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The map label for this gene is pheT [H]

Identifier: 54295551

GI number: 54295551

Start: 3007455

End: 3009836

Strand: Reverse

Name: pheT [H]

Synonym: lpl2638

Alternate gene names: 54295551

Gene position: 3009836-3007455 (Counterclockwise)

Preceding gene: 54295552

Following gene: 54295550

Centisome position: 89.96

GC content: 41.35

Gene sequence:

>2382_bases
ATGAAAGTAAGTAAGTTATGGTTACGAGAATGGGTAAACTTCTCATTAACTGAACAAGAACTGGCTGAGCAATTGACCAT
GGCTGGCTTGGAAGTCGATGCAGTGAATCCTGTAGCTGGCCAATTCACTCATGTCATTGTTGCTGAAGTCCTAAATACCA
AACCTCATCCAGATGCTGATAAATTAACCTTATGTGAAGTGAATATCAATAAAGACAAGCCGTTGAAAATTGTTTGTGGT
GCGGCTAATGTGAGGCCTGGTTTGAGAGTTGCCCTGGCCATGATTGGGGCACACTTGCCTGGCGGTTTGCAAATTAAGGA
ATCCAAATTGCGAGGAGAATTGTCTCAGGGCATGTTGTGTTCTGCGACAGAACTGGGACTGGCAGAACATTCCGAGGGGA
TAATGGAACTGAGTGATGAGGCGCCAATTGGAATGGATTTACGCGAATATCTTGCCCTTGATGACCATGTATTTGATATT
GATTTGACTCCGAATCGCGCAGATTGCTTTAGTATTTTAGGGGTCGCGCGGGAAGTGGCTGTTCTCAATAAATTACCCTT
AATAGAGCAACCAATAGTTACAGTGCCACCAGCCATCGATGATGGTCTTCGAGTCAATTTGATTCATTCCGAGGCATGTC
CCCGATATTGCGGTCGAATTATCCGTAATTTAAATCTTGAAGCGAAAACGCCATTATGGATGGCTGAGCGCTTACGTAGA
GGTGGCATTAGAACGTTACACCCTGTAGTGGATGTGATGAATTATGTCATGCTCGAGCTTGGACAACCCATGCATGCTTT
TGATTTATCAAAAATTAACGGTGAAATTAATGTACGTTATAGTGCTTCAGGCGAGCAATTAGAATTACTGGATGGTCAAG
AAGTTGTATTAAACGACAATGTGTTGGTAATCGCTGATAAAGAAAAGCCTTTGGCTATGGCCGGAATAATGGGAGGAGCT
AATAGCGCAGTTCAAGAGCAGACACAACATATTTTCCTGGAAAGCGCCTATTTTAATCCGGTGACAATTGCCGGAGTTGC
CAGAAAATATGGCTTATTCAGTGATTCGTCACAACGATTCGAGCGGGGAGTGGACCCTTGTTTGCAATCAAAAGCGCTTG
AAAGAGCGACGGAATTGATTTTATCAATCTCAGGAGGTGAACCTGGGCCAGTGATAGAGTCTTTTGACAAAAAGTTTTTG
CCAGGTACGGTTTCCTTTTTATTTGATACAACAAAAGTGAAAAAGTTAACCGGGCTGTCTATCCCTCTAAACGAAATGAA
AAATTTACTGGAAGGTCTAGGTGTTGTCATCATCAAAGAAACCAATCATTTTTTTGAGGTTACCATTCCTTCTCATCGCG
TAGATTTGCAACAAGATGCTGATTTGGTTGAAGAAATTATTCGTTTATATGGCTATGACAAGTTACAAGCTCAGCCAATG
CAAACATCAGTACAAGCTGGTTTAATCAGTGCCAAGGAAAAAATTGCGACACACGTTTCATCGTGGTTTAGTGCCAAAGG
CTACCATGAAACCATCAGTTATAGTTTTGTTGATCCTGAGTTGCAGGAAGCGCTTTACCCACAAAAAGAGTTTATGGAAC
TGCTTAATCCAATTTCCTCTGAATTATCGCAAATGCGTGCGGGAATGTGGCCAGGCTTAATTGCATCTATGATTTATAAT
AGCCACAGGCAACAAACCGCAGTGAAATTTTTTGAAATAGGCGTGGTTTTTGATCTGGACGGAGGGCAATTAAAGGAGCG
TTCATGTATTGCTGGCTTGTTAATGGGGGAGCAAGGCAATTTGAATTGGAGTGAATCTGCCCGTTTATTTGATTTTTATG
ATTTAAAAGGGGATTTACAATCCTTGTTTGCTTCATTGAAGCTGGATGATGTTGAATTTATTCAATCATCTCATCATGCG
CTTCACCCCGGCCAGTCCGCTCAGATTGTGATTAACGGCAAGCATTCAGGCTGGATCGGTGTGCTGCATCCTCGCTTGTC
GGATGCCTTTGATCTTGACCAGGATGTGGTTTTGTTTGAATTGAATCTTGAATCATTAATCAATCCGACTATTCCTCTGT
ATAAGCCTATTTCCAAATATCCACAAATAAGGCGAGATTTGTCTTTCCTGGTTGATAGGCAGATAAGTGCCATGCAGATT
GAGCGAGTAATCCGAAATACAGTTAAAGAGGATTGGTTAAAATCGTTTGACGTATTTGACGTGTATATGGGTAAAGGTAT
TCCAGAAGATAAAAAAAGTATTGCTGTGGCTATGACACTACAAGACGATACTCGAACTTTAGTTGATGCCGAGATCAATT
TAACAATTAGTGCTATAATCAAGAAACTGGAAAATGAATTTTCAATCCTTTTGAGGGAATGA

