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The map label for this gene is grpE [H]

Identifier: 54294924

GI number: 54294924

Start: 2245656

End: 2246255

Strand: Reverse

Name: grpE [H]

Synonym: lpl2003

Alternate gene names: 54294924

Gene position: 2246255-2245656 (Counterclockwise)

Preceding gene: 54294926

Following gene: 54294923

Centisome position: 67.14

GC content: 41.0

Gene sequence:

>600_bases
ATGAGTAAACAAAATAAAAAAGATTGGAAAAAATTTAGAGACGAACACAAAGAAGAACACAAGGTAGAGAATGAGATTCT
GGAAGAAGAAACCGATGAAGAATCACAACATCAAGAACCTGCTTTAGGACATCCCAGCTATACTGCCTTAGAAGAGCAAC
TGACATTGGCGGAACAAAAAGCACATGAAAATTGGGAAAAGTCTGTACGCGCCCTTGCTGAATTGGAGAACGTTCGTCGT
CGTATGGAACGAGAAGTTGCCAATGCTCATAAATATGGCGTAGAAAAACTGATTTCAGCTTTATTGCCTGTAGTGGACAG
TCTTGAGCAAGCATTACAATTAGCGGATAAGAACAGTGATCCATCTATGCATGAAGGGCTTGAGTTAACTATGAAACTCT
TTCTTGACGCATTACAGAAGTTTGATGTGGAACAAATTGATCCTCTTGGACAGACATTTGACCCTCAACAGCATGAGGCA
ATGTCAATGCAACCTGCCCCAGGAGCACCACCGAATTCTGTAATTACTGTATTCCAAAAAGGTTACAAATTGAGCGATCG
AGTAATTCGGCCAGCTCGGGTGATAGTTTCCACCAAATAA

Upstream 100 bases:

>100_bases
GACCTGAATTTTAAACCAAGATTTAAAATCCTAACAAGGGCTTGAAAATTGCAAATACAATCCCCATTTGTAGGAATCGA
GTTAGTCAGGAGCGATTTTG

Downstream 100 bases:

>100_bases
TCTGGTGTTTCAGTTGAGGTATTTTTAAACAAATTTTTAGATGCAGTGTATTCAGCTTTTTTTAGGGTGATAGCTTGAAA
TCGTCATGATGCACCCCATA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSKQNKKDWKKFRDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQKAHENWEKSVRALAELENVRR
RMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEA
MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK

Sequences:

>Translated_199_residues
MSKQNKKDWKKFRDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQKAHENWEKSVRALAELENVRR
RMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEA
MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK
>Mature_198_residues
SKQNKKDWKKFRDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQKAHENWEKSVRALAELENVRRR
MEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEAM
SMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=179, Percent_Identity=36.8715083798883, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI40255109, Length=138, Percent_Identity=34.7826086956522, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1788967, Length=186, Percent_Identity=40.3225806451613, Blast_Score=121, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17552458, Length=143, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=193, Percent_Identity=32.1243523316062, Blast_Score=96, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=34.8571428571429, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22832; Mature: 22701

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQNKKDWKKFRDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEAMSMQPAPGAPPNSVITVFQK
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHH
GYKLSDRVIRPARVIVSTK
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQNKKDWKKFRDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEAMSMQPAPGAPPNSVITVFQK
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHH
GYKLSDRVIRPARVIVSTK
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA