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The map label for this gene is cofH [H]

Identifier: 55980773

GI number: 55980773

Start: 768345

End: 769463

Strand: Reverse

Name: cofH [H]

Synonym: TTHA0804

Alternate gene names: 55980773

Gene position: 769463-768345 (Counterclockwise)

Preceding gene: 55980774

Following gene: 55980772

Centisome position: 41.6

GC content: 66.4

Gene sequence:

>1119_bases
GTGAGAGGCATCCGGGACCCCCGCCTCATCCCCATCGCCGAGAAGGTGATGGAAGGGAAAAGGCTTTCCTTTGAGGACGG
CCTCGTCCTCTACCAGACCAAGGACCTTCCCACCCTCATGCGCCTCGCCAACCTGGTCCGCGAGCGGAAGCACGGGCACA
AGACCTACTTCGTCCACTCCATCCGCGTCTCCCAGACCAACATCTGCTACGTGGGGTGCACCTTCTGCGCCTTCCAAAGG
CGCTTCGGCGAGGAAGGGGCCTGGGACTGGGACGTGGACGAGGTGGTGGCCTGGGTAAAGGAGCGCTACCAGCCGGGGCT
CACGGAAATCCACCTCACGGCGGGCCACCACCCCAAAAGGCCCTTCGCCTACTACCTGGACCTGGTACGGGCCCTGAAGG
AGAACTTCCCCGGGGTCCAGGTGAAGGCCTGGACCGCGGCGGAGATCCACCACTTCTCCAAGATCGCCCGCCTCCCCTAC
CGGGAGGTCCTAAAGGCCCTAAAGGAGGCGGGCCTGGACGCCATGCCGGGAGGCGGGGCGGAGATCTTCGCCGAAAGGGT
GCGCAGGAAGATCGCCCGGGCCAAGGTCTCGGCGGAAGGGTGGCTGGAGATCCACCGCACCGCCCACGAGCTTGGCATCC
CCACCAACGCCACCATGCTCTACGGGCACATAGAAACCCTGGAGGAACGCCTGGACCACATGGACCGCCTCCGCCGCCTC
CAGGACGAGACCGGGGGCTTCATGAGCTTCATCCCCCTGGCCTTCCAGCCGGACGGGAACCAGCTCGCCCGGGAGCTGGG
CAAGAAGGAGTTCACCACGGGGCTGGACGACCTTAGAAACCTGGCCGTGGCCCGGCTTTACCTGGACAACTTCCCCCACA
TCAAGGGGTACTGGGCCACCTTAACCCCCGAGCTCGCCCAGGTCTCCTTGGACTGGGGGGTCACGGACGTGGACGGCACC
CTGATTGAGGAGAGGATCGTCCACATGGCGGGAAGCCCCACGCCCCAGGGCCTCACCAAGCGGGAGCTCGCCCGCATCAT
CCTCATGGCGGGGCGGATCCCCGTGGAGCGGGACGCCCTCTACCGGGAGGTGCGGGTCTGGGACCGGGTGGAGGCGTGA

Upstream 100 bases:

>100_bases
CGCTACCTGAGGGGCCTCCTCCCCGAGAAGAGCGTGGGGGGGCGGAGGAAGGAGGTGGAGGCCCTGGTCAGGCGCCTTGA
AGGGGAGTAGACTCCGGGGC

Downstream 100 bases:

>100_bases
GGCCCTACGTCCTGGGCCTTCCCCGCTACGCCAACGTCGCCCCCCTCCACCACTTCCTGAGGCTCGAGGGCTTCCGGGTT
CTCCACGCCGTCCCCGCAGA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRGIRDPRLIPIAEKVMEGKRLSFEDGLVLYQTKDLPTLMRLANLVRERKHGHKTYFVHSIRVSQTNICYVGCTFCAFQR
RFGEEGAWDWDVDEVVAWVKERYQPGLTEIHLTAGHHPKRPFAYYLDLVRALKENFPGVQVKAWTAAEIHHFSKIARLPY
REVLKALKEAGLDAMPGGGAEIFAERVRRKIARAKVSAEGWLEIHRTAHELGIPTNATMLYGHIETLEERLDHMDRLRRL
QDETGGFMSFIPLAFQPDGNQLARELGKKEFTTGLDDLRNLAVARLYLDNFPHIKGYWATLTPELAQVSLDWGVTDVDGT
LIEERIVHMAGSPTPQGLTKRELARIILMAGRIPVERDALYREVRVWDRVEA

Sequences:

>Translated_372_residues
MRGIRDPRLIPIAEKVMEGKRLSFEDGLVLYQTKDLPTLMRLANLVRERKHGHKTYFVHSIRVSQTNICYVGCTFCAFQR
RFGEEGAWDWDVDEVVAWVKERYQPGLTEIHLTAGHHPKRPFAYYLDLVRALKENFPGVQVKAWTAAEIHHFSKIARLPY
REVLKALKEAGLDAMPGGGAEIFAERVRRKIARAKVSAEGWLEIHRTAHELGIPTNATMLYGHIETLEERLDHMDRLRRL
QDETGGFMSFIPLAFQPDGNQLARELGKKEFTTGLDDLRNLAVARLYLDNFPHIKGYWATLTPELAQVSLDWGVTDVDGT
LIEERIVHMAGSPTPQGLTKRELARIILMAGRIPVERDALYREVRVWDRVEA
>Mature_372_residues
MRGIRDPRLIPIAEKVMEGKRLSFEDGLVLYQTKDLPTLMRLANLVRERKHGHKTYFVHSIRVSQTNICYVGCTFCAFQR
RFGEEGAWDWDVDEVVAWVKERYQPGLTEIHLTAGHHPKRPFAYYLDLVRALKENFPGVQVKAWTAAEIHHFSKIARLPY
REVLKALKEAGLDAMPGGGAEIFAERVRRKIARAKVSAEGWLEIHRTAHELGIPTNATMLYGHIETLEERLDHMDRLRRL
QDETGGFMSFIPLAFQPDGNQLARELGKKEFTTGLDDLRNLAVARLYLDNFPHIKGYWATLTPELAQVSLDWGVTDVDGT
LIEERIVHMAGSPTPQGLTKRELARIILMAGRIPVERDALYREVRVWDRVEA

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42614; Mature: 42614

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGIRDPRLIPIAEKVMEGKRLSFEDGLVLYQTKDLPTLMRLANLVRERKHGHKTYFVHS
CCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
IRVSQTNICYVGCTFCAFQRRFGEEGAWDWDVDEVVAWVKERYQPGLTEIHLTAGHHPKR
EEECCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
PFAYYLDLVRALKENFPGVQVKAWTAAEIHHFSKIARLPYREVLKALKEAGLDAMPGGGA
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCH
EIFAERVRRKIARAKVSAEGWLEIHRTAHELGIPTNATMLYGHIETLEERLDHMDRLRRL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
QDETGGFMSFIPLAFQPDGNQLARELGKKEFTTGLDDLRNLAVARLYLDNFPHIKGYWAT
HHHCCCCEEEECEEECCCHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LTPELAQVSLDWGVTDVDGTLIEERIVHMAGSPTPQGLTKRELARIILMAGRIPVERDAL
CCHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
YREVRVWDRVEA
HHHHHHHHHCCC
>Mature Secondary Structure
MRGIRDPRLIPIAEKVMEGKRLSFEDGLVLYQTKDLPTLMRLANLVRERKHGHKTYFVHS
CCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
IRVSQTNICYVGCTFCAFQRRFGEEGAWDWDVDEVVAWVKERYQPGLTEIHLTAGHHPKR
EEECCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
PFAYYLDLVRALKENFPGVQVKAWTAAEIHHFSKIARLPYREVLKALKEAGLDAMPGGGA
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCH
EIFAERVRRKIARAKVSAEGWLEIHRTAHELGIPTNATMLYGHIETLEERLDHMDRLRRL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
QDETGGFMSFIPLAFQPDGNQLARELGKKEFTTGLDDLRNLAVARLYLDNFPHIKGYWAT
HHHCCCCEEEECEEECCCHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
LTPELAQVSLDWGVTDVDGTLIEERIVHMAGSPTPQGLTKRELARIILMAGRIPVERDAL
CCHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
YREVRVWDRVEA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]