Upstream 100 bases:

>100_bases
AATAGTTATCAGTAATTTTATCTCTATTATCATAAAGAGAGCAAAATTAACGGATGGAAACTTTGAGCCAAATGAACAGT
TACAGAGATGAGGACTTTAA

Downstream 100 bases:

>100_bases
TCGTGAACGCACTAAGCAAAGCAATAATGGCAGAAACATTGTGTGACGAATTAAAATTAAATAAACCAGTTGCTAAAGAA
ATGGTTGAAAATTTTTTTGA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTHVIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCG
AANVRPGLRVALAMIGAHLPGGLQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCGRIIRNLNLEAKTPLWMAERLRR
GGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEINVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGA
NSAVQEQTQHIFLESAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVIESFDKKFL
PGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVIIKETNHFFEVTIPSHRVDLQQDADLVEEIIRLYGYDKLQAQPM
QTSVQAGLISAKEKIATHVSSWFSAKGYHETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYN
SHRQQTAVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFASLKLDDVEFIQSSHHA
LHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELNLESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQI
ERVIRNTVKEDWLKSFDVFDVYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE

Sequences:

>Translated_793_residues
MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTHVIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCG
AANVRPGLRVALAMIGAHLPGGLQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCGRIIRNLNLEAKTPLWMAERLRR
GGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEINVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGA
NSAVQEQTQHIFLESAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVIESFDKKFL
PGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVIIKETNHFFEVTIPSHRVDLQQDADLVEEIIRLYGYDKLQAQPM
QTSVQAGLISAKEKIATHVSSWFSAKGYHETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYN
SHRQQTAVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFASLKLDDVEFIQSSHHA
LHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELNLESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQI
ERVIRNTVKEDWLKSFDVFDVYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE
>Mature_793_residues
MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTHVIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCG
AANVRPGLRVALAMIGAHLPGGLQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCGRIIRNLNLEAKTPLWMAERLRR
GGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEINVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGA
NSAVQEQTQHIFLESAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVIESFDKKFL
PGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVIIKETNHFFEVTIPSHRVDLQQDADLVEEIIRLYGYDKLQAQPM
QTSVQAGLISAKEKIATHVSSWFSAKGYHETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYN
SHRQQTAVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFASLKLDDVEFIQSSHHA
LHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELNLESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQI
ERVIRNTVKEDWLKSFDVFDVYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=797, Percent_Identity=46.4240903387704, Blast_Score=721, Evalue=0.0,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88295; Mature: 88295

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTHVIVAEVLNTKPHPDAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
KLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGGLQIKESKLRGELSQGMLC
CEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCEE
SATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDIDLTPNRADCFSILGVAREVA
CHHHCCCHHCCCCHHEECCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
VLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCGRIIRNLNLEAKTPLWMAERLRR
HHHCCCCCCCCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
GGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEINVRYSASGEQLELLDGQEVVLNDN
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCEEEEECCCEEEEECC
VLVIADKEKPLAMAGIMGGANSAVQEQTQHIFLESAYFNPVTIAGVARKYGLFSDSSQRF
EEEEECCCCCEEEEEECCCCCHHHHHHHHHEEEEECCCCCEEHHHHHHHHCCCCCCHHHH
ERGVDPCLQSKALERATELILSISGGEPGPVIESFDKKFLPGTVSFLFDTTKVKKLTGLS
HHCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCHHCCCCHHHHHHCHHHHHHHCCCC
IPLNEMKNLLEGLGVVIIKETNHFFEVTIPSHRVDLQQDADLVEEIIRLYGYDKLQAQPM
CCHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHCCCCH
QTSVQAGLISAKEKIATHVSSWFSAKGYHETISYSFVDPELQEALYPQKEFMELLNPISS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHHHCCHHHHHHHHHHHHH
ELSQMRAGMWPGLIASMIYNSHRQQTAVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHHEECCCCC
LNWSESARLFDFYDLKGDLQSLFASLKLDDVEFIQSSHHALHPGQSAQIVINGKHSGWIG
CCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCEEE
VLHPRLSDAFDLDQDVVLFELNLESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQI
EECCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
ERVIRNTVKEDWLKSFDVFDVYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAII
HHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEEEECEEHHHHH
KKLENEFSILLRE
HHHHHHHHHEECC
>Mature Secondary Structure
MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTHVIVAEVLNTKPHPDAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
KLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGGLQIKESKLRGELSQGMLC
CEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCEE
SATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDIDLTPNRADCFSILGVAREVA
CHHHCCCHHCCCCHHEECCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
VLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCGRIIRNLNLEAKTPLWMAERLRR
HHHCCCCCCCCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
GGIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEINVRYSASGEQLELLDGQEVVLNDN
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCEEEEECCCEEEEECC
VLVIADKEKPLAMAGIMGGANSAVQEQTQHIFLESAYFNPVTIAGVARKYGLFSDSSQRF
EEEEECCCCCEEEEEECCCCCHHHHHHHHHEEEEECCCCCEEHHHHHHHHCCCCCCHHHH
ERGVDPCLQSKALERATELILSISGGEPGPVIESFDKKFLPGTVSFLFDTTKVKKLTGLS
HHCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCHHCCCCHHHHHHCHHHHHHHCCCC
IPLNEMKNLLEGLGVVIIKETNHFFEVTIPSHRVDLQQDADLVEEIIRLYGYDKLQAQPM
CCHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHCCCCH
QTSVQAGLISAKEKIATHVSSWFSAKGYHETISYSFVDPELQEALYPQKEFMELLNPISS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHHHCCHHHHHHHHHHHHH
ELSQMRAGMWPGLIASMIYNSHRQQTAVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHHEECCCCC
LNWSESARLFDFYDLKGDLQSLFASLKLDDVEFIQSSHHALHPGQSAQIVINGKHSGWIG
CCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCEEE
VLHPRLSDAFDLDQDVVLFELNLESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQI
EECCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
ERVIRNTVKEDWLKSFDVFDVYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAII
HHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEEEECEEHHHHH
KKLENEFSILLRE
HHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA