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The map label for this gene is fliM [H]

Identifier: 54294679

GI number: 54294679

Start: 1958952

End: 1960013

Strand: Reverse

Name: fliM [H]

Synonym: lpl1756

Alternate gene names: 54294679

Gene position: 1960013-1958952 (Counterclockwise)

Preceding gene: 54294680

Following gene: 54294678

Centisome position: 58.58

GC content: 38.7

Gene sequence:

>1062_bases
ATGACTGAGAAAGATGTGTTATCGCAAGAAGAAATTGATGCATTACTGGATTCAGTGGATGAGTCAATTGATGGTGAAAC
GAATGATGAAGAATTGAACTCAAGCGATCAGCCAGCTCGTAAAAAACCAGAAGATACTACCGGCCAGGAATTAAACTCCA
CTTTTGATTCTGATATAAAAGTAGCAGAAGAAGGAGTCAAAACACTTAATTTTACCGGACAAGAGCGTATCGTTAAGGGA
CAACTTCCGGTATTAGACAAAATTTATGACAGGGCAGTCCGTCTGTTTGCAGCAGATATTTATCATTTAACGGCTAGAGA
TTTTGAAATTAAACAAGATCCGCTGCTTATTACAAAGCATAAGGAATTTATGAGGAGTTTACCTAATCCTAGCCTGATAG
GTATCTATAAATTCAAGCCTCTTCGTGGTAAAGGAATTATTTTGTTTGATAGTACCTTTGTATATGACTTGGTCGATTAT
TATTTTGGCGGAAATAGCCAGTTTGGTGCTCAAAAGGATAAAACTGATTTTACTGCTACCGAACTTCGTGTTATGGAGGT
CGTAACGAAAAAACTGGTAACTAATTTAACTCATGCCTGGGAACCAATAATTCAACTGGATGTAACAAAATTCAATGACG
AAACAAATCCCCAATTAGTTAATATTGCCGAGCCAGAAGAAATGTTGCTTGTGGCGCGGTTCCTCTTGAATTTTGGTAAG
GAAACCGGGGCTTTTTACTTTATTTTGCCTTACTCCATGCTTGAACCTATCAAGCAGCAATTGGAGCTAGGGGCATCAAG
ACCTGATGATGAAATTGATCCTAACTGGATTAATTCTCTGAAAGAAGAACTCATGGACGTTGAGTTGACGGTAAGTGCTT
CTATGGCGGAAACAGTCAGTACTCTGGGTCAGGTAATGGCTTGGAAAGTGGGAGATTTTGTCCCTTTGGAAATAAACGAG
GAAGTAACTCTGGATATAGAAGGGACACCAAGCTTTACTGCAACCTTAGGCAGTGCCAATGAAAAACGAGCTTTGAAAAT
CATTAAAATAATACGTTATTAG

Upstream 100 bases:

>100_bases
CTATAGTTGTTACTGATGGATTGAGCCAATTTTAAACTAATCAACCCTTCAATTTTAAAATAACTTTGAAAAACCTGGTT
GAGATGCGAATGGATTGCTT

Downstream 100 bases:

>100_bases
GGGATAAGGATGACTCAAGTGAACGAAAACACTACGGAGCAATTAAATCAAGGGAATCCACTGAATCCAGACCCTGGAAA
TGAGAAACTGGAATTAATTT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTEKDVLSQEEIDALLDSVDESIDGETNDEELNSSDQPARKKPEDTTGQELNSTFDSDIKVAEEGVKTLNFTGQERIVKG
QLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLTHAWEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFLLNFGK
ETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEINE
EVTLDIEGTPSFTATLGSANEKRALKIIKIIRY

Sequences:

>Translated_353_residues
MTEKDVLSQEEIDALLDSVDESIDGETNDEELNSSDQPARKKPEDTTGQELNSTFDSDIKVAEEGVKTLNFTGQERIVKG
QLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLTHAWEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFLLNFGK
ETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEINE
EVTLDIEGTPSFTATLGSANEKRALKIIKIIRY
>Mature_352_residues
TEKDVLSQEEIDALLDSVDESIDGETNDEELNSSDQPARKKPEDTTGQELNSTFDSDIKVAEEGVKTLNFTGQERIVKGQ
LPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDYY
FGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLTHAWEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFLLNFGKE
TGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEINEE
VTLDIEGTPSFTATLGSANEKRALKIIKIIRY

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=350, Percent_Identity=29.1428571428571, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 39919; Mature: 39788

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKDVLSQEEIDALLDSVDESIDGETNDEELNSSDQPARKKPEDTTGQELNSTFDSDIK
CCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHCCHHH
VAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKH
HHHHHHHEECCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECC
KEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDYYFGGNSQFGAQKDKTDFTAT
HHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
ELRVMEVVTKKLVTNLTHAWEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFLLNFGK
HHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCC
ETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVS
CCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEEHHHHHHHHH
TLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTATLGSANEKRALKIIKIIRY
HHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEKDVLSQEEIDALLDSVDESIDGETNDEELNSSDQPARKKPEDTTGQELNSTFDSDIK
CCHHHCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHCCHHH
VAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKH
HHHHHHHEECCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECC
KEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDYYFGGNSQFGAQKDKTDFTAT
HHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
ELRVMEVVTKKLVTNLTHAWEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFLLNFGK
HHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCC
ETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVS
CCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEEHHHHHHHHH
TLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTATLGSANEKRALKIIKIIRY
HHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]