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The map label for this gene is rpoH [H]

Identifier: 54295507

GI number: 54295507

Start: 2961053

End: 2961907

Strand: Reverse

Name: rpoH [H]

Synonym: lpl2594

Alternate gene names: 54295507

Gene position: 2961907-2961053 (Counterclockwise)

Preceding gene: 54295508

Following gene: 54295505

Centisome position: 88.53

GC content: 41.64

Gene sequence:

>855_bases
ATGAGTCAACAGTTGCAACTTGCTGCAATGAGTCTACCTGTTGGTAGTCTTGATTCTTATATCCATCGAGTAAATCAAAT
TCCAATGCTGACTTTGGAAGAGGAAATTGCATATGCCGAGCGGTTTCATTCTGAAGGAGATATAGAAGCTGCTCGACAAT
TAGTTCTTGCGCATTTGCGCTATGTAGTTCGTGTAGCCCGCGGTTATCTGGGGTATGGATTGCCTTTAAGTGATTTAATT
CAGGAAGGTAATGTAGGCTTGATGAAAGCCGTAAAACGTTTTGACCCCAAGATGGGCGTACGCCTTGTTTCTTTTGCTGT
TCATTGGATTAAAGCAGAAATTCATGAGTTTGTGCTGCGTAACTGGCGAATAGTTAAAGTTGCTACAACCAAGGCCCAAC
GTAAATTATTTTTTAATTTGCGCCAAATGAAAAATCGATTGGGTTGGTTCAGCAATGAAGAAGTTGATGCTGTCGCCAAA
GATTTGGGCGTTAGCCGAGAAGACGTGTTGTTGATGGAGCAACGTTTAAATGTTATGGATTCATCTTATGATGCGCCAGA
TGTTGATGATAATGATGATGCTTACAAAGCGCCTGAGCGTTATTTGTTTAATGTGAACGATGATCCTGCTGTCTTGTTAG
AGAATGAGGATACTGGCGATCAGGGGCGTGAAAAATTACTGTTTGCTATGGAACAACTGGATGAACGTAGCCAGGATATT
TTACAACAACGCTGGCTTGCCGAAGAAAAACTCACGTTACATGATTTGGCAGAAAAATATGGTGTCTCCGCTGAACGGGT
TAGGCAGCTTGAAAAAAATGCCATGAAGAAAATCCGTCAATACATGGAAGCTTGA

Upstream 100 bases:

>100_bases
TTTGAAAATTTATAGCTTAGCATTCATTTTGGTGAATCCTATCAGTTAATAGGATATAAATTTTAATACTATTTTAGTGT
TAATGACCGGAGGAAGTAGT

Downstream 100 bases:

>100_bases
TCGCATAATTTTTATGCAAATTCCTCTGCATGTGAGGTTCTTTATTCAGCAGACACAATAGTCTTATTGTAGGCTTACTG
ACTTGGATTAGGTTAAATAG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSQQLQLAAMSLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLI
QEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMEA

Sequences:

>Translated_284_residues
MSQQLQLAAMSLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLI
QEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMEA
>Mature_283_residues
SQQLQLAAMSLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQ
EGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAKD
LGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDIL
QQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=59.6491228070175, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=28.996282527881, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789448, Length=244, Percent_Identity=27.0491803278689, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32844; Mature: 32713

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQLQLAAMSLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLR
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLR
HHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAKDLGVSREDVLLMEQRLNVMD
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
CCCCCCCCCCCCCHHCCCHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMEA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQQLQLAAMSLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLR
CCCHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLR
HHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAKDLGVSREDVLLMEQRLNVMD
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
CCCCCCCCCCCCCHHCCCHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMEA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]