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The map label for this gene is baiH

Identifier: 54020453

GI number: 54020453

Start: 375960

End: 377132

Strand: Reverse

Name: baiH

Synonym: mhp322

Alternate gene names: 54020453

Gene position: 377132-375960 (Counterclockwise)

Preceding gene: 54020454

Following gene: 54020452

Centisome position: 42.24

GC content: 30.95

Gene sequence:

>1173_bases
ATGAAAAACAAACTTTTTAAACCGTATCAAATTGGTAATTTCACATTAAAAAACCGATTTGTTTTTAGCCCAATTAATCT
TAATCTTTCTGAAATGGGCCAAGTTTGCCAAGCGGATTTAGATTTTTTTAGCGCTAGAGCCCATAGTGCACCAATGACAA
TTACAGGGGGAGTATATATTTGTAAATCAGGTAGTTTGTTCAAATATGGTCATAGTATCGAAAATGATAGCGATATTGAA
GGTCTAGCAAAACTTGCAAGGGCAATAAAATCAAAGGGAAGTATCGCAATTTTGCAATTAATTCATGCTGGTTCTCACTC
TGTTTCAAAAATTGAAAAAGATGGTTTTTGCTATGGACCTAGTGCTGAAAAGCGAAATTATCCTGTAAAATACAAGGTTT
ATCGTCTTAGACAAAAGCAAATTAAAACCATTGTTAGACAATATGCAGATGCTTGTCTAAGGGCAATTAAGGCAGGTTTT
GATGGAGTTGAAATTTCTTCGGCCCAAAAATTATTACCTCAAGAATTTTTCTCAAATTATACTAACAAGAGAAATGATAA
ATATGGCTGTCAAAATATTGAAAATAGATCTCGGTTTTTGCTAGAAATATTAGAAAAAGTACAAAAAACTATTTTAAAAT
CAAAAAATCCTAATTTTTTGCTAGGTTACCGGGCAACACCTGAGGAACGAAGAGGCGATAATTTAGGTTATACAATCGAA
AATTTCCTAGATTTCTTAGATTTAATTGAAAAAAGGGGGATAAAAATTTCTTACCTTGCAATTGCCGGTTGAGGTTTTGA
CGTTTTTCGTGACAAAGTTCGGTCAAAATCAAATAATTTTAACCGTTTAATTTCTGATGTAGTTTATGAAAAATTGCAAG
GAAAAATCCCTGTTATTGCTAGTGGTTCAATAAATTCCTTAGAAAAATCAGTTAAAGCTATTCAATTTGCTGATTTTATC
GGAATTTCTTCAGCCTTTGTGCCTGATCCTGATTTTGTTGAAAAAATTGAGAAAAATCAGCCAATAAAATTAAGTCTTGA
GCCGAAAGATTTTGCAAAATTAAAAATAAGTAAAAACGCATTTAAAGAAATTGGTCATTATTTTGAATATGGCAAATCTT
TACCTGAAAATGCTCGAAAAACTATTTTAAATAATTCAAAAAAAGCAGAATAA

Upstream 100 bases:

>100_bases
AAGATCAACTATTAAATCGGCTAAAAGATGCAAAATTAGATGATTATTTTAGCCAAAAAATTGATGAAGAAGAAATTTGT
AACTTACTTTTAAACTAAAT

Downstream 100 bases:

>100_bases
TTTTTTTCTTTGAAAAATCAATAAAAAAATATATAATTATACATTTATTAATTATATATTCCCAAATTCAAAATATAAAA
GCAACACACAGATATTTTTA

Product: NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYICKSGSLFKYGHSIENDSDIE
GLAKLARAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGPSAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGF
DGVEISSAQKLLPQEFFSNYTNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTIE
NFLDFLDLIEKRGIKISYLAIAGWGFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIASGSINSLEKSVKAIQFADFI
GISSAFVPDPDFVEKIEKNQPIKLSLEPKDFAKLKISKNAFKEIGHYFEYGKSLPENARKTILNNSKKAE

Sequences:

>Translated_390_residues
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYICKSGSLFKYGHSIENDSDIE
GLAKLARAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGPSAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGF
DGVEISSAQKLLPQEFFSNYTNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTIE
NFLDFLDLIEKRGIKISYLAIAG*GFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIASGSINSLEKSVKAIQFADFI
GISSAFVPDPDFVEKIEKNQPIKLSLEPKDFAKLKISKNAFKEIGHYFEYGKSLPENARKTILNNSKKAE
>Mature_390_residues
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYICKSGSLFKYGHSIENDSDIE
GLAKLARAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGPSAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGF
DGVEISSAQKLLPQEFFSNYTNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTIE
NFLDFLDLIEKRGIKISYLAIAG*GFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIASGSINSLEKSVKAIQFADFI
GISSAFVPDPDFVEKIEKNQPIKLSLEPKDFAKLKISKNAFKEIGHYFEYGKSLPENARKTILNNSKKAE

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=341, Percent_Identity=23.7536656891496, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787939, Length=371, Percent_Identity=23.989218328841, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=340, Percent_Identity=26.1764705882353, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=269, Percent_Identity=29.368029739777, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=332, Percent_Identity=25, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=302, Percent_Identity=26.8211920529801, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=307, Percent_Identity=24.7557003257329, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=283, Percent_Identity=26.8551236749117, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17566914, Length=196, Percent_Identity=29.5918367346939, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=361, Percent_Identity=26.3157894736842, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321973, Length=371, Percent_Identity=26.6846361185984, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 43978; Mature: 43978

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYI
CCCCCCCCEECCCEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
CKSGSLFKYGHSIENDSDIEGLAKLARAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGP
ECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCEECC
SAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGFDGVEISSAQKLLPQEFFSNY
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCEEHH
NFLDFLDLIEKRGIKISYLAIAGGFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIAS
HHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEC
GSINSLEKSVKAIQFADFIGISSAFVPDPDFVEKIEKNQPIKLSLEPKDFAKLKISKNAF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHEEECHHHH
KEIGHYFEYGKSLPENARKTILNNSKKAE
HHHHHHHHHCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNKLFKPYQIGNFTLKNRFVFSPINLNLSEMGQVCQADLDFFSARAHSAPMTITGGVYI
CCCCCCCCEECCCEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
CKSGSLFKYGHSIENDSDIEGLAKLARAIKSKGSIAILQLIHAGSHSVSKIEKDGFCYGP
ECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCEECC
SAEKRNYPVKYKVYRLRQKQIKTIVRQYADACLRAIKAGFDGVEISSAQKLLPQEFFSNY
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TNKRNDKYGCQNIENRSRFLLEILEKVQKTILKSKNPNFLLGYRATPEERRGDNLGYTIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCEEHH
NFLDFLDLIEKRGIKISYLAIAGGFDVFRDKVRSKSNNFNRLISDVVYEKLQGKIPVIAS
HHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEC
GSINSLEKSVKAIQFADFIGISSAFVPDPDFVEKIEKNQPIKLSLEPKDFAKLKISKNAF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHEEECHHHH
KEIGHYFEYGKSLPENARKTILNNSKKAE
HHHHHHHHHCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]