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The map label for this gene is trmB [H]

Identifier: 54293472

GI number: 54293472

Start: 567154

End: 567831

Strand: Direct

Name: trmB [H]

Synonym: lpl0521

Alternate gene names: 54293472

Gene position: 567154-567831 (Clockwise)

Preceding gene: 54293466

Following gene: 54293473

Centisome position: 16.95

GC content: 41.15

Gene sequence:

>678_bases
ATGCAACGTAAGATTAAAAGTTACGTGTTGCGCGCTGGGCGAATCAGTAACAGACAGCAACAAGGATTAGATCTCTGGCT
TGAAGACTATGAATTAAAATTTGATAGTCCCTCTCCCTGGAATTTTGCAAAAGAATTTGGCCGTCATGATGCGGATACTA
TTGTAGAGATTGGTTTTGGTATGGGTACCTCGTTGTTTGCCATGGCAATGAATAACCCTCAATGTAATTATCTGGGCATA
GAAGTACATAAAGCAGGCGTTGGTAGTCTGGTAGCGGACTTGCATGAGTACCAGATATCTAATGTACGGGTAGTAGCGCA
TGATGCAGTAGAAGTATTACAAACTAAAATACCAGAAAACTCGCTTGCCGGGGTTCAAATTTTCTTTCCCGATCCGTGGC
ATAAAAAGCGTCATCATAAGCGACGACTGATTCAATCCGAATTTATTCAAATGTTAGTAAAAAAAATCAGGCCATCTGGA
TTTATTCACTGTGCTACTGATTGGGAAGATTATGCAGAGCACATTTTAAATGTTTTATCTTCAGAATCCGCCTTGTTTAA
CCAACAAAAAGAGGGCGGATACTCACCACGCCCGGATTCAAGGCCGTTAACAAAATTTGAGCTGCGTGGTGAGCGTCTAG
GCCATGGGGTATGGGATCTGGTTTTTATAAAAAAATAA

Upstream 100 bases:

>100_bases
AACTGATTTTTCAGGGCTGTTGCCCAAACGGGTATCTGGTAAAAGTAGAATATTTAATCAAAATCAGTATAATCCTGAAA
TTTTATATTGAGTTATTGTG

Downstream 100 bases:

>100_bases
GGAAGTTATCTATGATAAAGCATGCGATTCTTGTTAGCGGATTGATTGTTTCCTTTTTAGCTTCAGCAGCTGAAATGTCA
TTTTTGGCCCAATTGACGGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAKEFGRHDADTIVEIGFGMGTSLFAMAMNNPQCNYLGI
EVHKAGVGSLVADLHEYQISNVRVVAHDAVEVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFELRGERLGHGVWDLVFIKK

Sequences:

>Translated_225_residues
MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAKEFGRHDADTIVEIGFGMGTSLFAMAMNNPQCNYLGI
EVHKAGVGSLVADLHEYQISNVRVVAHDAVEVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFELRGERLGHGVWDLVFIKK
>Mature_225_residues
MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAKEFGRHDADTIVEIGFGMGTSLFAMAMNNPQCNYLGI
EVHKAGVGSLVADLHEYQISNVRVVAHDAVEVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFELRGERLGHGVWDLVFIKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=220, Percent_Identity=51.8181818181818, Blast_Score=230, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 25874; Mature: 25874

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAKEFGRHDADTIVEIGFG
CCHHHHHHHHHHHCCCCHHHCCCCEEEECEEEEECCCCCCHHHHHHCCCCCHHHEEECCC
MGTSLFAMAMNNPQCNYLGIEVHKAGVGSLVADLHEYQISNVRVVAHDAVEVLQTKIPEN
CCHHHEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCC
SLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSGFIHCATDWEDYAEHILNVLS
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHC
SESALFNQQKEGGYSPRPDSRPLTKFELRGERLGHGVWDLVFIKK
CHHHHHHHHHCCCCCCCCCCCCCHHHECCHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAKEFGRHDADTIVEIGFG
CCHHHHHHHHHHHCCCCHHHCCCCEEEECEEEEECCCCCCHHHHHHCCCCCHHHEEECCC
MGTSLFAMAMNNPQCNYLGIEVHKAGVGSLVADLHEYQISNVRVVAHDAVEVLQTKIPEN
CCHHHEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCC
SLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSGFIHCATDWEDYAEHILNVLS
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHC
SESALFNQQKEGGYSPRPDSRPLTKFELRGERLGHGVWDLVFIKK
CHHHHHHHHHCCCCCCCCCCCCCHHHECCHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA