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The map label for this gene is folD [H]

Identifier: 54294196

GI number: 54294196

Start: 1401512

End: 1402366

Strand: Reverse

Name: folD [H]

Synonym: lpl1260

Alternate gene names: 54294196

Gene position: 1402366-1401512 (Counterclockwise)

Preceding gene: 54294197

Following gene: 54294194

Centisome position: 41.92

GC content: 40.35

Gene sequence:

>855_bases
ATGCCTGCATCACTAATTGATGGAAGAGAAATCTCTGCCCTTCGCAGAACTGAACTAAAGCAAAGAGTACAATACCATGT
GGAGCAAGGGCAAAGGGCGCCTGGACTTGCTGTTGTTTTAATTGGCAATGACCCTGCATCAGTGATTTATGTCAGTAACA
AAAGAAAAGCTTGTGAAGAAGTAGGGATCACCTCTCATTCTTACGATTTGCCTGCCGAAACAACTCAGGAAAAATTAATT
GAACTGATCAATGAGTTAAATCAATCGGATAAAATTGATGGTATTTTAATCCAATTACCTTTGCCCAAACATATTAACGA
AAGAACTATAATAGAACACATAAAGCCCGAGAAAGACGTGGATGGATTTCATCCTTATAATTTAGGCCGACTTGCTCAAC
GAAACCCATTTTTAAGACCATGCACCCCCTTGGGGATTATGAATTTATTACATCACTATGAGCTCAATGTAAAAAGAAAA
CATGCCGTAGTGATTGGGGCATCCAATATTGTTGGAAGGCCAATGAGTCTGGAGTTACTATTAGCTGGTGCTACCGTAAC
AATATGCCACAAGTTTACCCAACAATTACAGAAGTTTGTTGAGATTGCTGATTTTCTAATAGTAGCTACTGGAAAGATGG
ACGTCATTGCCACTGACTGGCTCAGAGAGCATCAAGTAGTGATTGATGTAGGAATGCATCGTTTGCCTGATGGCAGTATC
AGGGGTGATATTGATTTTAAGAAGGCAGTCGAAAAAGTAGCCTGGATCACACCTGTTCCAGGGGGAGTTGGCCCAATGAC
TATTGTCACTTTGCTTGAAAATACTATGATGTCAGCAGCAAGATTAAGAGAATGA

Upstream 100 bases:

>100_bases
AACTGCAAGAATAAGTGTATTTTCCTTGTTGCATATGAATCATATTGCATTAGAATAACCCGTAATTCATAATGTACTCC
ATAAAAATTGCGAGAAGAAA

Downstream 100 bases:

>100_bases
AGTTATTTATCCAGTTCATCCCAATCAAATTCACCACTTAGCTCATCAAAATCGTCTTTGAACTCTTCTTTTAGACGCTT
ACGTTCAAGCCTATCTTCAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLI
ELINELNQSDKIDGILIQLPLPKHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE

Sequences:

>Translated_284_residues
MPASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLI
ELINELNQSDKIDGILIQLPLPKHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE
>Mature_283_residues
PASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLIE
LINELNQSDKIDGILIQLPLPKHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRKH
AVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSIR
GDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=40.4761904761905, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=39.6610169491525, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=40.6779661016949, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=24.5, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=57.7060931899642, Blast_Score=341, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17568735, Length=296, Percent_Identity=39.1891891891892, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=40.5594405594406, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6321643, Length=303, Percent_Identity=37.953795379538, Blast_Score=212, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=322, Percent_Identity=25.4658385093168, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=43.1972789115646, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=43.1972789115646, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31699; Mature: 31568

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHH
VGITSHSYDLPAETTQEKLIELINELNQSDKIDGILIQLPLPKHINERTIIEHIKPEKDV
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRKHAVVIGASNIVGRPMSLELL
CCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECHHHHCCCCEEEEE
LAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
EECCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHCEEEEEECCCCCCCCCC
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE
CCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHH
VGITSHSYDLPAETTQEKLIELINELNQSDKIDGILIQLPLPKHINERTIIEHIKPEKDV
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRKHAVVIGASNIVGRPMSLELL
CCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECHHHHCCCCEEEEE
LAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
EECCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHCEEEEEECCCCCCCCCC
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE
CCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA