHHEEECCCHHHHHHCCCCCC
PLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTDLVKTLAFVETSIDYADDDLP
CHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SDLLEQISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIVGKPNVGKSSLLNALLSYERA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDVIEQIGVALSKKSLEDADIILAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
FDASRVQDKEDEKIFDLLANTDKKIFWILNKSDLENVFKNTQNKNFIKLSAQKDITLLKE
ECCHHCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
ELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKGLLEESSLELFAFELNLAINELAR
HHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHH
FTKDFQRDEILDEMFGNFCLGK
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDTIAAIATAHGVGSISIVRLSGERALEFALKLSHKTKLTPRHATFTKLFNQNNEIIDE
CCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHH
AIMIYFKAPYSFTGEDIVEFQIHGGFSVSEVLLEELVSLGARLALAGEFSKRACLNGKMT
HHHEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCHHEEECCCHHHHHHCCCCCC
PLKALNIQDLILSKSALAAKIIARNMQGNLGELLEKIRTDLVKTLAFVETSIDYADDDLP
CHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SDLLEQISTMCEENSKILKEIYTLSQSKKGLIEGFKIAIVGKPNVGKSSLLNALLSYERA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
IVSDIAGTTRDTIEESFKLGTHLLRIIDTAGIRESKDVIEQIGVALSKKSLEDADIILAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
FDASRVQDKEDEKIFDLLANTDKKIFWILNKSDLENVFKNTQNKNFIKLSAQKDITLLKE
ECCHHCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCHHHHHHH
ELQNYLNSFDSEGIMVSSLDLINACKISSEAIFRAKGLLEESSLELFAFELNLAINELAR
HHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHH
FTKDFQRDEILDEMFGNFCLGK
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA