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The map label for this gene is sucB [H]

Identifier: 54293528

GI number: 54293528

Start: 626760

End: 627989

Strand: Direct

Name: sucB [H]

Synonym: lpl0579

Alternate gene names: 54293528

Gene position: 626760-627989 (Clockwise)

Preceding gene: 54293527

Following gene: 54293529

Centisome position: 18.73

GC content: 38.78

Gene sequence:

>1230_bases
ATGTCTATTGAAGTCAAAGTACCTGTTCTACCCGAGTCAGTAGCTGATGCAACAGTGGCTGCGTGGCATAAGAAAGTGGG
TGATAAAGTATCTCGCGATGAGAACCTAGTTGATCTGGAAACAGATAAAGTTGTGCTTGAAGTTCCATCACCTGTGGATG
GGGTATTGTCTGACATTCTGTTTAATACCGGAGATACGGTTGGTTCAGGAGACTTACTGGCAAAAATTTCTCAAAGTGTT
TCTGTAGAATCTCAAAAAACTGAGAAAGAAGAGAAACCTGTCAAAAAAGAAGAAATAAAAATTTCCGAAAGTGAATCAGT
TAGCACAAAAGAAGATAAGTCAACCAGTCCGGTAGTAAGACGGATGATGGCTGAAAATGATTTGAAAGCCGGGCAGATTC
AAGGTACCGGTAAAGATGGCAGGATCACCAAAGAAGATGTTTTATCTTACATAGAATCCAATAGAGAAAAATCAAACCAA
ACTGCTGAAGTTCAAAAAGAATCTTCTATGAAAGCACTTTCTTTTGGTGGCAGGGAAGAAAAACGTGTCCCTATGACGAG
ACTAAGAGCCAAGATTGCGGAACGTTTATTACAAGCACAGCACAATGCTGCCATGTTAACCACTTTTAACGAAGTGAATT
TAAAGGCAGTTATGGATATGCGTTCTCAGTATAAAGATAGCTTTGAAAAAAAACATGGTGTAAAACTTGGATTTATGTCT
TTTTTTACGAAGGCGGTAGTGGAGTCACTTAAACGATTTCCGGCTGTAAATGCTTCAATTGATGGCCAAGATATTGTTTA
TCATGGCTTCTATGATATAGGAATTGCTGTTTCTACTGAAAGAGGTTTAGTCGTTCCGGTGATTAGAGACGCTGATCAGT
TAAGTATGGCAGAAATTGAATTAGCCATTAATGACGCTGCATCAAAAGCCAGAACCGGAAAATTATCTATGGAAGAGATG
CAAGGGGGGACATTTACCATAACTAACGGTGGAGTCTTCGGTTCACTATTAGCTACTCCCATTATCAACCCACCGCAAAC
TGGTATTTTAGGGATGCATAAAATTGAGGAAAGACCTATTGTAGAGAAAGGGCAAATAGTAATCCGTCCTATGATGTATG
TCGCTTTATCCTATGATCATCGTTTAATTGATGGAAAAGATTCTGTACAATTTTTAGTGAGTGTTAAAGAGCTGTTGGAA
GATCCATCTCGACTTTTACTGAATGTTTAA

Upstream 100 bases:

>100_bases
GTCCCGGGTTGCATGCGGAGCAACAAGTTGCTTTGGTTGAGCAAGCATTACTTGGTAAAAAATAAATCTAAACAATATAA
TATTAAGAGAAGGTATAACC

Downstream 100 bases:

>100_bases
TGAGTCATGTTTAACTGCAAGACCCGTCTTGACGGGTTTTGCTTTTTTAAAAGGTAATTAGAATGAATTTACATGAATAT
CAAGCCAAGCAATTGTTCGC

Product: dihydrolipoamide succinyltransferase, E2 subunit

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKVVLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSV
SVESQKTEKEEKPVKKEEIKISESESVSTKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
TAEVQKESSMKALSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVMDMRSQYKDSFEKKHGVKLGFMS
FFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIAVSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEM
QGGTFTITNGGVFGSLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFLVSVKELLE
DPSRLLLNV

Sequences:

>Translated_409_residues
MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKVVLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSV
SVESQKTEKEEKPVKKEEIKISESESVSTKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
TAEVQKESSMKALSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVMDMRSQYKDSFEKKHGVKLGFMS
FFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIAVSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEM
QGGTFTITNGGVFGSLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFLVSVKELLE
DPSRLLLNV
>Mature_408_residues
SIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKVVLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSVS
VESQKTEKEEKPVKKEEIKISESESVSTKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQT
AEVQKESSMKALSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVMDMRSQYKDSFEKKHGVKLGFMSF
FTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIAVSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEMQ
GGTFTITNGGVFGSLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFLVSVKELLED
PSRLLLNV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=236, Percent_Identity=53.8135593220339, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=30.1624129930394, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=29.4930875576037, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=29.7117516629712, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=29.7117516629712, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=38.0952380952381, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1786946, Length=412, Percent_Identity=54.8543689320388, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=29.97542997543, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=407, Percent_Identity=39.5577395577396, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=28.1323877068558, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.4784580498866, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=29.4303797468354, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=43.3823529411765, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=27.3942093541203, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=57.6419213973799, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=28.2973621103118, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=31.0043668122271, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=31.0043668122271, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45026; Mature: 44895

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00867 CPSASE_2 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKVVLEVPSPVDGVLSDIL
CCEEEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCEEEECCCEEEEECCCCHHHHHHHHH
FNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPVKKEEIKISESESVSTKEDKSTSPVVR
HCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCHHHH
RMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQTAEVQKESSMKALSFGGREE
HHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHCHHHHHCCCCCC
KRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVMDMRSQYKDSFEKKHGVKLGFMS
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEHHHH
FFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIAVSTERGLVVPVIRDADQLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHEE
LAINDAASKARTGKLSMEEMQGGTFTITNGGVFGSLLATPIINPPQTGILGMHKIEERPI
EEEHHHHHHHHCCCCCHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCC
VEKGQIVIRPMMYVALSYDHRLIDGKDSVQFLVSVKELLEDPSRLLLNV
CCCCCEEEEEHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
SIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKVVLEVPSPVDGVLSDIL
CEEEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCEEEECCCEEEEECCCCHHHHHHHHH
FNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPVKKEEIKISESESVSTKEDKSTSPVVR
HCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCHHHH
RMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQTAEVQKESSMKALSFGGREE
HHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHCHHHHHCCCCCC
KRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVMDMRSQYKDSFEKKHGVKLGFMS
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEHHHH
FFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIAVSTERGLVVPVIRDADQLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHEE
LAINDAASKARTGKLSMEEMQGGTFTITNGGVFGSLLATPIINPPQTGILGMHKIEERPI
EEEHHHHHHHHCCCCCHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCC
VEKGQIVIRPMMYVALSYDHRLIDGKDSVQFLVSVKELLEDPSRLLLNV
CCCCCEEEEEHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]