Click here to switch to the map view.

The map label for this gene is fadE25 [H]

Identifier: 54295071

GI number: 54295071

Start: 2457537

End: 2459228

Strand: Reverse

Name: fadE25 [H]

Synonym: lpl2151

Alternate gene names: 54295071

Gene position: 2459228-2457537 (Counterclockwise)

Preceding gene: 54295072

Following gene: 54295070

Centisome position: 73.5

GC content: 40.43

Gene sequence:

>1692_bases
ATGACCTCTTATGAAAATTTGATACAACTGTCTGAATTTTTTGAGTCATGCCTGGGTAATCCTCTGGAAGACAATTCCCC
TGTTAATTTTCAACAAATTTTGCTCGCTGATGAGCAGGAAAGATTAGCTTCGTCACCAATAGAATTTATTAAACAATGGG
GGTATTTGGACTATACCGTACCTCGTTATTTAGGGGGTAAACTGAGCTCCCTGGATGAGCTATATACTTTAACCAGGTTG
TTGGCCAGGCGTGACATCACGATGGCTATTATGTTTGGATTGGCTTTCTTTGCTGCATTACCAATATGGATAGCTGGAAA
CTCAAAGCAAAAGAAAGATTTGAGTGAGAGCCTTCGCCAGGGAAAGATAGGTGCTTTGGCTTTGACCGAAGAAGAGCATG
GAACCGATTTGACTTCCAATAAGATGAATGCCAGACCAACTGCAGATGGCTGGGAAATTTCGGGACGAAAATGGTGTGTT
AATTTTGCAACCTTGAGTGAGTACGCTGTGGTTTTGTGTCGTACTAATGAAAAAGGCGGGTTGTTAGGTTTTTCTTTGTT
TTATATCGATAAGAAATCAGCCGAAAAGGGTATTTCTCCTACACCCAAGCTGCCGACTCATGGAGTCAGAGGGTTAGATA
TCAGTGGTTTTTCTTTTGATAAAGTGAAAGTATCTAAAGACGCATTAATAGGGAAGGAACAACGTGGTTTGGAAATAACC
TATAAGGTATTCCAGATTTCGCGAACCTTATGCGCCTGTTTGGCTATTGGAGGCGCTGATACTGCTTTGAGGCTGGCATT
ATCATACAGCTTGCAAAGACAGCTGTATGGCAAATCAGTGTTTGAAATACCCGCAGTAAAACAACGGCTTGGCGAATTGT
TTACTCTTTTATTAATCGCGGATTGCACTGCTCAGGTTATGGTTCGCGCCTGTACGGTAATACCGGAAAAAATGAGTCTT
TGGTCAGCGATTATTAAATTCCTTATCCCGCATATTGGAGAAGATATAGCAGAACAGTGTGCCATTATTCTAGGCGCCAG
AGCTTATCTGAGAACCACGCAATGGGCTATTTTTCAGAAAATAAGACGCGACATTCAAGTGGTTGGCTTGTTTGATGGAA
GTACTCAAGTTAATTTATCACTTATTGCTGGTAATTTGTTGCCTCAGGCAGGCATGCGAGGTAAACATCGGGCAGAGCAC
TCCGAAAAAATTGAGAAAATTTTTAATGTTCGTTTGTCTTGCCCTCCTTTTAAGGAGGATAGACTCGGTTTGTTTACTCA
TGAAGAAGATGATGTATTGGCGGGTATCCTGTCGTTAAACTCAATTCCAATCAATCCATTGATCACTACAATACGTCATG
AAATTGAAAAATTGGATCAAGAAGTCATCCGTTTACATGATGAGAAATTATTTGACCCACGCAGTTTAGCCGTTTTTCGT
TTGGCAGAAAAATATTGCTGGATTTTTGCCGCCAGTTGTTGTTTGCAGTTCTGGCACTATAATCAGGAGATACTTTCTGA
TGATTTGCAAAATACAGATTGGCTGGATTTAGCTATGCAGTTAATTCTTAAGCGATTGAATTCAGGTTCGATGATTGATA
GCGTGTTACAAGAAGCTATGAGTGAGCGTTTGTATTCTTTTTATCAGGAAAAACAGCTGTTTTCTATTATGCCAATACAT
ATTGCGGGGTAA

Upstream 100 bases:

>100_bases
CAGCGCAAATTCGCAGTCGCCTTGCCTCTCATTTGGAGTTTTTATATAGAAGAATGGAAACCATGCCCCCGGCAAAACAC
TCTATTGTATAAGGAAAATA

Downstream 100 bases:

>100_bases
TGAGGGATGAATTGGAAAAGATTTTTAATTGCCGCCATGACAAAGAAAAAATACCAGGTTTTTCTGAATGATACCAAGCA
CCCTAAAGAGGCGAGAGAAC

Product: hypothetical protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQERLASSPIEFIKQWGYLDYTVPRYLGGKLSSLDELYTLTRL
LARRDITMAIMFGLAFFAALPIWIAGNSKQKKDLSESLRQGKIGALALTEEEHGTDLTSNKMNARPTADGWEISGRKWCV
NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKSAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
YKVFQISRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPEKMSL
WSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHRAEH
SEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDEKLFDPRSLAVFR
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIH
IAG

Sequences:

>Translated_563_residues
MTSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQERLASSPIEFIKQWGYLDYTVPRYLGGKLSSLDELYTLTRL
LARRDITMAIMFGLAFFAALPIWIAGNSKQKKDLSESLRQGKIGALALTEEEHGTDLTSNKMNARPTADGWEISGRKWCV
NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKSAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
YKVFQISRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPEKMSL
WSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHRAEH
SEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDEKLFDPRSLAVFR
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIH
IAG
>Mature_562_residues
TSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQERLASSPIEFIKQWGYLDYTVPRYLGGKLSSLDELYTLTRLL
ARRDITMAIMFGLAFFAALPIWIAGNSKQKKDLSESLRQGKIGALALTEEEHGTDLTSNKMNARPTADGWEISGRKWCVN
FATLSEYAVVLCRTNEKGGLLGFSLFYIDKKSAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEITY
KVFQISRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPEKMSLW
SAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHRAEHS
EKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDEKLFDPRSLAVFRL
AEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIHI
AG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501857, Length=285, Percent_Identity=27.3684210526316, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI4557233, Length=358, Percent_Identity=23.7430167597765, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI187960098, Length=361, Percent_Identity=24.3767313019391, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4557231, Length=361, Percent_Identity=24.3767313019391, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI76496475, Length=380, Percent_Identity=19.2105263157895, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4557235, Length=380, Percent_Identity=19.2105263157895, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI4501859, Length=357, Percent_Identity=21.5686274509804, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI226958414, Length=297, Percent_Identity=23.9057239057239, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI226958412, Length=276, Percent_Identity=23.9130434782609, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508101, Length=352, Percent_Identity=21.0227272727273, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17570075, Length=376, Percent_Identity=22.6063829787234, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71985184, Length=405, Percent_Identity=20.9876543209877, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538396, Length=335, Percent_Identity=22.089552238806, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534899, Length=369, Percent_Identity=23.3062330623306, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17569725, Length=363, Percent_Identity=22.038567493113, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71982178, Length=223, Percent_Identity=23.7668161434978, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17506239, Length=353, Percent_Identity=19.8300283286119, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71990804, Length=260, Percent_Identity=21.1538461538462, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21356377, Length=357, Percent_Identity=26.0504201680672, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24660351, Length=373, Percent_Identity=24.9329758713137, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24646207, Length=360, Percent_Identity=23.0555555555556, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24666513, Length=282, Percent_Identity=21.6312056737589, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355753, Length=284, Percent_Identity=22.5352112676056, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 63496; Mature: 63365

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQERLASSPIEFIKQWGYLDYTV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHCCHHHHHHHCCCCCEEC
PRYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFAALPIWIAGNSKQKKDLSESLRQ
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHC
GKIGALALTEEEHGTDLTSNKMNARPTADGWEISGRKWCVNFATLSEYAVVLCRTNEKGG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEHHHHCCEEEEEEEECCCCC
LLGFSLFYIDKKSAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
EEEEEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCCCCCCCEEH
YKVFQISRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DCTAQVMVRACTVIPEKMSLWSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHRAEHSEKIEKIFNVRLSCPPFKED
HHCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
RLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDEKLFDPRSLAVFR
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SERLYSFYQEKQLFSIMPIHIAG
HHHHHHHHHHHHHHEECCEEECC
>Mature Secondary Structure 
TSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQERLASSPIEFIKQWGYLDYTV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHCCHHHHHHHCCCCCEEC
PRYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFAALPIWIAGNSKQKKDLSESLRQ
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHC
GKIGALALTEEEHGTDLTSNKMNARPTADGWEISGRKWCVNFATLSEYAVVLCRTNEKGG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEHHHHCCEEEEEEEECCCCC
LLGFSLFYIDKKSAEKGISPTPKLPTHGVRGLDISGFSFDKVKVSKDALIGKEQRGLEIT
EEEEEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCCCCCCCEEH
YKVFQISRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DCTAQVMVRACTVIPEKMSLWSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKHRAEHSEKIEKIFNVRLSCPPFKED
HHCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
RLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDEKLFDPRSLAVFR
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SERLYSFYQEKQLFSIMPIHIAG
HHHHHHHHHHHHHHEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]