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The map label for this gene is hisF [H]

Identifier: 54294138

GI number: 54294138

Start: 1344921

End: 1345688

Strand: Reverse

Name: hisF [H]

Synonym: lpl1202

Alternate gene names: 54294138

Gene position: 1345688-1344921 (Counterclockwise)

Preceding gene: 54294139

Following gene: 54294137

Centisome position: 40.22

GC content: 39.84

Gene sequence:

>768_bases
ATGTTAACCAAGCGAATCATTCCCTGTCTTGACGTTCGGGATAATCAGGTTGTCAAGGGAGTAAAATTTCGCAACCATAG
AATAATTGGAGACATACTCCCATTAGCTGCTCACTACTCAGCATCAGGCGCAGACGAATTGGTATTTTATGATATTACAG
CAAGTAGCGATGACCGATCTGTTTGTCCTGTTTGGGTCAATAAAGTGGCGGCTGCGATCAACATTCCATTTACAGTGGCT
GGAGGCATCCGAACCTTGTACCAAGCAAGATCAATTTTAAATGCCGGAGCAGATAAAATCTCAATCAACAGCCCGGCATT
GGAAAACCCTTCATTGATTAACGAATTGAGCAAAGAATTTGGAAAACAATGTGTGGTAATAGGAATTGATAGTCAGTGGA
TAGTTGATGATTATTATGTCTATCAGTATACAGGCAATGAAAATAGAAGTATCAATTCAAAACGCAAAACTCGAGAATGG
ATATATGAAATTCAAGACCGCGGTGCTGGTGAAATAGTCCTTAATTGCATGCAATCTGATGGCGTTCGAATGGGATATGA
TATTGCACAGCTATCAGAAATGATTAACCTCTGCCACGTTCCCCTCATCGCATCGGGCGGTGCTGGAAAAATTGATGATT
TTGTTGAAGTATTTAAAAAATCTCGTGTCAGTGGGGCTTTGGCAGCAAGCGTTTTTCATGACAAAATACTTGCAATCAAT
GACATAAAACTAGCATTACAAACAGAAAATATTGAGGTAAGACTATGA

Upstream 100 bases:

>100_bases
GCCATATGCAGGATATTACAACACTGGGTTCATTAGGAATATCCGCCGTAATCCTTGGGCTAATGCTTTATCAAGATAAT
GTGAATTTCGAGGAGTTATT

Downstream 100 bases:

>100_bases
GTGACTTGGACATTAACTCTCTTGATTGGCAAAAAATGAATGGACTATTGCCGGCAATAATCCAAAACGCAGAAAATGGT
AAAGTATTGATGCTAGGTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSASGADELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVA
GGIRTLYQARSILNAGADKISINSPALENPSLINELSKEFGKQCVVIGIDSQWIVDDYYVYQYTGNENRSINSKRKTREW
IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSRVSGALAASVFHDKILAIN
DIKLALQTENIEVRL

Sequences:

>Translated_255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSASGADELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVA
GGIRTLYQARSILNAGADKISINSPALENPSLINELSKEFGKQCVVIGIDSQWIVDDYYVYQYTGNENRSINSKRKTREW
IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSRVSGALAASVFHDKILAIN
DIKLALQTENIEVRL
>Mature_255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSASGADELVFYDITASSDDRSVCPVWVNKVAAAINIPFTVA
GGIRTLYQARSILNAGADKISINSPALENPSLINELSKEFGKQCVVIGIDSQWIVDDYYVYQYTGNENRSINSKRKTREW
IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSRVSGALAASVFHDKILAIN
DIKLALQTENIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=59.5330739299611, Blast_Score=316, Evalue=1e-87,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=25.1082251082251, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=30.188679245283, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28207; Mature: 28207

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSASGADELVFYDITASSDDRS
CCCCCCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
VCPVWVNKVAAAINIPFTVAGGIRTLYQARSILNAGADKISINSPALENPSLINELSKEF
CCHHHHHHHHHHEECCEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
GKQCVVIGIDSQWIVDDYYVYQYTGNENRSINSKRKTREWIYEIQDRGAGEIVLNCMQSD
CCEEEEEECCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCC
GVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSRVSGALAASVFHDKILAIN
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEEEE
DIKLALQTENIEVRL
EEEEEEEECCEEEEC
>Mature Secondary Structure
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGDILPLAAHYSASGADELVFYDITASSDDRS
CCCCCCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
VCPVWVNKVAAAINIPFTVAGGIRTLYQARSILNAGADKISINSPALENPSLINELSKEF
CCHHHHHHHHHHEECCEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
GKQCVVIGIDSQWIVDDYYVYQYTGNENRSINSKRKTREWIYEIQDRGAGEIVLNCMQSD
CCEEEEEECCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCC
GVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSRVSGALAASVFHDKILAIN
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEEEE
DIKLALQTENIEVRL
EEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA