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The map label for this gene is yjeA [H]

Identifier: 54293590

GI number: 54293590

Start: 692631

End: 693584

Strand: Direct

Name: yjeA [H]

Synonym: lpl0642

Alternate gene names: 54293590

Gene position: 692631-693584 (Clockwise)

Preceding gene: 54293589

Following gene: 54293594

Centisome position: 20.7

GC content: 42.98

Gene sequence:

>954_bases
ATGTCTGATAACTCCTGGTTGCCCTCTGCTTCAATTCAATTATTACGTGCGCGAGCCGAGCTAATAGGTAAAATCCGTCA
ATTTTTTACTGAACGTCAATATCTGGAAGTGGAAACCCCTGTGATGGCGCGGCATGGGGTTACAGATGTTTACCTGAGCA
ATATCAAAGCGATTTTCAGAGGTGAAACATGCTATTTACAAACTTCCCCGGAATACCATATGAAACGGCTGTTAGCTGCT
GGCAGTGGGCCTATTTTTCAATTAGCGCGAGTTTTTCGCGATGACGAGTTAGGGCGTTGGCATAATCCGGAATTTACTTT
ACTTGAATGGTATCAATTAAATATCAATCACCATGTTTTGATGGATGAAGTGGATTTATTATTACAAATTATTCTTAAAT
CCCAGCCTATGATCAGAAAAACCTATCAACAGGCCTTTGAAGAGGCTTGCGGTATTGACCCTTTTTCTACATCCATAGAA
CAATTCACCAAGATATTAGCACAGCATGGTTTAGAAGGTGTCTTATCTCCTGACGAACAAGACAGGGATCAATATCTGTT
TTTATTAATGAGCCATGTCGTGGAGCCTTTTTTGGCAGAAGAATCAACTCCCGTTGCTGTGTATCACTTCCCTGCCTCCC
AGGCTGCCCTGGCACAAATACAAGAGGGCTTGGCTGAGCGTTTTGAGGTGTACTATCAGGGAGTTGAATTGGCTAATGGT
TTTCATGAGTTGACTGATGTCGAAGCGCAAAAAAAGCGTTTTATGCTGGATCAGCAACATAGAAAGCAAAAGGGATTGAC
GGCAATGGAAGCCGATGCCTATCTTTTGGAAGCCCTGGAACATGGGTTACCACCTTGCAGCGGGGTGGCTCTTGGGGTTG
ATCGATTGTTGGCGCTCGCCTTAAAACAACCAAGCATAGAAAAAATTCTCTCATTTGATTTTTCAAGGTCTTAA

Upstream 100 bases:

>100_bases
GGGTCAGCGATTGCACTGTTGAGTTGGTGTGGGAGCCGCCCTGGTCTCAGGACCGCATGACAGAGGCTGCCCGCTTGGAG
TTGGGAATATTTTATTGATC

Downstream 100 bases:

>100_bases
GTCATAGAAAATTAAGTATTAGCTTGTCATTATGATATTTTTGCAGACATAATCACTAAATCCTGGTTATGTTTATACTG
GATAAAAAAACGTGACTTCA

Product: hypothetical protein

Products: AMP; diphosphate; L-lysyl-tRNALys [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMARHGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAA
GSGPIFQLARVFRDDELGRWHNPEFTLLEWYQLNINHHVLMDEVDLLLQIILKSQPMIRKTYQQAFEEACGIDPFSTSIE
QFTKILAQHGLEGVLSPDEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALAQIQEGLAERFEVYYQGVELANG
FHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLLEALEHGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS

Sequences:

>Translated_317_residues
MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMARHGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAA
GSGPIFQLARVFRDDELGRWHNPEFTLLEWYQLNINHHVLMDEVDLLLQIILKSQPMIRKTYQQAFEEACGIDPFSTSIE
QFTKILAQHGLEGVLSPDEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALAQIQEGLAERFEVYYQGVELANG
FHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLLEALEHGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS
>Mature_316_residues
SDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMARHGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAAG
SGPIFQLARVFRDDELGRWHNPEFTLLEWYQLNINHHVLMDEVDLLLQIILKSQPMIRKTYQQAFEEACGIDPFSTSIEQ
FTKILAQHGLEGVLSPDEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALAQIQEGLAERFEVYYQGVELANGF
HELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLLEALEHGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=329, Percent_Identity=26.1398176291793, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI5031815, Length=329, Percent_Identity=26.1398176291793, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=49.6894409937888, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI1789256, Length=327, Percent_Identity=27.82874617737, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790571, Length=336, Percent_Identity=26.4880952380952, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320242, Length=334, Percent_Identity=26.9461077844311, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324256, Length=348, Percent_Identity=23.5632183908046, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640849, Length=332, Percent_Identity=25.3012048192771, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24640851, Length=332, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6 [C]

Molecular weight: Translated: 36216; Mature: 36085

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMARHGVTDVYLSNIKAIFR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCCCHHHHHHHHHHHHC
GETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHNPEFTLLEWYQLNINHHVL
CCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHH
MDEVDLLLQIILKSQPMIRKTYQQAFEEACGIDPFSTSIEQFTKILAQHGLEGVLSPDEQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALAQIQEGLAERFEVYYQGVELANG
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLLEALEHGLPPCSGVALGVDRLLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LKQPSIEKILSFDFSRS
HCCCCHHHHHHCCCCCC
>Mature Secondary Structure 
SDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMARHGVTDVYLSNIKAIFR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCCCHHHHHHHHHHHHC
GETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHNPEFTLLEWYQLNINHHVL
CCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHH
MDEVDLLLQIILKSQPMIRKTYQQAFEEACGIDPFSTSIEQFTKILAQHGLEGVLSPDEQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALAQIQEGLAERFEVYYQGVELANG
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLLEALEHGLPPCSGVALGVDRLLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LKQPSIEKILSFDFSRS
HCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNALys [C]

Specific reaction: ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]