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The map label for this gene is nadE [H]

Identifier: 57237655

GI number: 57237655

Start: 839868

End: 840608

Strand: Direct

Name: nadE [H]

Synonym: CJE0901

Alternate gene names: 57237655

Gene position: 839868-840608 (Clockwise)

Preceding gene: 57237652

Following gene: 57237656

Centisome position: 47.24

GC content: 32.12

Gene sequence:

>741_bases
ATGGATTGGCAAAAGATTACGGAAAAAATGTGTGATTTTATACAAGAAAAAGTGAAAAATTCTCAAAGTCAAGGTGTGGT
TTTGGGGCTTAGTGGCGGGATTGATTCTGCTTTGGTAGCAACGCTTTGTAAAAGAGCTTTAAAAGAGAATGTTTTTGCTC
TTTTAATGCCAACTCAAATTTCAAACAAAGCCAATCTTGAAGATGCTTTAAGGCTTTGTGCGGATTTAAATCTTGAATAT
AAAATCATAGAAATTCAAAGCATTTTAGATGCTTTTATAAAGCAAAGTGAAAATACCACTCTAGTATCTTTGGGAAATTT
TGCAGCGCGTATTAGAATGAGTTTGCTTTATGATTATTCTGCTTTGAAAAATTCTTTAGTCATAGGCACTTCTAATAAAA
GCGAATTGCTTTTAGGTTATGGTACGATTTATGGGGATTTAGCTTGTGCTTTTAATCCTATAGGATCGCTTTATAAAAGT
GAAATTTATGCCTTAGCAAAATACCTTAACTTGCATGAAAATTTCATCAAAAAAGCTCCAAGTGCGGATCTTTGGGAAAA
TCAAAGTGATGAGGCGGATTTAGGTTTTTCTTATGCAAAAATCGATGAAGGTTTAAAAGCTTTAGAAACTAATGATGAAA
AACTTTTAAGAACGCTTGATCCTTCTTTGATTGCTATGTTAAAAAATCGCATGCAAAAAAATGCTTTTAAGGGAAAAATG
CCTGAAATTTTGGAAATTTAA

Upstream 100 bases:

>100_bases
TCCACAAGCTTGTTTGATAATTTGCATTTTTTGCCTCTTTAATAAAAAATTTGCCTTAAATAAATATAATTATACATTAA
ATTTTTAAAAGGCAAGTTTT

Downstream 100 bases:

>100_bases
ACAATGAATGAAGAAAAAAATTATGAACTTTGGCTTGATAATTATTTTTTTAAGCCCAATTTTTGGCAAAAATGCCTTGC
TTTTATACTTTTACCTTTAA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRALKENVFALLMPTQISNKANLEDALRLCADLNLEY
KIIEIQSILDAFIKQSENTTLVSLGNFAARIRMSLLYDYSALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKS
EIYALAKYLNLHENFIKKAPSADLWENQSDEADLGFSYAKIDEGLKALETNDEKLLRTLDPSLIAMLKNRMQKNAFKGKM
PEILEI

Sequences:

>Translated_246_residues
MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRALKENVFALLMPTQISNKANLEDALRLCADLNLEY
KIIEIQSILDAFIKQSENTTLVSLGNFAARIRMSLLYDYSALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKS
EIYALAKYLNLHENFIKKAPSADLWENQSDEADLGFSYAKIDEGLKALETNDEKLLRTLDPSLIAMLKNRMQKNAFKGKM
PEILEI
>Mature_246_residues
MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRALKENVFALLMPTQISNKANLEDALRLCADLNLEY
KIIEIQSILDAFIKQSENTTLVSLGNFAARIRMSLLYDYSALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKS
EIYALAKYLNLHENFIKKAPSADLWENQSDEADLGFSYAKIDEGLKALETNDEKLLRTLDPSLIAMLKNRMQKNAFKGKM
PEILEI

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=215, Percent_Identity=28.8372093023256, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 27433; Mature: 27433

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRALKENVFALLMPTQI
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCC
SNKANLEDALRLCADLNLEYKIIEIQSILDAFIKQSENTTLVSLGNFAARIRMSLLYDYS
CCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
ALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKSEIYALAKYLNLHENFIKKAP
HHHCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SADLWENQSDEADLGFSYAKIDEGLKALETNDEKLLRTLDPSLIAMLKNRMQKNAFKGKM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
PEILEI
CHHHCC
>Mature Secondary Structure
MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRALKENVFALLMPTQI
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCC
SNKANLEDALRLCADLNLEYKIIEIQSILDAFIKQSENTTLVSLGNFAARIRMSLLYDYS
CCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
ALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKSEIYALAKYLNLHENFIKKAP
HHHCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SADLWENQSDEADLGFSYAKIDEGLKALETNDEKLLRTLDPSLIAMLKNRMQKNAFKGKM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
PEILEI
CHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA