Click here to switch to the map view.

The map label for this gene is pncA [H]

Identifier: 54293275

GI number: 54293275

Start: 368964

End: 369584

Strand: Direct

Name: pncA [H]

Synonym: lpl0323

Alternate gene names: 54293275

Gene position: 368964-369584 (Clockwise)

Preceding gene: 54293274

Following gene: 54293278

Centisome position: 11.03

GC content: 36.55

Gene sequence:

>621_bases
ATGAAGACTTTAATCATCCTCGATGTTCAAAATGATTTCATGCGAGGCGGCTCACTGGGAGTGCCGAATGGTGATGCGAT
TGTTCCTGTTATTAACAGCATTTTGCATTGTTTTGATCTGATTGTTGCTTCACAGGATTGGCATCCCTCTAATCATATCA
GTTTCGCCTCCAATCATACTGGCAGGAAACCTTTTGAAAAAATCAAGCTGGGGAATTTAGAACAAATTTTATGGCCAGAT
CATTGTGTTCAGGGTAGTATTGGAGCGCAATTTCATGCGCAATTAAATACTAATCCTATTGAAGCTATTTTTCGAAAAGG
AACTGACCCCAATATAGATAGCTACAGTGGTTTTTATGATAATTTGCATAAAAAAACCACAGGGCTTGCCGGTTATCTGC
GCGAAAAAGGCGCTAGAGAACTTTATTTTTGTGGTTTGTGCGCTGATATTTGTGTTTATTTTACAATTAAGGATGCTCTC
AGTCAGGGATTTGATTGCTATTTGATTGAGGATGCAACTCAAGCGCTTGTGAAAGATGATTTCATAAAAATAAAAAAAGA
ATTGATAAAACAGGGCTCTCACATAATTAACAGTTCAAATTTGCTTAATAATTTACAATAA

Upstream 100 bases:

>100_bases
TACAAGCGTTTTATTAATCCTCATATCTATAAAGTGGGTTTAAGTACCGAGCTTAAAATGAAGAGAGATCAACTGATAAC
TGAACACTCAAGATAATTTT

Downstream 100 bases:

>100_bases
GAAGGTCTGATTTTTTAAATTTGCAGCTTTGAGCTCACCAATTAATGATGGAACTCGCTGTCGAAGGAACCCCGTCGTAT
TTTTAAGACCCGCTACCATA

Product: nicotinamidase/ pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKTLIILDVQNDFMRGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHTGRKPFEKIKLGNLEQILWPD
HCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
SQGFDCYLIEDATQALVKDDFIKIKKELIKQGSHIINSSNLLNNLQ

Sequences:

>Translated_206_residues
MKTLIILDVQNDFMRGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHTGRKPFEKIKLGNLEQILWPD
HCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
SQGFDCYLIEDATQALVKDDFIKIKKELIKQGSHIINSSNLLNNLQ
>Mature_206_residues
MKTLIILDVQNDFMRGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHTGRKPFEKIKLGNLEQILWPD
HCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
SQGFDCYLIEDATQALVKDDFIKIKKELIKQGSHIINSSNLLNNLQ

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=179, Percent_Identity=44.6927374301676, Blast_Score=161, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI193206735, Length=220, Percent_Identity=35.4545454545455, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=220, Percent_Identity=35.4545454545455, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=219, Percent_Identity=33.7899543378995, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321401, Length=217, Percent_Identity=33.1797235023041, Blast_Score=113, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648247, Length=229, Percent_Identity=36.2445414847162, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22984; Mature: 22984

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLIILDVQNDFMRGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHT
CCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
GRKPFEKIKLGNLEQILWPDHCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYD
CCCCHHHHCCCCCHHHCCCCHHCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHH
NLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDALSQGFDCYLIEDATQALVKDD
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
FIKIKKELIKQGSHIINSSNLLNNLQ
HHHHHHHHHHCCCCEECCHHHHHCCC
>Mature Secondary Structure
MKTLIILDVQNDFMRGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHT
CCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
GRKPFEKIKLGNLEQILWPDHCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYD
CCCCHHHHCCCCCHHHCCCCHHCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHH
NLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDALSQGFDCYLIEDATQALVKDD
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
FIKIKKELIKQGSHIINSSNLLNNLQ
HHHHHHHHHHCCCCEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]