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The map label for this gene is lplA

Identifier: 54020454

GI number: 54020454

Start: 377135

End: 378166

Strand: Reverse

Name: lplA

Synonym: mhp323

Alternate gene names: 54020454

Gene position: 378166-377135 (Counterclockwise)

Preceding gene: 54020455

Following gene: 54020453

Centisome position: 42.36

GC content: 27.81

Gene sequence:

>1032_bases
ATGTACTTAATCGAACCAAAACGCAATGGAAAATGAGTTTTTGACGGAGCAATTTTGCTTGCAATTCAATATTGAGCAAT
CAAAAACTTAAAACTAGATGAAACAATTGTTTTTCCTTATATTTGTGATCCACATGTACAAATTGGTTATTTCCAAAATC
CAAGTGTTGAAGTTAACCTTGAACTTTTAAAGCAAAAAAATATTGAAGTTGTTAGACGCGACACTGGTGGTGGTGCAATT
TATTTAGATAGAAATGGAGTAAATTTTTGTTTTTCATTCCCATATGAAAAAAATAAAAATTTACTCGGAAATTATGCACA
ATTTTACGATCCTGTTATTAAAGTTTTGCAAAACATAGGGATAAAAAATGTGCAATTTAGTGGAAAAAATGACCTTCAAA
TTGAAGGAAAAAAAGTTTCTGGTGCAGCAATGTCGCTTGTAAATGATAGAATTTATGCTGGTTTTTCACTTTTATATGAT
GTAGATTTTGATTTTATTGGCAAAATTCTAACTCCAAACCAGAAAAAAATTGAAGCAAAAGGGATAAAATCTGTTTCTCA
ACGGGTAACTAATTTAAAAAATAAGCTTTCAAAAGAATATCAAAATTTTTCAATTTTTGAAATAAAAGACCTATTTTTGA
CCGAATTTTTGAAAGTAAATTCAGTAGAAAAGTTTAAAAAATACGAGCTTACTGATAGCGATTGAGTCCAAATTGACAAA
ATGGTCGCTGAAAAATACAAAAATTGGGATTTTGTTTGAGGACTTAGCCCAAATTATTCCTTCAATAGATCAATTAGAAC
AAAAGTTGGAACAATAACATTTTCTTTAGAAATTAATGAAGGAAAAATTAGTAAAATTAAAATTTCAGGTGATTTTTTCC
CGAAAAAGTCGCTTTTAGAGTTAGAAAATTTCTTAATTGGGACAAAATTAACTCAAGATCAACTATTAAATCGGCTAAAA
GATGCAAAATTAGATGATTATTTTAGCCAAAAAATTGATGAAGAAGAAATTTGTAACTTACTTTTAAACTAA

Upstream 100 bases:

>100_bases
AGAAAAAATACCGTACTAACAAGGATATTCTTGAAAAATCCTATTTTTTTCATGAGGATATTAAATTACTTCTACAAAAA
ATATCAGAACAGGAATAAAA

Downstream 100 bases:

>100_bases
ATATGAAAAACAAACTTTTTAAACCGTATCAAATTGGTAATTTCACATTAAAAAACCGATTTGTTTTTAGCCCAATTAAT
CTTAATCTTTCTGAAATGGG

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MYLIEPKRNGKWVFDGAILLAIQYWAIKNLKLDETIVFPYICDPHVQIGYFQNPSVEVNLELLKQKNIEVVRRDTGGGAI
YLDRNGVNFCFSFPYEKNKNLLGNYAQFYDPVIKVLQNIGIKNVQFSGKNDLQIEGKKVSGAAMSLVNDRIYAGFSLLYD
VDFDFIGKILTPNQKKIEAKGIKSVSQRVTNLKNKLSKEYQNFSIFEIKDLFLTEFLKVNSVEKFKKYELTDSDWVQIDK
MVAEKYKNWDFVWGLSPNYSFNRSIRTKVGTITFSLEINEGKISKIKISGDFFPKKSLLELENFLIGTKLTQDQLLNRLK
DAKLDDYFSQKIDEEEICNLLLN

Sequences:

>Translated_343_residues
MYLIEPKRNGK*VFDGAILLAIQY*AIKNLKLDETIVFPYICDPHVQIGYFQNPSVEVNLELLKQKNIEVVRRDTGGGAI
YLDRNGVNFCFSFPYEKNKNLLGNYAQFYDPVIKVLQNIGIKNVQFSGKNDLQIEGKKVSGAAMSLVNDRIYAGFSLLYD
VDFDFIGKILTPNQKKIEAKGIKSVSQRVTNLKNKLSKEYQNFSIFEIKDLFLTEFLKVNSVEKFKKYELTDSD*VQIDK
MVAEKYKNWDFV*GLSPNYSFNRSIRTKVGTITFSLEINEGKISKIKISGDFFPKKSLLELENFLIGTKLTQDQLLNRLK
DAKLDDYFSQKIDEEEICNLLLN
>Mature_343_residues
MYLIEPKRNGK*VFDGAILLAIQY*AIKNLKLDETIVFPYICDPHVQIGYFQNPSVEVNLELLKQKNIEVVRRDTGGGAI
YLDRNGVNFCFSFPYEKNKNLLGNYAQFYDPVIKVLQNIGIKNVQFSGKNDLQIEGKKVSGAAMSLVNDRIYAGFSLLYD
VDFDFIGKILTPNQKKIEAKGIKSVSQRVTNLKNKLSKEYQNFSIFEIKDLFLTEFLKVNSVEKFKKYELTDSD*VQIDK
MVAEKYKNWDFV*GLSPNYSFNRSIRTKVGTITFSLEINEGKISKIKISGDFFPKKSLLELENFLIGTKLTQDQLLNRLK
DAKLDDYFSQKIDEEEICNLLLN

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21729884, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21729882, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21729880, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21729878, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790846, Length=336, Percent_Identity=26.4880952380952, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=29.9212598425197, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322415, Length=157, Percent_Identity=29.2993630573248, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28573464, Length=280, Percent_Identity=24.6428571428571, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38869; Mature: 38869

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLIEPKRNGKVFDGAILLAIQYAIKNLKLDETIVFPYICDPHVQIGYFQNPSVEVNLEL
CEEECCCCCCCEECHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCEEEEEEE
LKQKNIEVVRRDTGGGAIYLDRNGVNFCFSFPYEKNKNLLGNYAQFYDPVIKVLQNIGIK
ECCCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
NVQFSGKNDLQIEGKKVSGAAMSLVNDRIYAGFSLLYDVDFDFIGKILTPNQKKIEAKGI
EEEECCCCCEEEECEEECHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCHHHHHHHH
KSVSQRVTNLKNKLSKEYQNFSIFEIKDLFLTEFLKVNSVEKFKKYELTDSDVQIDKMVA
HHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHH
EKYKNWDFVGLSPNYSFNRSIRTKVGTITFSLEINEGKISKIKISGDFFPKKSLLELENF
HHHCCCEEEECCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
LIGTKLTQDQLLNRLKDAKLDDYFSQKIDEEEICNLLLN
HHCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MYLIEPKRNGKVFDGAILLAIQYAIKNLKLDETIVFPYICDPHVQIGYFQNPSVEVNLEL
CEEECCCCCCCEECHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCEEEEEEE
LKQKNIEVVRRDTGGGAIYLDRNGVNFCFSFPYEKNKNLLGNYAQFYDPVIKVLQNIGIK
ECCCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
NVQFSGKNDLQIEGKKVSGAAMSLVNDRIYAGFSLLYDVDFDFIGKILTPNQKKIEAKGI
EEEECCCCCEEEECEEECHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCHHHHHHHH
KSVSQRVTNLKNKLSKEYQNFSIFEIKDLFLTEFLKVNSVEKFKKYELTDSDVQIDKMVA
HHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHH
EKYKNWDFVGLSPNYSFNRSIRTKVGTITFSLEINEGKISKIKISGDFFPKKSLLELENF
HHHCCCEEEECCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
LIGTKLTQDQLLNRLKDAKLDDYFSQKIDEEEICNLLLN
HHCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]