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The map label for this gene is miaA [H]

Identifier: 54295537

GI number: 54295537

Start: 2995309

End: 2996250

Strand: Reverse

Name: miaA [H]

Synonym: lpl2624

Alternate gene names: 54295537

Gene position: 2996250-2995309 (Counterclockwise)

Preceding gene: 54295538

Following gene: 54295536

Centisome position: 89.56

GC content: 40.23

Gene sequence:

>942_bases
ATGAATAAGCTAGTGTTTTGTTTAATGGGACCAACTGCTTCAGGAAAGACAGGGTTAGCGTGTGAGTTATTAACCCATTT
CCCTTTTGAAATTATTAGTGTTGATTCCGCAATGATTTATAGAGATATGAATATTGGGACAGCGAAACCTTCAATCCACG
AATTACAGAGTGCGCCTCATTATTTAATTGATATTAAAGATCCTGTTGAATCCTATTCGGCAGCTCAGTTTTGTACAGAC
GCTTTGTCTTTGTGTGCTGCAATCATCAAAAGAGGGAATATTCCTTTGTTAGTGGGTGGAACTATGATGTATTTTAATGC
TTTGCAAAAAGGGTTAGCCACTTTGCCTGAAGCCGATGAAGCTGTTCGTAAACGACTGGAGGAGGAAGCATTATCACAGG
GTTGGGATTTTCTTTATCAAAAGCTAAGTCAATTAGATCCGGTAACAGCCGCTCGCATTCATGCTCATGATACGCAAAGA
ATTCAAAGAGCGCTGGAAGTTTATTACTTAACCGGTTCAACGTTATCAACCTATTTGACCGGCCCCCATGAACAGCCTGA
TTATTGTTTTGTTAATCTTGCTCTTTTTCCTGAGCAAAGATCCTGGTTGCATGAACGTATCGCGCAACGGTTTGACGCGA
TGCTTAGTGAGGGATTCATAGAAGAAGTTCAACAATTGCAAGCAAAATGGCCTATTCAAATCAACTTACCCTCCATGCGA
TGTGTTGGATATAGGCAAATACTCGAGTATCTTGCAGGCCATTATGATTATGAGACCATGAGGGAAAAAGGTATAGCAGC
AACAAGGCAGCTGGCCAAACGGCAATTAACCTGGTTGAGGCATTGGGAGGGCGCTTTATTTTATGATTCGCAAAATGTGG
GTTTTAATACGGATATTATAGCGAAAATAAGGGAAATATTAGATAATACTGTCTCTAATTGA

Upstream 100 bases:

>100_bases
CGTATTGAAATGAATCAAGTGTTACTGGAACTCTCTCTTAAAGAAGAGAAACATAATATATGTAAAGCGTTAACTATAGA
TGATTGTCGGATGTTATTGC

Downstream 100 bases:

>100_bases
CATCATGCCGCAAAGGAACAACATGAGGAAAACAACAAAAGAACCTGCAAGTGCTAAAAAAAATTATGTCGTACATAGAC
ATCAATTACCATTGAGTTGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQSAPHYLIDIKDPVESYSAAQFCTD
ALSLCAAIIKRGNIPLLVGGTMMYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
IQRALEVYYLTGSTLSTYLTGPHEQPDYCFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPIQINLPSMR
CVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN

Sequences:

>Translated_313_residues
MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQSAPHYLIDIKDPVESYSAAQFCTD
ALSLCAAIIKRGNIPLLVGGTMMYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
IQRALEVYYLTGSTLSTYLTGPHEQPDYCFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPIQINLPSMR
CVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN
>Mature_313_residues
MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQSAPHYLIDIKDPVESYSAAQFCTD
ALSLCAAIIKRGNIPLLVGGTMMYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
IQRALEVYYLTGSTLSTYLTGPHEQPDYCFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPIQINLPSMR
CVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=29.3929712460064, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI1790613, Length=292, Percent_Identity=53.0821917808219, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25144712, Length=272, Percent_Identity=25, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=32.996632996633, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24649830, Length=328, Percent_Identity=27.4390243902439, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35587; Mature: 35587

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQSAPH
CCCEEEEEECCCCCCCCCHHHHHHHHCCHHEEECCHHHEEEECCCCCCCCHHHHHHCCCC
YLIDIKDPVESYSAAQFCTDALSLCAAIIKRGNIPLLVGGTMMYFNALQKGLATLPEADE
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCHHH
AVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQRIQRALEVYYLTGSTLSTYLT
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCC
GPHEQPDYCFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPIQINLPSMR
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHH
CVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDII
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHH
AKIREILDNTVSN
HHHHHHHHHHCCC
>Mature Secondary Structure
MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQSAPH
CCCEEEEEECCCCCCCCCHHHHHHHHCCHHEEECCHHHEEEECCCCCCCCHHHHHHCCCC
YLIDIKDPVESYSAAQFCTDALSLCAAIIKRGNIPLLVGGTMMYFNALQKGLATLPEADE
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCHHH
AVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQRIQRALEVYYLTGSTLSTYLT
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCC
GPHEQPDYCFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPIQINLPSMR
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHH
CVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDII
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHH
AKIREILDNTVSN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA