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The map label for this gene is dcoA [H]

Identifier: 54293458

GI number: 54293458

Start: 553683

End: 555473

Strand: Direct

Name: dcoA [H]

Synonym: lpl0507

Alternate gene names: 54293458

Gene position: 553683-555473 (Clockwise)

Preceding gene: 54293449

Following gene: 54293459

Centisome position: 16.55

GC content: 40.76

Gene sequence:

>1791_bases
ATGTCTAAAACGTTTATAACAGATGTCACCTTGCGTGATGCCCATCAATGCCTAATCGCTACAAGAATGCGTACTGAAGA
TATGTTGCCAATCTGTAATAAAATGGATGATGTAGGATTTTGGGCTATGGAAGTCTGGGGAGGCGCTACTTTTGATGCCT
GTTTGCGATTTTTAAAGGAAGATCCCTGGTCAAGATTGCGTCAACTTCGCCAAGCGTTACCGAATACTCAACTCTCTATG
CTATTAAGAGGTCAAAATTTGTTAGGCTATAGGAATTATGCAGATGATGTAGTCCGAGCTTTTGTTAAATTGGCCGTGAA
TAATGGAGTGGATGTATTTCGTGTGTTTGATGCTTTAAATGACGCGCGAAACCTTAAAGTAGCCATAGACGCGATTAAAT
CACATAAAAAACATGCCCAGGGAGCAATTTGTTACACAACCAGCCCGGTTCATACTTTGGACAATTTTTTGGAGCTAGGG
AAAAAATTGGCTGAAATGGGATGTGATAGTATCGCCATAAAAGATATGGCAGGTTTGCTAACCCCAGCGGTGACTGTCGA
ATTGTATGCCGGTTTAAAACAAGCGACTGGCTTACCTGTACATTTGCATAGTCATTCTACTTCCGGTTTGGCAAGTATAT
GCCATTATGAGGCCGTTTTATCGGGTTGTAATCACATTGATACAGCGATTTCATCTTTTTCTGGTGGTGCATCTCATCCA
CCTACGGAAGCATTGGTTGCCGCATTGACTGATACTCCGTATGACACGGAATTGGATTTAAATATTTTATTGGAAATAGA
CGATTATTTTAAAGCCGTCCGCAAGAAGTATTCTCAATTTGAAAGTGAGGCACAGAATATTGACCCTCGTGTTCAATTGT
ATCAAGTGCCAGGTGGCATGATCTCAAATCTGTATAATCAGTTAAGGGAGCAGAATGCATTAGACAAGATGGATGCTGTT
CACAAGGAAATCCCCCGCGTTCGTAAGGACTTGGGTTATCCGCCTTTGGTTACGCCAACATCTCAAATCGTTGGAACTCA
GGCAGTTATTAATGTTCTAACGGGAGAACGGTATAAAACAATAACCAATGAAGTGAAACTCTACTGTCAGGGAAAATATG
GCACTCCTCCTGGCAAAATTAGTTCTGCTTTACGGAAAAAAGCAATTGGACGAACAGAAGTGATTGAAGTCAGGCCTGGC
GATTTGTTACCTAATGAATTAGATCAATTGCAAAATGAAATAAGTGATTTGGCACTAAGTGATGAGGATGTTCTTCTTTA
TGCAATGTTTCCCGAAATTGGGCGCCAATTCTTAGAGCAGCGTAAAAATAATCAATTAATTCCTGAGCCATTATTGACTC
AATCAAGCGCACCAGATAATTCCGTCATGTCTGAGTTTGATATTATTCTTCATGGTGAGAGTTATCATGTAAAAGTAGCT
GGTTATGGAATGATTGAGCACGGCCAACAATCCTGTTTTTTGTGGGTTGATGGTGTTCCTGAAGAAGTTGTTGTGCAACA
TTCTGAACTTCATGACAAAATAGAGCGTTCATCTGTTAATAACAAGATAGGACCAGGAGATATTACTGTAGCTATCCCCG
GTTCTATTATTGCAATTCATGTTTCTTCTGGTGATGAAGTCAAGGCCGGGCAGGCTGTACTGGTTATTGAAGCAATGAAA
ATGGAGACAGAAATAAAAGCACCGGCAAATGGGGTGGTTGCAGAAATATTATGCCAAAAGGGAGACAAAGTGACTCCAGG
GCAAGTATTAATCAGAGTGGAGGTGAGTTAA

Upstream 100 bases:

>100_bases
GAAATTTTATATTTAGTAAAAAGCAATGGATTGTACTTAAAGAGTGTACCAATTTTAGGATTATGCTATTTTTATTTATT
TCTTGAGCCAGGAAGAGCTT

Downstream 100 bases:

>100_bases
CGGGTAGTTAAGTGTATTGTTTTCACTACTTGTTTTTGCCTATTCCAAAATTATGTAAAAATTTTTATGTTATTAAAAAT
AACAACAAGGTTAATTAGCA

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAMEVWGGATFDACLRFLKEDPWSRLRQLRQALPNTQLSM
LLRGQNLLGYRNYADDVVRAFVKLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELG
KKLAEMGCDSIAIKDMAGLLTPAVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEAVLSGCNHIDTAISSFSGGASHP
PTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKKYSQFESEAQNIDPRVQLYQVPGGMISNLYNQLREQNALDKMDAV
HKEIPRVRKDLGYPPLVTPTSQIVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRTEVIEVRPG
DLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEPLLTQSSAPDNSVMSEFDIILHGESYHVKVA
GYGMIEHGQQSCFLWVDGVPEEVVVQHSELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSSGDEVKAGQAVLVIEAMK
METEIKAPANGVVAEILCQKGDKVTPGQVLIRVEVS

Sequences:

>Translated_596_residues
MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAMEVWGGATFDACLRFLKEDPWSRLRQLRQALPNTQLSM
LLRGQNLLGYRNYADDVVRAFVKLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELG
KKLAEMGCDSIAIKDMAGLLTPAVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEAVLSGCNHIDTAISSFSGGASHP
PTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKKYSQFESEAQNIDPRVQLYQVPGGMISNLYNQLREQNALDKMDAV
HKEIPRVRKDLGYPPLVTPTSQIVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRTEVIEVRPG
DLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEPLLTQSSAPDNSVMSEFDIILHGESYHVKVA
GYGMIEHGQQSCFLWVDGVPEEVVVQHSELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSSGDEVKAGQAVLVIEAMK
METEIKAPANGVVAEILCQKGDKVTPGQVLIRVEVS
>Mature_595_residues
SKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAMEVWGGATFDACLRFLKEDPWSRLRQLRQALPNTQLSML
LRGQNLLGYRNYADDVVRAFVKLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELGK
KLAEMGCDSIAIKDMAGLLTPAVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEAVLSGCNHIDTAISSFSGGASHPP
TEALVAALTDTPYDTELDLNILLEIDDYFKAVRKKYSQFESEAQNIDPRVQLYQVPGGMISNLYNQLREQNALDKMDAVH
KEIPRVRKDLGYPPLVTPTSQIVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRTEVIEVRPGD
LLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEPLLTQSSAPDNSVMSEFDIILHGESYHVKVAG
YGMIEHGQQSCFLWVDGVPEEVVVQHSELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSSGDEVKAGQAVLVIEAMKM
ETEIKAPANGVVAEILCQKGDKVTPGQVLIRVEVS

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=616, Percent_Identity=32.4675324675325, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI106049295, Length=616, Percent_Identity=32.4675324675325, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI106049292, Length=616, Percent_Identity=32.4675324675325, Blast_Score=300, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17562816, Length=615, Percent_Identity=33.1707317073171, Blast_Score=307, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6321376, Length=625, Percent_Identity=33.12, Blast_Score=317, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6319695, Length=620, Percent_Identity=32.9032258064516, Blast_Score=314, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24652212, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24652210, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24652214, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI19921944, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24652216, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI281363050, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24652224, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24652222, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24652220, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24652218, Length=618, Percent_Identity=33.495145631068, Blast_Score=308, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 65685; Mature: 65554

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAMEVWGGATFDACLRFLKE
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEHHHCCCCCHHHHHHHHHC
DPWSRLRQLRQALPNTQLSMLLRGQNLLGYRNYADDVVRAFVKLAVNNGVDVFRVFDALN
CHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
DARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELGKKLAEMGCDSIAIKDMAGLL
CCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TPAVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEAVLSGCNHIDTAISSFSGGASHP
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
PTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKKYSQFESEAQNIDPRVQLYQVPGGM
CHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHH
ISNLYNQLREQNALDKMDAVHKEIPRVRKDLGYPPLVTPTSQIVGTQAVINVLTGERYKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
ITNEVKLYCQGKYGTPPGKISSALRKKAIGRTEVIEVRPGDLLPNELDQLQNEISDLALS
HHHCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
DEDVLLYAMFPEIGRQFLEQRKNNQLIPEPLLTQSSAPDNSVMSEFDIILHGESYHVKVA
CCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHEEEEEECCCEEEEEE
GYGMIEHGQQSCFLWVDGVPEEVVVQHSELHDKIERSSVNNKIGPGDITVAIPGSIIAIH
ECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEE
VSSGDEVKAGQAVLVIEAMKMETEIKAPANGVVAEILCQKGDKVTPGQVLIRVEVS
ECCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
SKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAMEVWGGATFDACLRFLKE
CCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEHHHCCCCCHHHHHHHHHC
DPWSRLRQLRQALPNTQLSMLLRGQNLLGYRNYADDVVRAFVKLAVNNGVDVFRVFDALN
CHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
DARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELGKKLAEMGCDSIAIKDMAGLL
CCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TPAVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEAVLSGCNHIDTAISSFSGGASHP
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
PTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKKYSQFESEAQNIDPRVQLYQVPGGM
CHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHH
ISNLYNQLREQNALDKMDAVHKEIPRVRKDLGYPPLVTPTSQIVGTQAVINVLTGERYKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
ITNEVKLYCQGKYGTPPGKISSALRKKAIGRTEVIEVRPGDLLPNELDQLQNEISDLALS
HHHCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
DEDVLLYAMFPEIGRQFLEQRKNNQLIPEPLLTQSSAPDNSVMSEFDIILHGESYHVKVA
CCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHEEEEEECCCEEEEEE
GYGMIEHGQQSCFLWVDGVPEEVVVQHSELHDKIERSSVNNKIGPGDITVAIPGSIIAIH
ECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEE
VSSGDEVKAGQAVLVIEAMKMETEIKAPANGVVAEILCQKGDKVTPGQVLIRVEVS
ECCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]