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The map label for this gene is queA [H]

Identifier: 54294901

GI number: 54294901

Start: 2220878

End: 2221891

Strand: Reverse

Name: queA [H]

Synonym: lpl1980

Alternate gene names: 54294901

Gene position: 2221891-2220878 (Counterclockwise)

Preceding gene: 54294902

Following gene: 54294900

Centisome position: 66.41

GC content: 40.63

Gene sequence:

>1014_bases
ATGAATAAGCAAGATTTTTATTTTGATTTGCCTTCCGAATTAATAGCCCAATATCCTCTGGCGAATAGAAGTGATTCACG
GCTTTTAATTTATAATCGACAGACAGAAGAATATGGCCATTATCAATTCCGGGAAATTGCAGATTTCTTACAGCCAGGGG
ACTTATTGGTAATGAATGACAGCAAAGTGATACCAGCACGCTTATACGGCCACAAAGCTACTGGCGGCAAGGTTGAACTG
TTAGTAGAGAGGATAACTGGCGATTTTACTTTCCTTGCCCATATCAAAGCGAGCAAATCATTAAAATCAAACGATCTCAT
TTATCTTGATGCCGGTAAGCGGCTTGAAGTATTACAAAGGCAGGATGATTTATTTCTTTGTAAGGCGAGCGAAAATATTT
TAGATCTTTTAAATGATCTCGGACATATTCCTTTACCACCTTATATCGACAGAGAAGATGAATCACTGGATAAGGAGAGA
TATCAAACCGTCTATGCCAAGTGCGCGGGGTCTGTTGCTGCGCCTACAGCAGGATTGCATTTTGATGAAGCTGTTTTATC
CAGTATTAGAGCGCGAGGAGTTAATATTGCCTATATCACTCTCCATGTAGGGGCAGGAACATTCAGGCCAGTTCGCTGTG
AACGCATTCAGGATCATAAAATGCATAGTGAGTGGTTTACGGTTAGTCCAGATCTGTGCACTGCAGTGAAGGCTGCCAAA
TCTATGGGAAATAGAGTAATTGCTGTTGGGACTACTGCATTGCGCAGCCTTGAAAGCGCTGCTATGGGAGGAGAGCTAAT
TCCATGTAGTCGTGATACCGATATTTTCATTTATCCTGGCTACCAGTTTAAAGTTTGTGATGGATTAATAACTAATTTTC
ATTTGCCTGAATCAACTCTGGTGATGTTGGTGTCTGCTTTTATTGGGCACCAGGAGTGTATGGCTCTTTATCAAGAAGCA
ATCGATAAAAGGTATCGTTTTTTTAGTTATGGAGATGCCAGTTTATTGTTATAA

Upstream 100 bases:

>100_bases
AATGATATTAATTTTCTAATAAAATGAATAAATTCCTGATAATTGATTTTAAGACTAAAGTTCGGTGTATAATTGCACCA
ATTTGTTTTTAGGTTGTTCT

Downstream 100 bases:

>100_bases
AGTGCTCTGGAATATAATAATTTTGGCTACAGTTAGAGGATAGTGGATGTTGAGTACAAATCAAAATTTTGTTCCTGTTT
TAAATACTGAAGCAGGACTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVEL
LVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQRQDDLFLCKASENILDLLNDLGHIPLPPYIDREDESLDKER
YQTVYAKCAGSVAAPTAGLHFDEAVLSSIRARGVNIAYITLHVGAGTFRPVRCERIQDHKMHSEWFTVSPDLCTAVKAAK
SMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEA
IDKRYRFFSYGDASLLL

Sequences:

>Translated_337_residues
MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVEL
LVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQRQDDLFLCKASENILDLLNDLGHIPLPPYIDREDESLDKER
YQTVYAKCAGSVAAPTAGLHFDEAVLSSIRARGVNIAYITLHVGAGTFRPVRCERIQDHKMHSEWFTVSPDLCTAVKAAK
SMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEA
IDKRYRFFSYGDASLLL
>Mature_337_residues
MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVEL
LVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQRQDDLFLCKASENILDLLNDLGHIPLPPYIDREDESLDKER
YQTVYAKCAGSVAAPTAGLHFDEAVLSSIRARGVNIAYITLHVGAGTFRPVRCERIQDHKMHSEWFTVSPDLCTAVKAAK
SMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEA
IDKRYRFFSYGDASLLL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=343, Percent_Identity=54.8104956268222, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37892; Mature: 37892

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMND
CCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECC
SKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQR
CCCCCHHEECCCCCCCHHEEHEEHHCCCEEEEEEEHHCCCCCCCCEEEEECCCHHHHHHC
QDDLFLCKASENILDLLNDLGHIPLPPYIDREDESLDKERYQTVYAKCAGSVAAPTAGLH
CCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FDEAVLSSIRARGVNIAYITLHVGAGTFRPVRCERIQDHKMHSEWFTVSPDLCTAVKAAK
HHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
SMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTL
HHCCEEEEECHHHHHHHHHHHCCCEEECCCCCCCEEEECCCEEEECCHHHHCCCCCHHHH
VMLVSAFIGHQECMALYQEAIDKRYRFFSYGDASLLL
HHHHHHHHCHHHHHHHHHHHHHHHHEEEEECCCEECC
>Mature Secondary Structure
MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMND
CCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECC
SKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQR
CCCCCHHEECCCCCCCHHEEHEEHHCCCEEEEEEEHHCCCCCCCCEEEEECCCHHHHHHC
QDDLFLCKASENILDLLNDLGHIPLPPYIDREDESLDKERYQTVYAKCAGSVAAPTAGLH
CCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FDEAVLSSIRARGVNIAYITLHVGAGTFRPVRCERIQDHKMHSEWFTVSPDLCTAVKAAK
HHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
SMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTL
HHCCEEEEECHHHHHHHHHHHCCCEEECCCCCCCEEEECCCEEEECCHHHHCCCCCHHHH
VMLVSAFIGHQECMALYQEAIDKRYRFFSYGDASLLL
HHHHHHHHCHHHHHHHHHHHHHHHHEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA