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The map label for this gene is gadA [C]

Identifier: 54295022

GI number: 54295022

Start: 2381661

End: 2383478

Strand: Reverse

Name: gadA [C]

Synonym: lpl2102

Alternate gene names: 54295022

Gene position: 2383478-2381661 (Counterclockwise)

Preceding gene: 54295032

Following gene: 54295018

Centisome position: 71.24

GC content: 41.03

Gene sequence:

>1818_bases
ATGTTCGGTTTTATTTCAGATTTATTAACAGCAGCAGTCAGTTCTTTAGATGAATTATTACAAGATACTCCGGCACATCA
AATCATATTAGGCACAGCTGCTCTTTATTTTCTTTATAATCAATACCATAACCCATCGATTTCCAGGTGGTACCTTAGCC
GCAATAACGCCAGTATGAAGCAGCGTATTATTGATAGTGCTTACGCCTTGGCAAAAAATTTACCTGGCGTGAATCAGATC
ATTGAGAAAGAGCTCAATAAAGAACTCTCTTCAACGAGAGAAAAATTGAGAATTCAGCGTTCCGGTATGACTCTCAGGGA
GGAAATACCTGAAGAAGGGCTTTCTCCCCAAGATATTTTGAGTGCATTTGATGTGGATGTGGAAAAATGTCATTTTGATT
TTTTATCCGTAAATAATGACAGCCCTGAGAGGGAGTTTCTTGTTGGAGGAGGGGATGGTAAAGATTCAGGTGCATTATAC
GCTATTCACCCTAAGGAATTGACAGAGCTGCTTAAAGAAGTTTATGGGGCAACTGCTCTAACTAATCCTTTGCATGATAA
ATGGCCTCGAATTAACGCAATGCAAGCGGAGGTGATTCGCTGGTGTCAAAATCTGTTTCATGGTTCAAAAGAAGGTTATG
GTTTATTAACTCATGGGGGTACAACCAGCATTATTGAGGCGATGGCTGCTTATGTCATTCGCGCAAGGGCAAAAGGCATT
GATTATCCTGAAATTGTCGTTCCTGAAACAGCACATGCTGCATTTAAAAAAGCAGCCGAATTAACTGGAGCAATATTAAT
TACGGTTCCTGTTGATAAAAAAACAGGTGCGGTGAATCCCAGGGTCATGAGTTCCTATATTACCAGAAATACTGCCGTAA
TAGTTGGATCTGCCCCATCTTTCATGAATGGAATTCATGATCCTGTCTCTGAGTTAGGTCAATTAGCAAAGAAAAAAAAT
GTTCCTTTTCATGTAGATGCCTGTTTGGGTGGGTTTTTAACGGCTTTTTTAGACACTTCATCCGAGCCTATGGATTTTAG
AGTACCTGGAGTGACTTCCATTTCAGCCGACTTGCATAAATATGGCTGTTGTCCAAAAGGCACCTCTGTTTGTCTTTTTA
GTGAGGATTCGCCTGCTTTGTCAGTTTATGCCGCATTAAACTGGTCAGGTGGATTGTATGCTACGCCAGGAATTCTGGAT
GGTTCAACCAGTGGAGCTCGTGTCGCGGAAGTCTACGCTACATTGTCTTACTATGGGAAAAATAAATATCAGGAAATCGC
CAAAAGCATTATCAAATTACGCAATGCCATTCAAAAAGAATTGACGGCATTAGTGGAGGAAGGCAATGGATTAACCAGCG
AAGATATTTATGTTTATGGTAATCCTCAATGGTCCATTCTGGGGTTTAGAAGTAACACTTGCAATGCCCATTTCATTGCA
GATGAATTGGAGAAGCGGGGATGGAAGCTTAACTTGCTACAAAATCCGGATGGGTTTCATTTATGTTTGACCCATGTGCA
CACTTTAGTAAGGGGCTTCGAAACGCAATTTATTAAAGATTTAAGAGAGGCGGTGATTGATGTTAAAAATTATCCCCCTG
GAAAGAAAGCAAGCGGTAATGTCAAAGTGTATGGTGCTGTCGGTATGATGCCGATTGAACTTCAGAAAGAGATTTGTAAA
CAATATCAAAAAGCCCGCCTTGATTTTACAGCAGCCAGCCACGGGAGCTTGGGGATATTTACAACTTCACCTACAGAAGA
GGACGATGGACTTAGAAACAGGAAAGTTGGGGAACAAAAGGTGCAAACCTCGCTTTGA

Upstream 100 bases:

>100_bases
ATACCACAGAATTTTTGTTATACTGGCGTGCATCAACTGGTCTTTATTTTTTTATGCAAAGTGACTATTATCCAACATTT
TGTTCGTAAGGATATGTGCA

Downstream 100 bases:

>100_bases
ACCAATAGAGCTCTTGCATAACCAGTCTTTATCTTTTTAATGCTGCCTTGCAAACGTTTCTGCGGCACTGATTTACTGCA
TCTAAACTATGCTCGCTGAG

Product: hypothetical protein

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYLSRNNASMKQRIIDSAYALAKNLPGVNQI
IEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVNNDSPEREFLVGGGDGKDSGALY
AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPRVMSSYITRNTAVIVGSAPSFMNGIHDPVSELGQLAKKKN
VPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILD
GSTSGARVAEVYATLSYYGKNKYQEIAKSIIKLRNAIQKELTALVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
DELEKRGWKLNLLQNPDGFHLCLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKASGNVKVYGAVGMMPIELQKEICK
QYQKARLDFTAASHGSLGIFTTSPTEEDDGLRNRKVGEQKVQTSL

Sequences:

>Translated_605_residues
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYLSRNNASMKQRIIDSAYALAKNLPGVNQI
IEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVNNDSPEREFLVGGGDGKDSGALY
AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPRVMSSYITRNTAVIVGSAPSFMNGIHDPVSELGQLAKKKN
VPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILD
GSTSGARVAEVYATLSYYGKNKYQEIAKSIIKLRNAIQKELTALVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
DELEKRGWKLNLLQNPDGFHLCLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKASGNVKVYGAVGMMPIELQKEICK
QYQKARLDFTAASHGSLGIFTTSPTEEDDGLRNRKVGEQKVQTSL
>Mature_605_residues
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYLSRNNASMKQRIIDSAYALAKNLPGVNQI
IEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVNNDSPEREFLVGGGDGKDSGALY
AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPRVMSSYITRNTAVIVGSAPSFMNGIHDPVSELGQLAKKKN
VPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILD
GSTSGARVAEVYATLSYYGKNKYQEIAKSIIKLRNAIQKELTALVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
DELEKRGWKLNLLQNPDGFHLCLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKASGNVKVYGAVGMMPIELQKEICK
QYQKARLDFTAASHGSLGIFTTSPTEEDDGLRNRKVGEQKVQTSL

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=495, Percent_Identity=36.969696969697, Blast_Score=300, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17543922, Length=510, Percent_Identity=34.9019607843137, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17557272, Length=540, Percent_Identity=31.4814814814815, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI25148342, Length=509, Percent_Identity=27.7013752455796, Blast_Score=204, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6320500, Length=487, Percent_Identity=32.2381930184805, Blast_Score=229, Evalue=8e-61,
Organism=Drosophila melanogaster, GI21355963, Length=490, Percent_Identity=36.734693877551, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24654344, Length=490, Percent_Identity=36.734693877551, Blast_Score=306, Evalue=3e-83,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 66495; Mature: 66495

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYLSRNNASMK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
QRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDIL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
SAFDVDVEKCHFDFLSVNNDSPEREFLVGGGDGKDSGALYAIHPKELTELLKEVYGATAL
HHHCCCHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCHHH
TNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
CCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPRVMSSYITRNTAVIVGSAPS
CCCCEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCH
FMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHK
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHH
YGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILDGSTSGARVAEVYATLSYYGK
HCCCCCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCC
NKYQEIAKSIIKLRNAIQKELTALVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHH
DELEKRGWKLNLLQNPDGFHLCLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKASGN
HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VKVYGAVGMMPIELQKEICKQYQKARLDFTAASHGSLGIFTTSPTEEDDGLRNRKVGEQK
EEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCHHH
VQTSL
HHHCC
>Mature Secondary Structure
MFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYLSRNNASMK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
QRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDIL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
SAFDVDVEKCHFDFLSVNNDSPEREFLVGGGDGKDSGALYAIHPKELTELLKEVYGATAL
HHHCCCHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHHCCHHH
TNPLHDKWPRINAMQAEVIRWCQNLFHGSKEGYGLLTHGGTTSIIEAMAAYVIRARAKGI
CCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
DYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPRVMSSYITRNTAVIVGSAPS
CCCCEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCH
FMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHK
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHH
YGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILDGSTSGARVAEVYATLSYYGK
HCCCCCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCC
NKYQEIAKSIIKLRNAIQKELTALVEEGNGLTSEDIYVYGNPQWSILGFRSNTCNAHFIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHH
DELEKRGWKLNLLQNPDGFHLCLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKASGN
HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VKVYGAVGMMPIELQKEICKQYQKARLDFTAASHGSLGIFTTSPTEEDDGLRNRKVGEQK
EEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCHHH
VQTSL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA