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The map label for this gene is gacA/letA [H]

Identifier: 54295484

GI number: 54295484

Start: 2940560

End: 2941219

Strand: Reverse

Name: gacA/letA [H]

Synonym: lpl2571

Alternate gene names: 54295484

Gene position: 2941219-2940560 (Counterclockwise)

Preceding gene: 54295485

Following gene: 54295483

Centisome position: 87.91

GC content: 40.0

Gene sequence:

>660_bases
TTGATTAAAGTATTAATTGTTGATGACCATGCATTGGTTAGAATGGGCATTCGACGATTGCTTGAAGATATGTCGGATGT
TGATGTGGTTGCAGATGCTGAAAGTGGTGAGCAGGCTTTGGCCTATGTGAAAACCCACTCCCCTGATGTCGTATTATTAG
ACATGAAAATGCCTGGGATAGATGGGTGGGAAGTGACACGTCGCTTAAAAAAAACAAACCCAAATATTAAAGTCATAGCA
GTTACAGCTATTTGCTCAGATCCTCTCCCTACTCGAGTATTGCAATTAGGTGCTATGGGATACCTTACTAAAGAGTCCGG
GGCTGAAGAAATGGCTGCCGCAATTCGTAAAGTGGCCAAAGGTGAGAAATATCTTAGTGCCGAGATAGCACAAAAAATGG
CAATTAATAGTCTCCAGGAGTCTCAGGATTCCCCCTTTGATTTATTGTCTGAAAGAGAAATGCAAGTCATGTTGATGATT
ACTAGCGGCATGAATGTACAAGACATTGCTGATAGATTATTTTTAAGTAGTAAAACCATCAATGGCTATCGATATAGGAT
GTTTGAAAAATTGGGGATTAAAAATGATGTGGAGCTTACTTATTTGGCTATGAAGCACCGCATTATTGAGCACCCCAATG
ATTTGTCACAAGATGATTAG

Upstream 100 bases:

>100_bases
ACTTGTGGATTAGAATGACTATGTATTGTAATGATTATCGAGTCTTGTTAACATCGACCAACACAGTTTGTTCATTGACT
GATTGGTCAAGGAGCATTAT

Downstream 100 bases:

>100_bases
ATGTTAATTTCATGAATGACTTACAGCTTTCCGCTGAACTCGCTTTATTTTTAACGAAACTCCCAAGTGAACCGGGTATA
TACCGCATGTTAGACGAAGA

Product: transmission activator LetA

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIA
VTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMI
TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD

Sequences:

>Translated_219_residues
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIA
VTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMI
TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD
>Mature_219_residues
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIA
VTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMI
TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=51.207729468599, Blast_Score=230, Evalue=5e-62,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=38.5, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=27.1844660194175, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=32.0388349514563, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=31.8627450980392, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788546, Length=209, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787473, Length=179, Percent_Identity=27.9329608938547, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787375, Length=128, Percent_Identity=28.90625, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082052, Length=120, Percent_Identity=32.5, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24557; Mature: 24557

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGI
CEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCEEEEEEECCCC
DGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAK
CHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHC
GEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMITSGMNVQDIADRLFLSSKTI
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCC
NGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGI
CEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCEEEEEEECCCC
DGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAK
CHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHC
GEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMITSGMNVQDIADRLFLSSKTI
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCC
NGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]